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In DNA methylation, methyl groups are covalently bound to CpG dinucleotides. However, the assumption that methyl groups are not lost during routine DNA extraction has not been empirically tested.
Carolina Soriano-Tárraga +11 more
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A simple and cost-effective method for DNA extraction suitable for PCR in “sucupira branca”
“Sucupira branca” is a plant found in the Brazilian Cerrado and is adapted to low fertility soils, and its fruit extract has anti-inflammatory, healing, antiulcerogenic, antimicrobial, cercaricidal, leishmanicidal and antioxidant activities. Furthermore,
Jaílson do Nascimento Silva +6 more
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Optimized method for dna extraction and PCR amplification in aroeira tree
Molecular markers are important tools in the characterization of plant genetic diversity and can provide support for conservation strategies for endangered populations.
Jailson do Nascimento Silva +8 more
doaj +1 more source
The features of diagnostics of grain crop seeds intended for export
Russia occupies a leading position among the largest wheat producers and exporters. One of the key requirements for the biological safety of domestic grain products is the phytosanitary requirements of importing countries.
E. R. Ruchkov +3 more
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Comparative analysis of reagent kits for DNA extraction from dry blood stains
Neonatal screening is a mandatory newborn screening procedure that detects the presence of genetic diseases. Dry blood stains are used for mass screening of children.
A. V. Sedykh +3 more
doaj +1 more source
Gelatin-coated magnetic nanoparticles-based DNA isolation method: A comparison with commercial DNA isolation kits from whole blood [PDF]
DNA isolation is the first step for most molecular tests, thus indicating the importance of using a reliable and suitable method for this procedure. The use of magnetic nanoparticles for blood DNA isolation offers several advantages including subjecting ...
Kelly Levano +3 more
doaj +1 more source
Comparison of DNA isolation methods from mammalian sperm cells and development of a new protocol
Sperm DNA tightly packed with protamines makes the DNA isolation procedure from sperm cells long and laborious. Cell lysis is also a challenging step because of the disulfide bonds-rich membranes of the sperm cells.
Hatice Sevim Nalkiran +4 more
doaj +1 more source
Comparative analysis of the DNA isolated from thyme leaves using different methods
Background. The base for a molecular analysis is DNA of high quality. For DNA isolation, different kits or classical methods are used. For mass analysis, isolation with kits is a very expensive process.
I. V. Bulavin +4 more
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Context: Dental tissue remains are the toughest, and chemically, the most stable tissue in the body. Its high resilience in the events of fire and bacterial decomposition makes them vital for DNA analysis by PCR method.
Prachi Suman +5 more
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Preservation and maceration of amazon açai leaflet tissue to obtain genomic DNA
The objective of this study was to test the efficiency of preservation and maceration methods for Euterpe precatoria leaflet tissue to obtain genomic DNA for molecular studies. The leaflets of E.
Hellen Sandra Freires da Silva Azêvedo +5 more
doaj +1 more source

