Results 11 to 20 of about 1,105,287 (296)

Analysis of FRET Signals in the Presence of Free Donors and Acceptors [PDF]

Biophysical Journal, 2007
A method for spectral analysis of Förster resonance energy transfer (FRET) signals is presented, taking into consideration both the contributions of unpaired donor and acceptor fluorophores and the influence of incomplete labeling of the interacting ...
Andre Zeug, Evgeni Ponimaskin, Kobe, F., Wlodarczyk, Jakub, Erwin Neher, Zeug, Andre, Ponimaskin, E., Wlodarczyk, J., Woehler, Andrew, Neher, E., Kobe, Fritz, Fritz Kobe, Zeug, A., Neher, Erwin, Jakub Wlodarczyk, Ponimaskin, Evgeni G., Andrew Woehler, Woehler, A.   +17 more
core   +6 more sources

FRET: signals hidden within the noise. [PDF]

Cytometry A, 2014
F€ orster resonance energy transfer (FRET) is a physical phenomenon that has been of incredible utility to the scientific community, enabling the study of a wide range of nanoscale, distance-dependent measurements. FRET occurs when energy is nonradiatively transferred from an excited fluorophore (the donor fluorophore) to a second fluorophore in near ...
Leavesley SJ, Rich TC.
europepmc   +4 more sources

Head‐to‐head comparison between ACQ and FRET in probing polymeric micelles

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Environmentally responsive fluorophores, such as those with aggregation‐caused quenching (ACQ) and Förster resonance energy transfer (FRET) properties, are widely used to track nanocarriers while minimizing artifacts.
Runtong Zhang, Chang Liu, Ruonan Lu, Yuanmei Yang, Zichen Zhang, Yifan Cai, Haisheng He, Yi Lu, Aun Raza, Wei Wu   +9 more
doaj   +2 more sources

Mutant p53^R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation. [PDF]

Mol Oncol
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Lim MC, Guo AK, Itahana Y, Kannan S, Goh V, Ong M, Tay TYW, Ye W, Krishnan SR, Nishiyama J, Verma CS, Itahana K.   +11 more
europepmc   +2 more sources

Transmitting FRET signals to nerve cells [PDF]

Proceedings of the 5th ACM International Conference on Nanoscale Computing and Communication, 2018
This paper is concerned with a novel method allowing communication between FRET nanonetworks and nerve cells. It is focused on two system components: fluorophores and channelrhodopsins which serve as transmitters and receivers, respectively. Channelrhodopsins are used here also as a FRET signal-to-voltage converter. The trade-off between throughput and
Jakub Kmiecik, Pawel Kulakowski, Krzysztof Wójcik, Andrzej Jajszczyk   +3 more
openaire   +4 more sources

A yeast FRET biosensor enlightens cAMP signalling [PDF]

Molecular Biology of the Cell, 2019
Abstract The cAMP-PKA signalling cascade in budding yeast regulates adaptation to changing environments. We developed yEPAC, a FRET-based biosensor for cAMP measurements in yeast. We used this sensor with flow cytometry for high-throughput single cell-level quantification during dynamic changes in response to sudden nutrient transitions.
Dennis Botman, Tom G. O’Toole, Joachim Goedhart, Frank J. Bruggeman, Johan H. van Heerden, Bas Teusink   +5 more
openaire   +6 more sources

Dynamic FRET-FLIM based screening of signal transduction pathways [PDF]

Scientific Reports, 2021
Abstract Fluorescence Lifetime Imaging (FLIM) is an intrinsically quantitative method to screen for protein–protein interactions and is frequently used to record the outcome of signal transduction events. With new highly sensitive and photon efficient FLIM instrumentation, the technique also becomes attractive to ...
Harkes, Rolf, Kukk, Olga, Mukherjee, Sravasti, Klarenbeek, Jeffrey, van den Broek, Bram, Jalink, Kees   +5 more
openaire   +6 more sources

Application of FRET Biosensors in Mechanobiology and Mechanopharmacological Screening

Frontiers in Bioengineering and Biotechnology, 2020
Extensive studies have shown that cells can sense and modulate the biomechanical properties of the ECM within their resident microenvironment. Thus, targeting the mechanotransduction signaling pathways provides a promising way for disease intervention ...
Longwei Liu, Fangchao He, Yiyan Yu, Yingxiao Wang   +3 more
doaj   +1 more source

FRET from single to multiplexed signaling events [PDF]

Biophysical Reviews, 2017
Förster resonance energy transfer (FRET) is a powerful tool for the visualization of molecular signaling events such as protein activities and interactions in cells. In its different implementations, FRET microscopy has been mainly used for monitoring single events.
Gertrude Bunt, Fred S. Wouters
openaire   +2 more sources

Some secrets of fluorescent proteins: distinct bleaching in various mounting fluids and photoactivation of cyan fluorescent proteins at YFP-excitation. [PDF]

PLoS ONE, 2011
BACKGROUND: The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g.
Naila Malkani, Johannes A Schmid
doaj   +1 more source