Results 1 to 10 of about 1,105,287 (296)
An RNase domain-dependent microRNA detection with DNA-spiked nanocage for accurate cancer diagnosis [PDF]
Cancer-associated microRNAs are promising biomarkers for early cancer diagnosis. However, current microRNA detections – which are based on polymerase chain reaction, microarray analysis, Raman scattering, or various electrochemical assays – rely on multi-
Hye Hyun Kim +4 more
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Protocol to quantify desmoglein 3 tension at cell-cell adhesions using FRET-based tension sensors [PDF]
Summary: Desmoglein 3 (Dsg3) is a crucial component of cell-cell junctions, contributing to mechanotransduction and maintaining tissue integrity. Here, we present a protocol to quantify tension at keratinocyte cell-cell adhesion sites using a Dsg3 ...
Bahareh Tajvidi Safa +5 more
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Measuring Förster–resonance–energy–transfer (FRET) efficiency allows the investigation of protein–protein interactions (PPI), but extracting quantitative measures of affinity necessitates highly advanced technical equipment or isolated proteins.
Bernhard Hochreiter +6 more
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Supramolecular nanostructured materials, displaying Förster resonance energy transfers (FRET) signals, have become the focus of interest for many researchers across the globe.
Amir Sohail
doaj +5 more sources
CRX and NRL are retina-specific transcription factors that control rod photoreceptor differentiation and synergistically activate rod phototransduction gene expression.
Xinming Zhuo, Barry E. Knox
doaj +1 more source
In vivo imaging of hierarchical spatiotemporal activation of caspase-8 during apoptosis. [PDF]
BACKGROUND: Activation of caspases is crucial for the execution of apoptosis. Although the caspase cascade associated with activation of the initiator caspase-8 (CASP8) has been investigated in molecular and biochemical detail, the dynamics of CASP8 ...
Katsuya Kominami +15 more
doaj +1 more source
Nanoscale Optical Logic Circuits by Single-Step FRET
We propose a method for implementing nanoscale optical logic circuits via single-step Förster resonance energy transfer (FRET). Use of single-step FRET enabled circuits with simple design and high expandability. Two kinds of FRET gates, a YES gate
Jinya Inoue +3 more
doaj +1 more source
Fluorescence polarization and fluctuation analysis monitors subunit proximity, stoichiometry, and protein complex hydrodynamics. [PDF]
Förster resonance energy transfer (FRET) microscopy is frequently used to study protein interactions and conformational changes in living cells. The utility of FRET is limited by false positive and negative signals.
Tuan A Nguyen +4 more
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Three-Dimensional Reconstruction of Three-Way FRET Microscopy Improves Imaging of Multiple Protein-Protein Interactions. [PDF]
Fluorescence resonance energy transfer (FRET) microscopy is a powerful tool for imaging the interactions between fluorescently tagged proteins in two-dimensions. For FRET microscopy to reach its full potential, it must be able to image more than one pair
Brandon L Scott, Adam D Hoppe
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Protein–protein interactions play pivotal roles in life, and the protein interaction affinity confers specific protein interaction events in physiology or pathology.
Jiayu Liao +3 more
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