Results 41 to 50 of about 817,746 (315)

An efficient method for DNA extraction from Cladosporioid fungi

open access: yes, 2010
We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Bahkali, A.H.   +3 more
core   +1 more source

Simple and efficient protocol for isolation of high molecular weight DNA from Streptomyces aureofaciens

open access: yes, 1998
A simple and efficient protocol involving the use of cetyltrimethylammonium bromide (CTAB) for rapid isolation of high molecular wt (≥ 50 kb) DNA from Streptomyces aureofaciens is described.
Rawal, S.K., Tripathi, G.
core   +1 more source

Comparison of methods for isolating fungal DNA

open access: yesPractical Laboratory Medicine, 2021
Objectives: The main aim of this work was to compare the methods of DNA isolation in the moulds of genus Mucorales with special regard to the amount and purity of the DNA acquired. The acquired DNA was then amplified by specific real-time PCR.
Ilaria Galliano   +7 more
doaj   +1 more source

Comparison of gender determination from dental pulp and dentin after exposure to various environmental conditions: A polymerase chain reaction-based SRY gene study

open access: yesContemporary Clinical Dentistry, 2019
Purpose: Sex determination becomes the first priority by a forensic investigator in the process of identification of bodies mutilated beyond recognition due to mass disasters.
Ruheena Khan   +2 more
doaj   +1 more source

The Role of Hematopoietic Cell Transplantation in Ataxia‐Telangiectasia

open access: yesPediatric Blood &Cancer, EarlyView.
ABSTRACT Background Ataxia‐telangiectasia (A‐T) is a DNA repair disorder characterized by neurodegeneration, immunodeficiency, and cancer predisposition. Hematopoietic cell transplantation (HCT) is an established therapy in related disorders such as Fanconi anemia (FA) and Nijmegen breakage syndrome (NBS), but its role in A‐T is unclear.
Laila Alkhouli   +3 more
wiley   +1 more source

Comparison of methods for isolating fungal DNA

open access: yesCzech Journal of Food Sciences, 2011
In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as most detection techniques require DNA amplification ...
Petra MOŤKOVÁ, Jarmila VYTŘASOVÁ
doaj   +1 more source

Organoids in pediatric cancer research

open access: yesFEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

DNA extraction from formalin-fixed tissue: new light from the Deep-Sea

open access: yes, 2010
DNA samples were extracted from ethanol and formalin-fixed decapod crustacean tissue using a new method based on Tetramethylsilane (TMS)-Chelex. It is shown that neither an indigestible matrix of cross-linked protein nor soluble PCR inhibitors impede PCR
Thatje, S.   +14 more
core   +1 more source

PCR reaction testing by using microsatellite DNA markers on Bali Myna samples (Leucopsar rothschildi Stressemann, 1912)

open access: yesJurnal Biologi Udayana
Genetic research requires a good quality and quantity of DNA template and appropriate conditions to obtain optimal PCR results. This study aimed to evaluate and compare PCR reaction conditions and compositions in Bali Myna (Leucopsar rothschildi ...
Utami Lestari Budiawati   +2 more
doaj   +1 more source

Comparison of DNA Isolation Results with Simple Methods and Kits in Samples of Psidium guajava Leaves

open access: yesBiotropic: The Journal of Tropical Biology, 2018
DNA isolation is one of a series of methods that must be carried out on the basic techniques of Molecular Biology Analysis. Especially PCR-based molecular marking techniques. Many ways are done in DNA isolation. This study discusses the comparison of the
Yuanita Rachmawati   +1 more
doaj   +1 more source

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