Results 31 to 40 of about 400,284 (267)

pTripleTREP – A vector for tightly controlled expression and purification of virulence factors in Staphylococcus aureus

Microbial Cell Factories
Background Recombinant proteins facilitate and contribute to detailed studies of the virulence mechanisms and pathophysiology of the major human pathogen Staphylococcus aureus. Of particular interest are secreted virulence factors.
Hannes Wolfgramm, Christopher Saade, Marco Harms, Larissa M. Busch, Josephine Lange, Maximilian Schedlowski, Kristin Surmann, Manuela Gesell Salazar, Christian Hentschker, Leif Steil, Stephan Michalik, Uwe Völker, Alexander Reder   +12 more
doaj   +1 more source

Trypanosoma cruzi specific mRNA amplification by in vitro transcription improves parasite transcriptomics in host-parasite RNA mixtures

BMC Genomics, 2017
Background Trypanosomatids are a group of protozoan parasites that includes the etiologic agents of important human illnesses as Chagas disease, sleeping sickness and leishmaniasis.
Rafael Luis Kessler, Daniela Parada Pavoni, Marco Aurelio Krieger, Christian Macagnan Probst   +3 more
doaj   +1 more source

The GWAS-MAP platform for aggregation of results of genome-wide association studies and the GWAS-MAP|homo database of 70 billion genetic associations of human traits

Вавиловский журнал генетики и селекции, 2020
Hundreds of genome-wide association studies (GWAS) of human traits are performed each year. The results of GWAS are often published in the form of summary statistics.
T. I. Shashkova, D. D. Gorev, E. D. Pakhomov, A. S. Shadrina, S. Zh. Sharapov, Y. A. Tsepilov, L. C. Karssen, Y. S. Aulchenko   +7 more
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Global quantitative proteome analysis of a multi-resistant Klebsiella pneumoniae strain

Frontiers in Microbiology
Klebsiella pneumoniae is a critical nosocomial pathogen with a rising incidence in antibiotic resistance worldwide. Multidrug-resistant strains of K. pneumoniae pose a major health threat, particularly to immunosuppressed and elderly patients.
Marie-Sofie Illenseher, Marie-Sofie Illenseher, Christian Hentschker, Manuela Gesell Salazar, Larissa Milena Busch, Lisa Zierke, Alexander Reder, Stephan Michalik, Uwe Völker, Sven Hammerschmidt, Kristin Surmann   +10 more
doaj   +1 more source

Proteome data of whole saliva which are associated with development of oral mucositis in head and neck cancer patients undergoing radiotherapy

Data in Brief, 2016
Saliva as major human body fluid may act as an indicator of oral disease status. Oral mucositis is a common and often treatment-limiting side effect of radiotherapy for head and neck cancer patients.
Nico Jehmlich, Petra Stegmaier, Claas Golatowski, Manuela Gesell Salazar, Christian Rischke, Michael Henke, Uwe Völker   +6 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Genomics and Functional Genomics of Malignant Pleural Mesothelioma [PDF]

International Journal of Molecular Sciences, 2020
Malignant pleural mesothelioma (MPM) is a rare, aggressive cancer of the mesothelial cells lining the pleural surface of the chest wall and lung. The etiology of MPM is strongly associated with prior exposure to asbestos fibers, and the median survival rate of the diagnosed patients is approximately one year. Despite the latest advancements in surgical
Ece Cakiroglu, Serif Senturk
openaire   +4 more sources

Unraveling proteomic chaos by independent component analysis—ClpX proficiency promotes the iron and oxygen limitation responses of Staphylococcus aureus and affects the intracellular bacterial behavior

mSystems
In the opportunistic pathogen Staphylococcus aureus, protein homeostasis is largely mediated by the caseinolytic protease (Clp) system. The proteases ClpXP and ClpCP are crucial for general and targeted proteolysis, which rely on the unfoldases ClpX and ...
Larissa M. Busch, Hannes Wolfgramm, Supradipta De, Christian Hentschker, Manuela Gesell Salazar, Meike Kröber, Celina Hopp, Marie-Sofie Illenseher, Alexander Ganske, Stephan Michalik, Alexander Reder, Sven Hammerschmidt, Dorte Frees, Ulf Gerth, Kristin Surmann, Ulrike Mäder, Uwe Völker   +16 more
doaj   +1 more source