Results 41 to 50 of about 1,916,984 (295)

Combined bacterial and fungal targeted amplicon sequencing of respiratory samples: Does the DNA extraction method matter?

PLoS ONE, 2020
BACKGROUND:High-throughput sequencing techniques are used to analyse the diversity of the respiratory microbiota in health and disease. Although extensive data are available regarding bacterial respiratory microbiota, its fungal component remains poorly ...
Cécile Angebault, Mathilde Payen, Paul-Louis Woerther, Christophe Rodriguez, Françoise Botterel   +4 more
doaj   +1 more source

A quantitative PCR approach to determine gene copy number [PDF]

, 2008
Here, we report on the use of quantitative PCR (qPCR) to determine gene copy number in filamentous fungi. Using the sequenced dothideomycete Stagonospora nodorum, qPCR was used to unequivocally confirm the presence of single, two and three copy regions ...
Hane, J., Oliver, Richard, Peter S. Solomon, Solomon, Peter S., Ipcho, S., Hane, J.K., Solomon, P., Ipcho, Simon V.S., Tan, K.C., Ipcho, S.V.S., Tan, Kar-Chun, James K. Hane, Oliver, R.P., Richard P. Oliver, Hane, James K., Kar-Chun Tan, Oliver, Richard P., Solomon, P.S., Simon V.S. Ipcho   +18 more
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Succession of wood-inhabiting fungal communities [PDF]

, 2013
Dead wood constitutes an important substrate for biodiversity in boreal forests. As the wood decays, fungal communities develop and species associations are formed.
Ottosson, Elisabet
core   +1 more source

Fungal CSL transcription factors [PDF]

, 2007
Background: The CSL (CBF1/RBP-J kappa/Suppressor of Hairless/LAG-1) transcription factor family members are well-known components of the transmembrane receptor Notch signaling pathway, which plays a critical role in metazoan development. They function as
Prevorovsky, M, Folk Petr, Folk, P, Puta, F, Půta František, Převorovský Martin   +5 more
core   +1 more source

General Fungal DNA Extraction v2

, 2023
This is a routine protocol for extracting DNA from various fungi. This extraction method is suitable for follow-up molecular work such as PCR amplification.
Angie Macias, Matthew T Kasson, Brian Lovett   +2 more
openaire   +1 more source

Core histones govern echinocandin susceptibility in Candida glabrata

Microbiology Spectrum
The dynamic chromatin structure regulates many biological processes including gene expression, DNA repair, and genome stability in eukaryotic cells.
Aditi Pareek, Rupinder Kaur
doaj   +1 more source

ITS2 REGION-BASED MOLECULAR IDENTIFICATION OF FUNGAL PATHOGENS IN EQUINE CORNEAL ULCERATION [PDF]

Assiut Veterinary Medical Journal, 2014
This study aimed to assess the utility of polymerase chain reaction (PCR) and DNA sequencing in diagnosing fungal ulcerative keratitis in horses and compare their sensitivity with conventional microbiologic techniques used in laboratories.
M. HAMED, HAROON MOHAMMAD, WENDY TOWNSEND, MOHAMED N. SELEEM   +3 more
doaj   +1 more source

Using DNA Barcoding in Fungal Taxonomy

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 2020
Totally, 1.5 million fungal species estimated, of which less than 10 percent have been described to date. Recent advances in molecular biology as well as gene sequencing technologies facilitate the discovery and identification of new species in various groups of living organisms, especially fungi.
Younes Rezaee Danesh, Semra Demir
openaire   +3 more sources

An overview of using fungal DNA for the diagnosis of invasive mycoses

Expert Review of Molecular Diagnostics, 2022
Fungal PCR has undergone considerable standardization and, together with the availability of commercial assays, external quality assessment schemes, and extensive performance validation data, is ready for widespread use for the screening and diagnosis of invasive fungal disease (IFD).Drawing on the experience and knowledge of the leads of the various ...
White, P Lewis, Alanio, Alexandre, Brown, Lottie, Cruciani, Mario, Hagen, Ferry, Gorton, Rebecca, Lackner, Michaela, Millon, Laurence, Morton, C Oliver, Rautemaa-Richardson, Riina, Barnes, Rosemary, Donnelly, J Peter, Loffler, Juergen   +12 more
openaire   +6 more sources

Database establishment for the secondary fungal DNA barcode translational elongation factor 1 alpha (TEF1 alpha)

, 2019
With new or emerging fungal infections, human/animal fungal pathogens are a growing threat worldwide. Current diagnostic tools are slow, non-specific at the species and subspecies levels, and require specific morphological expertise to accurately ...
Garcia-Hermoso, Dea, Muniz, Mauro de Medeiros, Cruz, Fundacao Oswaldo, Taylor, Maria Lucia, Chindamporn, Ariya, Irinyi, Laszlo, Velegraki, Aristea, Cano-Lira, Jose F., Dromer, Francoise, Sorrell, Tania C., Nenoff, Pietro, Desnos-Ollivier, Marie, Ranque, Stephane, Melo, Analy Salles de Azevedo, Moretti, Maria Luiza, Guarro, Josep, Arabatzis, Michael, Stubbe, Dirk, de Beer, Wilhelm, Meyer, Wieland, Arthur, Ian, Pais, Celia, Woo, Patrick C. Y., Hebert, Paul D. N., Halliday, Catriona, Minh Thuy Vi Hoang, Cardinali, Gianluigi, Lee, Chun-Yi, Nishikaku, Angela Satie, Chen, Sharon, Zancope-Oliveira, Rosely Maria, Castanon, Laura Rosio, Colombo, Arnaldo L., Hendrickx, Marijke, Tsang, Chi-Ching, de Almeida Soares, Celia Maria, Pchelin, Ivan Mikhailovich, Robbertse, Barbara, Uhrlass, Silke, Gryzenhout, Marieka, Robert, Vincent, Normand, Anne-Cecile, Piarroux, Renaud, Yurayart, Chompoonek, Toriello, Conchita, de Hoog, Sybren, Schoch, Conrad   +46 more
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