Results 11 to 20 of about 789,026 (265)

Development of a multiplex pcr method for direct detection of common mastitis pathogens in bovine milk samples [PDF]

open access: yesKafkas Universitesi Veteriner Fakültesi Dergisi, 2017
The aim of this study was to evaluate a simple and rapid DNA extraction method combined with a multiplex polymerase chain reaction (mPCR) for the identification of the major mastitis pathogens (Staphylococcus aureus, Streptococcus agalactiae, Escherichia
Recep KALIN   +4 more
doaj   +1 more source

DNA isolation in processed chicken meat products (nugget) using modified DNeasy Mericon Food kit (Qiagen)

open access: yesHo Chi Minh City Open University Journal of Science - Engineering and Technology, 2022
DNA isolation is one of the important steps in conducting PCR-based or real-time PCR-based molecular analysis. This study aims to test the quality of DNA isolation extracted from samples of processed food products from chicken meat (chicken nuggets). The
Alfi Sophian   +2 more
doaj   +1 more source

Analysis of Purity and Concentration Escherichia coli DNA by Boiling Method Isolation with Addition of Proteinase-K and RNase

open access: yesIndonesian Journal of Medical Laboratory Science and Technology, 2023
Escherichia coli is a leading cause of Urinary Tract Infections (UTIs) in Indonesia, with approximately 180,000 cases reported annually. The more cases of UTIs, the more PCR diagnosis is needed with an accurate, fast, simple, and economical DNA ...
Bunga Rossa Lesiani   +3 more
doaj   +1 more source

Detection of toxigenic Clostridium difficile in pig feces by PCR

open access: yesVeterinární Medicína, 2009
: Clostridium difficile is considered an important uncontrolled cause of neonatal diarrhea. Also, the presence of bacteria in the feces of the animal could represent a zoonosic risk for the contamination of meat products.
S. Alvarez-Perez   +3 more
doaj   +1 more source

Magnetofection and isolation of DNA using polyethyleneimine functionalized magnetic iron oxide nanoparticles [PDF]

open access: yesRoyal Society Open Science, 2018
This study was carried out to develop a simple and efficient method to isolate DNA directly from biological samples using iron oxide nanoparticles (IONPs) functionalized with polyethyleneimine (PEI).
Adheesha N. Danthanarayana   +4 more
doaj   +1 more source

Extraction of high-quality genomic DNA and identification of different DNA barcoding markers for chickpea (Cicer arietinum L.)

open access: yesBuletin Agroteknologi, 2020
The genetic studies of individual plants, especially self-pollinated species like chickpea need to be evaluated at the DNA level with the help of molecular markers for identifying genetic variations among the plants.
Umavathi Saraswathi   +1 more
doaj   +1 more source

Comparison of DNA Isolation Methods that Derived from Leaves of a Potential Anti-Cancer Rodent Tuber (Typhonium flagelliforme) Plant

open access: yesInternational Journal of Agriculture System, 2022
The content of polysaccharides, polyphenols, proteins, and RNA compounds is the main problem often found in Plants DNA isolation, which inhibit the process of DNA isolation.
Gemilang Rahmadara   +5 more
doaj   +1 more source

DNA methylation dynamics associated with long-term isolation of simulated space travel

open access: yesiScience, 2022
Long-term isolation is one of the risk factors that astronauts will encounter in spaceflight. At present, few researches have explored DNA methylation dynamics during long-term isolation.
Fei Hou   +8 more
doaj   +1 more source

Comparison of gender determination from dental pulp and dentin after exposure to various environmental conditions: A polymerase chain reaction-based SRY gene study

open access: yesContemporary Clinical Dentistry, 2019
Purpose: Sex determination becomes the first priority by a forensic investigator in the process of identification of bodies mutilated beyond recognition due to mass disasters.
Ruheena Khan   +2 more
doaj   +1 more source

Comparison of methods for isolating fungal DNA

open access: yesCzech Journal of Food Sciences, 2011
In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as most detection techniques require DNA amplification ...
Petra MOŤKOVÁ, Jarmila VYTŘASOVÁ
doaj   +1 more source

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