Results 11 to 20 of about 211,518 (253)
Comparison of methods for isolating fungal DNA [PDF]
Czech Journal of Food Sciences, 2011 In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as most detection techniques require DNA amplification ...
Petra MOŤKOVÁ, Jarmila VYTŘASOVÁ
doaj +2 more sources
Influence of DNA extraction kits on freshwater fungal DNA metabarcoding. [PDF]
PeerJ, 2022 Abstract Background Environmental DNA (eDNA) metabarcoding is a common technique for efficient biodiversity monitoring, especially of microbes. Recently, the usefulness of aquatic eDNA in monitoring the diversity of both terrestrial and aquatic fungi has been suggested.
Matsuoka S, Sugiyama Y, Nagano M, Doi H.
europepmc +5 more sources
Unraveling the ancient fungal DNA from the Iceman gut. [PDF]
BMC GenomicsAbstract Here, we explore the possible ancient fungal species in the gut of Ötzi, the Iceman, a naturally mummified human found in the Tyrolean Alps (border between Italy and Austria). While ancient DNA (aDNA) has been extensively used to study human, animal, and plant evolution, this research focuses on ancient ...
Oskolkov N +8 more
europepmc +8 more sources
Fungal DNA in Circulation: A Sleuth's Clue to Predict Cardiovascular Risk for Hemodialysis Patients. [PDF]
J Am Soc NephrolJain N +3 more
europepmc +3 more sources
The Quest for a General and Reliable Fungal DNA Barcode [PDF]
The Open Applied Informatics Journal, 2011 DNA sequences are key elements for both identification and classification of living organisms. Mainly for historical reasons, a limited number of genes are currently used for this purpose. From a mathematical point of view, any DNA segment, at any location, even outside of coding regions and even if they do not align, could be used as long as PCR ...
V. Robert +7 more
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Rapid Mini-Preparation of Fungal DNA for PCR [PDF]
Journal of Clinical Microbiology, 2000 Nucleic acid detection methods such as PCR have become a common tool for microbial identification and diagnosis. Although PCR amplification can be performed directly for various microbial cultures, for filamentous fungi and yeasts, prior isolation of DNA is often preferred.
D, Liu, S, Coloe, R, Baird, J, Pederson
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Detailed Protocol to Perform Direct PCR Using Filamentous Fungal Biomass—Tips and Considerations
Bio-Protocol, 2023 The precise and rapid detection of fungi is important in various fields, including clinics, industry, and agriculture. While sequencing universal DNA barcodes remains the standard method for species identification and phylogenetic analysis, a significant
Hosung Jeon, Hokyoung Son, Kyunghun Min
doaj +1 more source
A simple method for extraction of fungal genomic DNA [PDF]
Letters in Applied Microbiology, 2000 We have developed a new, simple and effective method for extraction of fungal genomic DNA. The initial steps involved suspension of freeze-dried mycelium in buffer containing sodium dodecyl sulphate, detachment of DNA from polysaccharides by mild shearing, NaCl precipitation of polysaccharides and protein, chloroform extraction and ethanol ...
T H, Al-Samarrai, J, Schmid
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Candida glabrata: A Lot More Than Meets the Eye
Microorganisms, 2019 Candida glabrata is an opportunistic human fungal pathogen that causes superficial mucosal and life-threatening bloodstream infections in individuals with a compromised immune system. Evolutionarily, it is closer to the non-pathogenic yeast Saccharomyces
Kundan Kumar +3 more
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Use of magnetic beads to extract fungal DNA [PDF]
Mycoses, 2005 SummaryAuthors compare two methods of extracting DNA from different fungi: the classic method with phenol/chloroform (P/C) and that with magnetic beads. Both were tested on Candida albicans and Cryptococcus neoformans var. neoformans, belonging to the yeast group and Microsporum canis, M. gypseum, Trichophyton rubrum, T. interdigitale, T.
FAGGI, ELISABETTA +2 more
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