Results 121 to 130 of about 57,547 (250)

Accessibility and Alignment of Trans‐Cleavage Substrates Modulate the Kinetics of Cas12a Ribonucleoprotein

open access: yesAngewandte Chemie, EarlyView.
Two key binding events that collectively determine the catalytic efficiency of trans‐cleavage: (1) the substrate passing through the gap between the REC and NUC domains, determined by Kpass, and (2) the alignment of the substrate with the catalytic triad of the ribonucleoprotein, determined by Kalign.
Jianyu Hu   +3 more
wiley   +2 more sources

dsRADAR: Imaging and Detecting Cellular dsRNA by Repurposing RNA‐Binding Proteins

open access: yesAngewandte Chemie, EarlyView.
Double‐stranded RNA (dsRNA) plays an important role in the innate immune system, but can be misregulated in disease, leading to inflammation. Despite the importance of this motif, methods for detecting dsRNA remain limited. This work shows that ADAR3 can be repurposed to provide improved detection of dsRNA, which is demonstrated in the context of viral
Weina Cheng   +7 more
wiley   +2 more sources

RNA Organelles in DNA‐Based Artificial Cells Provide Spatial Aptamer Functions and Enhanced Signal Processing

open access: yesAngewandte Chemie, EarlyView.
Multiphase DNA–RNA artificial cells integrate a protective DNA shell, a transcriptionally active DNA core, and a functional RNA organelle. Spatial compartmentalization enables signal generation, external protein expression, and selective recapture via RNA aptamers. ABSTRACT Naturally occurring biomolecular condensates orchestrate key cellular processes
Maria Vonk‐de Roy   +4 more
wiley   +2 more sources

Aktivierung von Metallkomplexen durch Ultraschallwellen für eine gezielte Krebstherapie

open access: yesAngewandte Chemie, EarlyView.
Ultrasound enables non‐invasive activation of metal‐based therapeutics deep within tumors. Acoustic cavitation can drive sonodynamic reactive oxygen species generation or mechanochemical metal–ligand bond dissociation, enabling controlled release of bioactive payloads.
Félix Grosjean   +3 more
wiley   +1 more source

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