Results 31 to 40 of about 1,105,287 (296)
Sensors, 2013 Förster resonance energy transfer (FRET) technology has been widely used in biological and biomedical research. This powerful tool can elucidate protein interactions in either a dynamic or steady state.
Wensheng Xiang +6 more
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Spectral unmixing: analysis of performance in the olfactory bulb in vivo. [PDF]
PLoS ONE, 2009 BACKGROUND: The generation of transgenic mice expressing combinations of fluorescent proteins has greatly aided the reporting of activity and identification of specific neuronal populations. Methods capable of separating multiple overlapping fluorescence
Mathieu Ducros +5 more
doaj +1 more source
eLife, 2021 The force-induced unfolding and refolding of proteins is speculated to be a key mechanism in the sensing and transduction of mechanical signals in the living cell. Yet, little evidence has been gathered for its existence in vivo.
Daniel Porto +7 more
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FRET imaging of calcium signaling in live cells in the microenvironment [PDF]
Integr. Biol., 2013 The microenvironment has been shown to regulate cellular functions including cell growth, differentiation, proliferation, migration, cancer development and metastasis. However, the underlying molecular mechanism remains largely unclear. We have integrated micro-pattern technology and molecular biosensors based on fluorescence resonance energy transfer (
Tongcheng, Qian +5 more
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No better time to FRET: shedding light on host pathogen interactions [PDF]
, 2010 Understanding the spatio-temporal subversion of host cell signaling by bacterial virulence factors is key to combating infectious diseases. Following a recent study by Buntru and co-workers published in BMC Biology, we review how fluorescence (Forster ...
Leong, J.M. +5 more
core +1 more source
Förster Resonance Energy Transfer between Quantum Dot Donors and Quantum Dot Acceptors
Sensors, 2015 Förster (or fluorescence) resonance energy transfer amongst semiconductor quantum dots (QDs) is reviewed, with particular interest in biosensing applications.
Kenny F. Chou, Allison M. Dennis
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Scientific Reports, 2023 FRET-based sensors are utilized for real-time measurements of cellular tension. However, transfection of the sensor gene shows low efficacy and is only effective for a short period.
Junfeng Wang +7 more
doaj +1 more source
Versatile Cell and Animal Models for Advanced Investigation of Lead Poisoning
Biosensors, 2021 The heavy metal, lead (Pb) can irreversibly damage the human nervous system. To help understand Pb-induced damage, we applied a genetically encoded Förster resonance energy transfer (FRET)-based Pb biosensor Met-lead 1.44 M1 to two living systems to ...
De-Ming Yang, Yu-Fen Chang
doaj +1 more source
Quantitative analysis of chromatin compaction in living cells using FLIM-FRET [PDF]
, 2009 We present a quantitative Forster resonance energy transfer (FRET)-based assay using multiphoton fluorescence lifetime imaging microscopy (FLIM) to measure chromatin compaction at the scale of nucleosomal arrays in live cells. The assay uses a human cell
Angus I. Lamond +11 more
core +1 more source
Encoding multiple virtual signals in DNA barcodes with single-molecule FRET [PDF]
Nano Letters, 2020 ABSTRACT DNA barcoding provides a way to label a huge number of different biological molecules using the extreme programmability in DNA sequence synthesis. Fluorescence imaging is an easy-to-access method to detect individual DNA barcodes, which can be scaled up to a massively high-throughput format.
Sung Hyun Kim +3 more
openaire +3 more sources