Results 21 to 30 of about 789,026 (265)
Detection of PCR inhibition in food and feed with a synthetic plasmid
Czech Journal of Food Sciences, 2017 We present a successful use of the plasmid inhibition detection and DNA isolation protocol optimisation for four food/feed samples in qPCR analysis of the sequence coding for chloroplast tRNA-Leu: two meat meal samples and two samples made of cranberries
Tereza Sovová +3 more
doaj +1 more source
High-quality DNA extraction for Desmodium gangeticum: A medicinal shrub of Shivalik Himalayas
Journal of Medicinal Plants for Economic DevelopmentBackground: Desmodium gangeticum, a crucial medicinal shrub from the Himalayan Shivalik region, is an economically important species globally. It contains a vast range of secondary metabolites, majorly polyphenols, that interfere with high-quality ...
Shiwani Singh +2 more
doaj +1 more source
International Journal of Tropical Veterinary and Biomedical Research, 2018 Elephant Training Center (ETC) Way Kambas National Park (WKNP) was built to support human-elephant mitigation conflict. The small population of captive sumatran elephant in ETC WKNP need a comprehensive strategy in order to maintain the genetic variation
Elly L. Rustiati +9 more
doaj +1 more source
The Miraprep: A Protocol that Uses a Miniprep Kit and Provides Maxiprep Yields. [PDF]
PLoS ONE, 2016 Plasmid purification is a basic tool of molecular biologists. Although the development of plasmid isolation kits utilizing silica spin columns reduced the time and labor spent on plasmid purification, achieving large plasmid DNA yields still requires ...
Mira I Pronobis +2 more
doaj +1 more source
Comparison of methods for isolating fungal DNA
Practical Laboratory Medicine, 2021 Objectives: The main aim of this work was to compare the methods of DNA isolation in the moulds of genus Mucorales with special regard to the amount and purity of the DNA acquired. The acquired DNA was then amplified by specific real-time PCR.
Ilaria Galliano +7 more
doaj +1 more source
Comparative analysis of human DNA extraction methods and mitochondrial DNA HV1 and HV2 haplogroup determination [PDF]
Kragujevac Journal of Science, 2020 The high integrity of extracted DNA is necessary for the determination of human haplogroups, based on the mitochondrial DNA (mtDNA) marker sequences. The study aims were to isolate the total DNA from selected human samples and establish a protocol that ...
Nikezić Aleksandra G. +8 more
doaj
Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
Jurnal Biologi UdayanaGenetic research requires a good quality and quantity of DNA template and appropriate conditions to obtain optimal PCR results. This study aimed to evaluate and compare PCR reaction conditions and compositions in Bali Myna (Leucopsar rothschildi ...
Utami Lestari Budiawati +2 more
doaj +1 more source
Biotropic: The Journal of Tropical Biology, 2018 DNA isolation is one of a series of methods that must be carried out on the basic techniques of Molecular Biology Analysis. Especially PCR-based molecular marking techniques. Many ways are done in DNA isolation. This study discusses the comparison of the
Yuanita Rachmawati +1 more
doaj +1 more source