Results 41 to 50 of about 211,518 (253)

DNA- and RNA- Derived Fungal Communities in Subsurface Aquifers Only Partly Overlap but React Similarly to Environmental Factors

Microorganisms, 2019
Recent advances in high-throughput sequencing (HTS) technologies have revolutionized our understanding of microbial diversity and composition in relation to their environment.
Ali Nawaz, Witoon Purahong, Martina Herrmann, Kirsten Küsel, François Buscot, Tesfaye Wubet   +5 more
doaj   +1 more source

The Effectiveness of PCR in Diagnosis of Fungal Keratitis

Oftalʹmologiâ, 2020
Fungi implicated in mycotic keratitis include different species. Conventional methods for the diagnosis of fungal keratitis include staining of corneal scarpings, culture medium (Sabouraud agar) for isolating fungi.Purpose.
G. I. Krichevskaya, L. A. Kovaleva, I. D. Zyurnyayeva, P. V. Makarov, A. E. Andryushin   +4 more
doaj   +1 more source

Engineering Antigen Density on M13 Bacteriophage for Personalized Nanovaccine

Advanced Healthcare Materials, EarlyView.
Increasing antigen density on the phage capsid impacts phage uptake and antigen cross‐presentation by antigen‐presenting cells, leading to enhanced activation of antitumor CD8+ T cells in the draining lymph nodes. These activated CD8+ T cells subsequently recognize the antigen‐expressing tumors and mediate their specific cytotoxic killing, thereby ...
Shengnan Huang, Allison Madow, Jack Rudrum, Huaiyao Peng, Hao Nghi Luu, Peiyan Dong, Jifa Qi, Angela M. Belcher   +7 more
wiley   +1 more source

Protocol for determination of phosphatidylinositol 3-phosphate levels and localization in Candida glabrata by confocal microscopy

STAR Protocols
Summary: Phosphatidylinositol 3-phosphate (PI3P) levels govern membrane trafficking in Candida glabrata. Here, we present a confocal imaging-based protocol for PI3P localization analysis using the GFP-FYVE (found in Fab1, YOTB, Vac1, and EEA1) fusion ...
Fizza Askari, Rupinder Kaur
doaj   +1 more source

RNAi in the Rhizarian Phytopathogen Plasmodiophora brassicae: The Causal Agent of Clubroot Disease in Cruciferous Crops

Advanced Science, EarlyView.
This study uncovers an unusual RNAi pathway in the rhizarian pathogen Plasmodiophora brassicae. In the absence of Dicer, a Drosha‐like RNase III protein supports the biogenesis of predominant 21‐nt small RNAs, and two Argonaute proteins mediate small RNA‐guided silencing.
Xiong Zhang, Yupo Wu, Jiachang Hou, Lixia Gao, Longqi Pan, Cuicui Shen, Chuanji Zhao, Feng Gao, Yi Zhang, Li Ren, Xiaoping Fang, Xiaoling Dun, Shengyi Liu, Lijiang Liu   +13 more
wiley   +1 more source

Aspartyl proteases target host actin nucleator complex protein to limit epithelial innate immunity

EMBO Reports
Epithelial-immune cell communication is pivotal to control microbial infections. We show that glycosylphosphatidylinositol-linked aspartyl proteases (Yapsins) of the human opportunistic pathogenic yeast Candida glabrata (Cg) thwart epithelial cell (EC ...
Sandip Patra, Rupinder Kaur
doaj   +1 more source

Reusable Biosensor for Easy RNA Detection from Unfiltered Saliva

Sensors
Biosensors are transforming point-of-care diagnostics by simplifying the detection process and enabling rapid, accurate testing. This study introduces a novel, reusable biosensor designed for direct viral RNA detection from unfiltered saliva, targeting ...
Paweł Wityk, Agata Terebieniec, Robert Nowak, Jacek Łubiński, Martyna Mroczyńska-Szeląg, Tomasz Wityk, Dorota Kostrzewa-Nowak   +6 more
doaj   +1 more source

Uncovering unseen fungal diversity from plant DNA banks [PDF]

PeerJ, 2017
Throughout the world DNA banks are used as storage repositories for genetic diversity of organisms ranging from plants to insects to mammals. Designed to preserve the genetic information for organisms of interest, these banks also indirectly preserve ...
Erin M. Datlof, Anthony S. Amend, Kamala Earl, Jeremy Hayward, Clifford W. Morden, Rachael Wade, Geoffrey Zahn, Nicole A. Hynson   +7 more
doaj   +2 more sources

Engineering Microbial Particles for Next‐Generation Biomedical Platforms

Advanced Science, EarlyView.
Microbe‐derived particles (MDPs), which include extracellular vesicles, outer membrane vesicles, inclusion bodies, polysaccharide particles, and virus‐like particles, represent a rapidly expanding category of bioinspired nanomaterials. With their natural origin, intrinsic biocompatibility, and highly programmable functionality, MDPs serve as a ...
Yuting Li, Yunjie Lu, Ziyan Huang, Jianjiang Chen, Yanbin Liu, Lei Qin, Chao Wang, Yumin Wu   +7 more
wiley   +1 more source

Morphological and molecular identification of an unknown fungal isolate from Al‐Dujail District: A new record of Tulostoma winterhoffii in Iraq

Indonesian Journal of Biotechnology
The Tulostoma genus, known as stalkballs or stalked puffballs, belongs to the Agaricaceae family. This study was designed to identify an unknown fungal species collected from the Al‐Dujail district in Iraq based on morphological examination and molecular
Taghreed Khudhur Mohammed, Hanaa Naji Abdullah, Sinai Waleed Mohammed, Muntadher Fadhil Jassim   +3 more
doaj   +1 more source