Results 31 to 40 of about 817,746 (315)
Context: Dental tissue remains are the toughest, and chemically, the most stable tissue in the body. Its high resilience in the events of fire and bacterial decomposition makes them vital for DNA analysis by PCR method.
Prachi Suman +5 more
doaj +1 more source
Zinc finger recombinases with adaptable DNA sequence specificity [PDF]
Site-specific recombinases have become essential tools in genetics and molecular biology for the precise excision or integration of DNA sequences. However, their utility is currently limited to circumstances where the sites recognized by the recombinase ...
Proudfoot, C. +19 more
core +1 more source
Intrafamilial genotyping of Helicobacter pylori from faecal DNA [PDF]
Helicobacter pylori infection, often acquired in early childhood, is a global cause of undernutrition, gastritis, peptic ulcer disease and gastric carcinoma. This study tested the feasibility of using H.
Malcolm, C. +22 more
core +1 more source
Comparative analysis of human DNA extraction methods and mitochondrial DNA HV1 and HV2 haplogroup determination [PDF]
The high integrity of extracted DNA is necessary for the determination of human haplogroups, based on the mitochondrial DNA (mtDNA) marker sequences. The study aims were to isolate the total DNA from selected human samples and establish a protocol that ...
Nikezić Aleksandra G. +8 more
doaj
High-quality DNA extraction for Desmodium gangeticum: A medicinal shrub of Shivalik Himalayas
Background: Desmodium gangeticum, a crucial medicinal shrub from the Himalayan Shivalik region, is an economically important species globally. It contains a vast range of secondary metabolites, majorly polyphenols, that interfere with high-quality ...
Shiwani Singh +2 more
doaj +1 more source
The Miraprep: A Protocol that Uses a Miniprep Kit and Provides Maxiprep Yields. [PDF]
Plasmid purification is a basic tool of molecular biologists. Although the development of plasmid isolation kits utilizing silica spin columns reduced the time and labor spent on plasmid purification, achieving large plasmid DNA yields still requires ...
Mira I Pronobis +2 more
doaj +1 more source
Discovery of cyanophage genomes which contain mitochondrial DNA polymerase [PDF]
DNA polymerase γ is a family A DNA polymerase responsible for the replication of mitochondrial DNA in eukaryotes. The origins of DNA polymerase γ have remained elusive because it is not present in any known bacterium, though it has been hypothesized that
Whitworth, Anna L. +19 more
core +1 more source
Accurate isolation of targeted extracellular vesicle (EV) is challenging due to the heterogeneity of EV subpopulations which are from different cell origins. Most EV subpopulations lack a single marker whose expression cleanly distinguish them from mixed
Chaoyong, Yang +6 more
core +1 more source
Mitochondrial DNA from hamster tumors induced by adenovirus type 12
1. Mitochondria isolated from hamster tumors induced by adenovirus type 12 possessed circular DNA fibers. 2. The mean value of the length of the highest frequency group of the circular DNA molecules was 4.92 ±0.38 μ. 3. Catenated dimer DNA molecules
Oda, Takuzo, Nishida, Shigeru
core +1 more source
Determining species diversity of microfungal communities in forest tree roots by pure-culture isolation and DNA sequencing [PDF]
Pure-culture isolation from roots was compared with transformation of total DNA from roots followed by sequence analysis of ITS 1/2 rDNA of representative clones as methods for determining the abundance and composition of microbiota in roots of Betula ...
Bateman, G. L. +5 more
core +1 more source

