Results 191 to 200 of about 68,679 (255)
Development of human monoclonal antibodies against TARM1 by yeast display
FEBS Open Bio, EarlyView.Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe +5 more
wiley +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
FEBS Open Bio, EarlyView.14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa +2 more
wiley +1 more source
FEBS Open Bio, EarlyView.This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Perspectives on egressibility of older people with functional limitations
Fire Safety Journal, 2022 Older people and people with functional limitations are recognized as vulnerable groups when it comes to evacuation. Previous studies have focused mostly on the quantitative aspects of their physical characteristics, such as movement speeds.
Erik Smedberg +2 more
exaly +2 more sources
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2021
Let \(\mathbb{R}\) denote the real line, and let \(E\) be the Banach space of bounded real-valued functions with the norm \(|f|=\text{ess sup}\{|f(x)|, x\in\mathbb{R}\}\). \(L\) denotes the set of locally integrable functions. For \(a>0\), the transform \(T_a(f)\) is defined by \[ T_a(f)(x)= (1/a) \int^{x+a}_x f(t)dt,\quad a>0,\quad T_0(f)(x)= f(x). \]
Das, G., Nanda, S.
openaire +2 more sources
Let \(\mathbb{R}\) denote the real line, and let \(E\) be the Banach space of bounded real-valued functions with the norm \(|f|=\text{ess sup}\{|f(x)|, x\in\mathbb{R}\}\). \(L\) denotes the set of locally integrable functions. For \(a>0\), the transform \(T_a(f)\) is defined by \[ T_a(f)(x)= (1/a) \int^{x+a}_x f(t)dt,\quad a>0,\quad T_0(f)(x)= f(x). \]
Das, G., Nanda, S.
openaire +2 more sources
Limits of Liouvillian Functions
Proceedings of the London Mathematical Society, 1996zbMATH Open Web Interface contents unavailable due to conflicting licenses.
openaire +2 more sources
2009
There are several reasonable definitions for the limit of a sequence of functions. Clearly the entries of the sequence should approximate the limit function f to greater and greater accuracy in some sense. But there are different ways of measuring the accuracy of an approximation, depending on the problem.
Kenneth R. Davidson, Allan P. Donsig
+4 more sources
There are several reasonable definitions for the limit of a sequence of functions. Clearly the entries of the sequence should approximate the limit function f to greater and greater accuracy in some sense. But there are different ways of measuring the accuracy of an approximation, depending on the problem.
Kenneth R. Davidson, Allan P. Donsig
+4 more sources