Results 21 to 30 of about 201,219 (249)
The Impact of DNA Extraction Methods on Stool Bacterial and Fungal Microbiota Community Recovery
Frontiers in Microbiology, 2019 Our understanding of human gut microbiota in health and disease depends on accurate and reproducible microbial data acquisition. The critical step in this process is to apply an appropriate methodology to extract microbial DNA, since biases introduced ...
Kristýna Fiedorová +12 more
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Evaluating the Impact of DNA Extraction Method on the Representation of Human Oral Bacterial and Fungal Communities. [PDF]
PLoS ONE, 2017 The application of high-throughput, next-generation sequencing technologies has greatly improved our understanding of the human oral microbiome. While deciphering this diverse microbial community using such approaches is more accurate than traditional ...
Anna Vesty +4 more
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Epigenetic Regulation of Antifungal Drug Resistance
Journal of Fungi, 2022 In medical mycology, epigenetic mechanisms are emerging as key regulators of multiple aspects of fungal biology ranging from development, phenotypic and morphological plasticity to antifungal drug resistance.
Sandip Patra +3 more
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Bio-Protocol, 2021 Flavodoxin-like proteins (Fld-LPs) are an important constituent of the oxidative stress defense system in several organisms and highly conserved from bacteria to humans.
Anamika Battu +2 more
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Phosphatidylinositol 3-phosphate regulates iron transport via PI3P-binding CgPil1 protein
Cell Reports, 2023 Summary: Iron homeostasis, which is pivotal to virulence, is regulated by the phosphatidylinositol 3-kinase CgVps34 in the human fungal pathogen Candida glabrata.
Fizza Askari +2 more
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SUMOylation in Human Pathogenic Fungi: Role in Physiology and Virulence
Journal of Fungi, 2020 The small ubiquitin-related modifier (SUMO) protein is an important component of the post-translational protein modification systems in eukaryotic cells.
Mahima Sagar Sahu +3 more
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The Quest for a General and Reliable Fungal DNA Barcode [PDF]
The Open Applied Informatics Journal, 2011 DNA sequences are key elements for both identification and classification of living organisms. Mainly for historical reasons, a limited number of genes are currently used for this purpose. From a mathematical point of view, any DNA segment, at any location, even outside of coding regions and even if they do not align, could be used as long as PCR ...
V. Robert +7 more
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Detailed Protocol to Perform Direct PCR Using Filamentous Fungal Biomass—Tips and Considerations
Bio-Protocol, 2023 The precise and rapid detection of fungi is important in various fields, including clinics, industry, and agriculture. While sequencing universal DNA barcodes remains the standard method for species identification and phylogenetic analysis, a significant
Hosung Jeon, Hokyoung Son, Kyunghun Min
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Rapid Mini-Preparation of Fungal DNA for PCR [PDF]
Journal of Clinical Microbiology, 2000 Nucleic acid detection methods such as PCR have become a common tool for microbial identification and diagnosis. Although PCR amplification can be performed directly for various microbial cultures, for filamentous fungi and yeasts, prior isolation of DNA is often preferred.
D, Liu, S, Coloe, R, Baird, J, Pederson
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A simple method for extraction of fungal genomic DNA [PDF]
Letters in Applied Microbiology, 2000 We have developed a new, simple and effective method for extraction of fungal genomic DNA. The initial steps involved suspension of freeze-dried mycelium in buffer containing sodium dodecyl sulphate, detachment of DNA from polysaccharides by mild shearing, NaCl precipitation of polysaccharides and protein, chloroform extraction and ethanol ...
T H, Al-Samarrai, J, Schmid
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