Results 21 to 30 of about 1,105,287 (296)

Robust calibration and quantification of FRET signals using multiplexed biosensor barcoding. [PDF]

iScience
Förster resonance energy transfer (FRET) between fluorescent proteins (FPs) underpins many genetically encoded fluorescent biosensors for monitoring biochemical activities in live cells. However, the FRET ratio (acceptor-to-donor signal ratio), commonly used as a proxy for FRET efficiency, is highly sensitive to imaging parameters, complicating data ...
Wu JW, Yang JM, Chen CC, Au G, Wang S, Xu Y, Chern GW, Huang CH.   +7 more
europepmc   +3 more sources

smXplorer/IVIS-FRET-Analysis: First release of IVIS FRET Analysis

, 2023
First release associated with the article "in vivo quantitative FRET small animal imaging: intensity versus lifetime-based FRET" by Smith et al.
smXplorer
core   +1 more source

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs [PDF]

, 2010
Förster resonance energy transfer (FRET) is a powerful method for obtaining information about small-scale lengths between biomacromolecules. Visible fluorescent proteins (VFPs) are widely used as spectrally different FRET pairs, where one VFP acts as a ...
Birch, D.J.S., Laptenok, S., Visser, N.V., Hoek, A., van, Laptenok, S.P., Borst, J.W., Visser, A.J.W.G., van Hoek, A., Brochon, J.C.   +8 more
core   +1 more source

Differential RhoA dynamics in migratory and stationary cells measured by FRET and automated image analysis. [PDF]

PLoS ONE, 2008
Genetically-encoded biosensors based on fluorescence resonance energy transfer (FRET) have been widely applied to study the spatiotemporal regulation of molecular activity in live cells with high resolution.
John Paul Eichorst, Shaoying Lu, Jing Xu, Yingxiao Wang   +3 more
doaj   +1 more source

Structural Changes of Yellow Cameleon Domains Observed by Quantitative FRET Analysis and Polarized Fluorescence Correlation Spectroscopy [PDF]

, 2008
Förster resonance energy transfer (FRET) is a widely used method for monitoring interactions between or within biological macromolecules conjugated with suitable donor-acceptor pairs.
Laptenok, S., Visser, N.V., Kalinin, S., Hornen, H., Hoek, A., van, Kühnemuth, R., Borst, J.W., van Hoek, A., Westphal, A.H., Aker, J., Aker, J.C.M., Kyhnemuth, R., Laptenok, S.P., Seidel, C.A.M., Visser, A.J.W.G.   +14 more
core   +1 more source

FRET-FISH datasets

, 2021
We designed FRET-FISH probes for optimization of the design with the first being on MYC gene on human HAP1 cell lines and Ogt on mouse NIH3T3 and MEF. Thereafter, the optimized FRET-FISH design targeted Magix, Kdm5c, Atp2b3, Tent5d, Ddx3x and Pbd1c on ...
Szymon Berezicki (13032543), Katarina Gradin (13897536), Magda Bienko (11771891), Nicola Crosetto (10922484), Erik Wernersson (11771885), Ana Faustino Mota (10350002), Luuk Harbers (11956331), Xiaoze Li (11771881), Christiane Peuckert (842143)   +8 more
core   +1 more source

Developing Fast Fluorescent Protein Voltage Sensors by Optimizing FRET Interactions. [PDF]

PLoS ONE, 2015
FRET (Förster Resonance Energy Transfer)-based protein voltage sensors can be useful for monitoring neuronal activity in vivo because the ratio of signals between the donor and acceptor pair reduces common sources of noise such as heart beat artifacts ...
Uhna Sung, Masoud Sepehri-Rad, Hong Hua Piao, Lei Jin, Thomas Hughes, Lawrence B Cohen, Bradley J Baker   +6 more
doaj   +1 more source

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]

, 2012
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Ankerhold, Richard, Richard Ankerhold, Drummen, Gregor P. C., Gregor P. C. Drummen, Hellen C. Ishikawa-Ankerhold, Ishikawa-Ankerhold, Hellen C.   +5 more
core   +1 more source

Signals of Popularity

, 2023
In this project we focus on the interplay between volume of sales and number of reviews and explore what happens when these signals are perceived as congruent versus ...
Signals of Popularity
core   +1 more source

Live Cell Imaging of Src/FAK Signaling by FRET [PDF]

Cellular and Molecular Bioengineering, 2011
The Src/FAK complex is involved in many signaling pathways and plays crucial roles in cell adhesion/migration. It becomes clear that the subcellular localization of Src and FAK is crucial for their activities and functions. In this article, we first overview the molecular mechanisms and functions of Src and FAK involved in cell adhesion/migration.
Jihye, Seong, Shaoying, Lu, Yingxiao, Wang   +2 more
openaire   +2 more sources