Results 161 to 170 of about 373,199 (312)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Approximation Performance Analysis and Application of F Characteristics in Fractance Approximation Circuit

open access: yes四川大学学报. 自然科学版, 2018
F-frequency characteristics function is a new concept about lumped eigenvalue of the fractance approximation circuit. In order to quantitatively describe the approximation performance of F-frequency characteristics of the fractance approximation circuit,
YU Bo   +3 more
doaj  

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

On Approximation by Nonvanishing Functions [PDF]

open access: yesProceedings of the American Mathematical Society, 1959
openaire   +2 more sources

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

Theory I: Deep networks and the curse of dimensionality [PDF]

open access: yesBulletin of the Polish Academy of Sciences: Technical Sciences, 2018
T. Poggio, Q. Liao
doaj   +1 more source

Improved initial approximation for errors-in-variables system identification

open access: yes
Errors-in-variables system identification can be posed and solved as a Hankel structured low-rank approximation problem. In this paper different estimates based on suboptimal low-rank approximations are considered.
Usevich, Konstantin
core  

Asymptotic Solutions of the Phase Space Schrodinger Equation: Anisotropic Gaussian Approximation

open access: yes, 2014
We consider the singular semiclassical initial value problem for the phase space Schrodinger equation. We approximate semiclassical quantum evolution in phase space by analyzing initial states as superpositions of Gaussian wave packets and applying ...
Karageorge, Panos D., Makrakis, G.N.
core  

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

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