SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Comparative analysis of the relationship of sarcopenia or sarcopenic obesity with functional impairment: A cross-sectional study. [PDF]
Gomes FA +6 more
europepmc +1 more source
Naked cuticle is essential for Drosophila wing development beyond Wingless signaling
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley +1 more source
Sustainable Augmentation of Chickpea Protein Functionality and In Vitro Digestion via Spent Coffee Phenolics: A Protein-Phenolic Interaction Study. [PDF]
Saricaoglu B +2 more
europepmc +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
Reinfection-Driven Accumulation of SARS-CoV-2 Antibodies: A 36-Month Longitudinal Study in Austrian Blood Donors. [PDF]
Kartal O +12 more
europepmc +1 more source
The short-term effectiveness of antidepressants in a transdiagnostic sample and determinants of side effects and medication nonadherence: an observational study. [PDF]
Bağcaz A, Özel B.
europepmc +1 more source
Treating chronic pain with low dose ketamine and adjunct therapies within a biopsychosocial approach: a case series. [PDF]
Almog S +5 more
europepmc +1 more source
The influence of sarcopenia on health-related quality of life among older patients with gastrointestinal cancer: an assessment using the SarQoL<sup>®</sup> questionnaire. [PDF]
Kamińska A +4 more
europepmc +1 more source

