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Production of 3-Fucosyllactose in Engineered Escherichia coli with α-1,3-Fucosyltransferase from Helicobacter pylori.

Biotechnology Journal, 2019
3-Fucosyllactose (3-FL), one of the major oligosaccharides in human breast milk, is produced in engineered Escherichia coli. In order to search for a good α-1,3-fucosyltransferase, three bacterial α-1,3-fucosyltransferases are expressed in engineered E ...
Sang-Min Jung   +2 more
semanticscholar   +1 more source

Substrate Characterization of Bacteroides fragilis α1,3/4-Fucosyltransferase Enabling Access to Programmable One-Pot Enzymatic Synthesis of KH-1 Antigen

, 2019
Bacteroides fragilis α1,3/4-fucosyltransferase (Bf13FT) was expressed in Escherichia coli and characterized as an α1,3/4-fucosyltransferase that can be used as a versatile catalyst for the synthesi...
Hsin-Hui Huang   +9 more
semanticscholar   +1 more source

Characterization of fucosyltransferase activity during mouse spermatogenesis: evidence for a cell surface fucosyltransferase

Biochemistry, 1989
Fucosyltransferase activity was quantified in mouse germ cells at different stages of spermatogenesis. Specifically, fucosyltransferase activities of pachytene spermatocytes, round spermatids, and cauda epididymal sperm were compared. Fucosyltransferase activity of mixed germ cells displayed an apparent Vmax of 17 pmol (mg of protein)-1 min-1 and an ...
R A, Cardullo   +2 more
openaire   +2 more sources

α6-Fucosyltransferase (FUT8)

2002
GDP-L-FucN-acetyl-β-D-glucosaminide α1-6fucosyltransferase (FUT8) catalyzes the transfer of fucose from GDP-Fuc to N-linked-type complex glycoproteins, as shown in Fig. 1. The enzymatic products, α1,6-fucosylated (core fucosylated) N-glycans, are commonly observed in many glycoproteins, and are especially abundant in brain tissue. It is well known that
Eiji Miyoshi, Naoyuki Taniguchi
openaire   +1 more source

Structural basis for Lewis antigen synthesis by the α1,3-fucosyltransferase FUT9

Nature Chemical Biology, 2023
R. Kadirvelraj   +11 more
semanticscholar   +1 more source

Evaluation of serum fucosyltransferases in malignancy

European Journal of Cancer and Clinical Oncology, 1984
Incorporation of [14C]-fucose into the endogenous acceptor of serum and into the exogenous acceptors desialofetuin (alpha 2-fucosyltransferase) and desialodegalactofetuin (alpha 3-fucosyltransferase) was determined in controls and in patients with various malignant diseases.
R, Kuzmits, P, Aiginger
openaire   +2 more sources

Fucosyltransferase 8 plays a crucial role in the invasion and metastasis of pancreatic ductal adenocarcinoma

Surgery today (Print), 2020
Kazuhiro Tada   +13 more
semanticscholar   +1 more source

α3-Fucosyltransferase-V (FUT5)

2002
Fuc-TVI (FUT6) was cloned as a fourth member of the human α1→3/4 fucosyltransferase gene family by Weston et al. in 1992. The enzyme shared 85% and 89% amino acid sequence identity with previously cloned Fuc-TIII (FUT3) and Fuc-TV (FUT5), respectively, and the FUT6 gene encoding the enzyme was shown to be located on chromosome 19, where the genes for ...
openaire   +1 more source

α3-Fucosyltransferase-IV (FUT4)

2002
To date, six human α3-fucosyltransferases (α1,3-FUTs) have been cloned. α3- fucosyltransferase-IX (Fuc-TIX, FUT9) is the newest member of the six human α3- FUTs, and has been cloned by an expression cloning method from a mouse brain cDNA library (Kudo et al. 1998). All the α1,3-FUT members, including those of Helicobacter pylori (Ge et al.
openaire   +1 more source

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