Comparison of methods for isolating fungal DNA [PDF]
Practical Laboratory Medicine, 2021 Objectives: The main aim of this work was to compare the methods of DNA isolation in the moulds of genus Mucorales with special regard to the amount and purity of the DNA acquired. The acquired DNA was then amplified by specific real-time PCR.
Ilaria Galliano +7 more
doaj +6 more sources
Diversity of Fungal DNA Methyltransferases and Their Association With DNA Methylation Patterns [PDF]
Frontiers in Microbiology, 2021 DNA methyltransferases (DNMTs) are a group of proteins that catalyze DNA methylation by transferring a methyl group to DNA. The genetic variation in DNMTs results in differential DNA methylation patterns associated with various biological processes.
Yu-Shin Nai +6 more
doaj +5 more sources
The Pattern and Function of DNA Methylation in Fungal Plant Pathogens [PDF]
Microorganisms, 2020 To successfully infect plants and trigger disease, fungal plant pathogens use various strategies that are dependent on characteristics of their biology and genomes.
Chang He +3 more
doaj +5 more sources
Influence of DNA extraction kits on freshwater fungal DNA metabarcoding. [PDF]
PeerJ, 2022 Abstract Background Environmental DNA (eDNA) metabarcoding is a common technique for efficient biodiversity monitoring, especially of microbes. Recently, the usefulness of aquatic eDNA in monitoring the diversity of both terrestrial and aquatic fungi has been suggested.
Matsuoka S, Sugiyama Y, Nagano M, Doi H.
europepmc +5 more sources
Optimizing fungal DNA extraction and purification for Oxford Nanopore untargeted shotgun metagenomic sequencing from simulated hemoculture specimens [PDF]
mSystemsLong-read metagenomics provides a promising alternative approach to fungal identification, circumventing methodological biases, associated with DNA amplification, which is a prerequisite for DNA barcoding/metabarcoding based on the primary fungal DNA ...
Nattapong Langsiri +8 more
doaj +2 more sources
Comparison of methods for isolating fungal DNA [PDF]
Czech Journal of Food Sciences, 2011 In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as most detection techniques require DNA amplification ...
Petra MOŤKOVÁ, Jarmila VYTŘASOVÁ
doaj +2 more sources
Unraveling the ancient fungal DNA from the Iceman gut. [PDF]
BMC GenomicsAbstract Here, we explore the possible ancient fungal species in the gut of Ötzi, the Iceman, a naturally mummified human found in the Tyrolean Alps (border between Italy and Austria). While ancient DNA (aDNA) has been extensively used to study human, animal, and plant evolution, this research focuses on ancient ...
Oskolkov N +8 more
europepmc +7 more sources
Introduction of a bead beating step improves fungal DNA extraction from selected patient specimens
International Journal of Medical Microbiology, 2020 In immunocompromised patients a colonisation with fungi carries the risk to develop serious invasive fungal infection. An early detection is therefore important, but not optimal hitherto.
Sebastian Scharf +5 more
doaj +3 more sources
mBio, 2021 Although relatively few in number, studies of DNA methylation have shown that fungal DNA methylation is implicated in development, genome integrity, and genome defense.
Yo-Han Ko +3 more
doaj +3 more sources
Optimizing a modified cetyltrimethylammonium bromide protocol for fungal DNA extraction: Insights from multilocus gene amplification [PDF]
Open Life SciencesGenomic DNA (gDNA) extraction is an important step in many molecular studies of fungal biology, and it is necessary to evaluate the efficiency, cost-effectiveness, and efficacy of different extraction methods to ensure successful amplification of the ...
Dar Gulam Jeelani +5 more
doaj +2 more sources