Results 21 to 30 of about 211,518 (253)

Identification of Contaminating Fungal DNA Sequences in Zymolyase [PDF]

open access: yesJournal of Clinical Microbiology, 1999
ABSTRACT When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu ...
D, Rimek, A P, Garg, W H, Haas, R, Kappe
openaire   +2 more sources

Distinct Roles of Two DNA Methyltransferases from Cryphonectria parasitica in Fungal Virulence, Responses to Hypovirus Infection, and Viral Clearance

open access: yesmBio, 2021
Although relatively few in number, studies of DNA methylation have shown that fungal DNA methylation is implicated in development, genome integrity, and genome defense.
Yo-Han Ko   +3 more
doaj   +1 more source

Fungal DNA Extraction from Fungal Plate v1

open access: yes, 2020
This protocol describes how to do fungal DNA extraction from fungal plate. This protocol is part of the Bark Beetle Mycobiome (BBM) Research Coordination Network. For more information on the BBM international network: Hulcr J, Barnes I, De Beer ZW, Duong TA, Gazis R, Johnson AJ, Jusino MA, Kasson MT, Li Y, Lynch S, Mayers C, Musvuugwa T, Roets F ...
You Li, Demian F Gomez
openaire   +1 more source

Introduction of a bead beating step improves fungal DNA extraction from selected patient specimens

open access: yesInternational Journal of Medical Microbiology, 2020
In immunocompromised patients a colonisation with fungi carries the risk to develop serious invasive fungal infection. An early detection is therefore important, but not optimal hitherto.
Sebastian Scharf   +5 more
doaj   +1 more source

Tracking fungal community responses to maize plants by DNA- and RNA-based pyrosequencing. [PDF]

open access: yesPLoS ONE, 2013
We assessed soil fungal diversity and community structure at two sampling times (t1 = 47 days and t2 = 104 days of plant age) in pots associated with four maize cultivars, including two genetically modified (GM) cultivars by high-throughput ...
Eiko E Kuramae   +6 more
doaj   +1 more source

Molecular Characterization of Subunit G of the Vacuolar ATPase in Pathogen Dermatophyte Trichophyton rubrum [PDF]

open access: yesIranian Journal of Public Health, 2006
Trichophyton rubrum is an anthropophilic fungus causing up to 90% of chronic cases of dermatophytosis. Several properties of this fungus have been investigated so far.
S Rezaie   +3 more
doaj   +1 more source

Mycelial DNA persistence in a forest soil

open access: yesEnvironmental DNA, 2021
Living fungi can be identified and monitored in their soil habitat using an array of molecular methods. However, DNA originating from dead fungal tissue may become stabilized in the soil and remain detectable for some time after cell death rendering ...
Matthew Gordon, Kelli Van Norman
doaj   +1 more source

General Fungal DNA Extraction v2

open access: yes, 2023
This is a routine protocol for extracting DNA from various fungi. This extraction method is suitable for follow-up molecular work such as PCR amplification.
Angie Macias   +2 more
openaire   +1 more source

DNA quantification of basidiomycetous fungi during storage of logging residues [PDF]

open access: yesPeerJ, 2015
The demand for bioenergy caused an increased use of logging residues, branches and treetops that were previously left on the ground after harvesting. Residues are stored outdoors in piles and it is unclear to what extent fungi transform this material ...
Isabella Børja   +3 more
doaj   +2 more sources

Combined bacterial and fungal targeted amplicon sequencing of respiratory samples: Does the DNA extraction method matter?

open access: yesPLoS ONE, 2020
BACKGROUND:High-throughput sequencing techniques are used to analyse the diversity of the respiratory microbiota in health and disease. Although extensive data are available regarding bacterial respiratory microbiota, its fungal component remains poorly ...
Cécile Angebault   +4 more
doaj   +1 more source

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