Results 21 to 30 of about 1,916,984 (295)

A simple method for extraction of fungal genomic DNA [PDF]

Letters in Applied Microbiology, 2000
We have developed a new, simple and effective method for extraction of fungal genomic DNA. The initial steps involved suspension of freeze-dried mycelium in buffer containing sodium dodecyl sulphate, detachment of DNA from polysaccharides by mild shearing, NaCl precipitation of polysaccharides and protein, chloroform extraction and ethanol ...
T H, Al-Samarrai, J, Schmid
openaire   +2 more sources

An efficient method for DNA extraction from Cladosporioid fungi [PDF]

, 2010
We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Bahkali, A.H., Abd-Elsalam, K.A., Wit, P.J.G.M., de, Moslem, M.A.   +3 more
core   +1 more source

Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation [PDF]

, 2019
Species identification lies at the heart of biodiversity studies that has in recent years favoured DNA-based approaches. Microbial Biological Resource Centres are a rich source for diverse and high-quality reference materials in microbiology, and yet the
Vries, M., de, de Vries, M, Boekhout, T, A. Al-Hatmi, D. Vu, Groenewald, J.Z., Groenewald, Johannes Zacharias, Gehrmann, T, B. Stielow, de Vries, M., Al-Hatmi, A, Crous, Pedro W., Verkley, G.J.M., Robert, V., Eberhardt, U, G.J.M. Verkley, V. Robert, Houbraken, J, Vu, D, Groenewald, Marizeth, T. Boekhout, J.Z. Groenewald, T. Gehrmann, Gehrmann, T., Al-Hatmi, A., Crous, P.W., Houbraken, J., Eberhardt, U., M. Groenewald, Boekhout, T., U. Eberhardt, Stielow, B., Stielow, B, Robert, Vincent, P.W. Crous, M. de Vries, Vu, D., Groenewald, M., Cardinali, G., G. Cardinali, J. Houbraken   +40 more
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Identification of Contaminating Fungal DNA Sequences in Zymolyase [PDF]

Journal of Clinical Microbiology, 1999
ABSTRACT When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu ...
D, Rimek, A P, Garg, W H, Haas, R, Kappe
openaire   +2 more sources

RIPCAL: a tool for alignment-based analysis of repeat-induced point mutations in fungal genomic sequences [PDF]

, 2008
Background Repeat-induced point mutation (RIP) is a fungal-specific genome defence mechanism that alters the sequences of repetitive DNA, thereby inactivating coding genes.
Hane, J., Oliver, Richard, James K Hane, Richard P Oliver, Oliver, R.P., Hane James K, Hane, J.K., Oliver Richard P   +7 more
core   +1 more source

Mycelial DNA persistence in a forest soil

Environmental DNA, 2021
Living fungi can be identified and monitored in their soil habitat using an array of molecular methods. However, DNA originating from dead fungal tissue may become stabilized in the soil and remain detectable for some time after cell death rendering ...
Matthew Gordon, Kelli Van Norman
doaj   +1 more source

Host genotype shapes the foliar fungal microbiome of balsam poplar (Populus balsamifera) [PDF]

, 2013
Foliar fungal communities of plants are diverse and ubiquitous. In grasses endophytes may increase host fitness; in trees, their ecological roles are poorly understood.
Fankhauser, Johnathon D., Johnathon D Fankhauser, Johnathon D. Fankhauser (104143), Matthew S Olson, Miklós Bálint (104125), Schmitt, Imke, Olson, Matthew S., Imke Schmitt (104151), Tiffin, Peter, Meike Piepenbring, Hallström, Björn, Matthew S. Olson (104138), Peter Tiffin, Robert B. O’Hara (104134), Peter Tiffin (104127), Björn Hallström (104129), Bálint, Miklós, Imke Schmitt, Miklós Bálint, Robert B O'Hara, O'Hara, Robert B., Piepenbring, Meike, Björn Hallström, Meike Piepenbring (104147)   +23 more
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Fungal DNA Extraction from Fungal Plate v1

, 2020
This protocol describes how to do fungal DNA extraction from fungal plate. This protocol is part of the Bark Beetle Mycobiome (BBM) Research Coordination Network. For more information on the BBM international network: Hulcr J, Barnes I, De Beer ZW, Duong TA, Gazis R, Johnson AJ, Jusino MA, Kasson MT, Li Y, Lynch S, Mayers C, Musvuugwa T, Roets F ...
You Li, Demian F Gomez
openaire   +1 more source

Evolutionary Relationship and the Sequence Similarities among Different Fungal Species Infecting Birds Captured from Different Areas in Denmark [PDF]

Archives of Razi Institute, 2022
Fungal diseases are the common cause of death in wild animals and birds of prey. This study was designed to investigate the development of fungal infections among wild birds in Denmark.
Z Shaker Al-Rubaiee, M Salh Hussin, S Baho   +2 more
doaj   +1 more source

DNA quantification of basidiomycetous fungi during storage of logging residues [PDF]

PeerJ, 2015
The demand for bioenergy caused an increased use of logging residues, branches and treetops that were previously left on the ground after harvesting. Residues are stored outdoors in piles and it is unclear to what extent fungi transform this material ...
Isabella Børja, Gry Alfredsen, Tore Filbakk, Carl Gunnar Fossdal   +3 more
doaj   +2 more sources