Results 41 to 50 of about 201,219 (249)

The use of flow cytometry for fungal nuclear DNA quantification [PDF]

open access: yesCytometry Part A, 2021
AbstractGenome size information is sparse across fungi, with information being available for less than 2000 species. So far, most records have been obtained using static, microscope‐based cytometry methods or derived from genome sequencing projects. Flow cytometry is now considered the state‐of‐the‐art method for obtaining genome size measurements, and
Pedro Talhinhas   +2 more
openaire   +3 more sources

ITS2 REGION-BASED MOLECULAR IDENTIFICATION OF FUNGAL PATHOGENS IN EQUINE CORNEAL ULCERATION [PDF]

open access: yesAssiut Veterinary Medical Journal, 2014
This study aimed to assess the utility of polymerase chain reaction (PCR) and DNA sequencing in diagnosing fungal ulcerative keratitis in horses and compare their sensitivity with conventional microbiologic techniques used in laboratories.
M. HAMED   +3 more
doaj   +1 more source

Using DNA Barcoding in Fungal Taxonomy

open access: yesYüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 2020
Totally, 1.5 million fungal species estimated, of which less than 10 percent have been described to date. Recent advances in molecular biology as well as gene sequencing technologies facilitate the discovery and identification of new species in various groups of living organisms, especially fungi.
Younes Rezaee Danesh, Semra Demir
openaire   +3 more sources

Fungal CTAB DNA Extraction v1 [PDF]

open access: yes, 2020
This protocol is a CTAB DNA extraction method for filamentous fungi. Its purpose is to extract high molecular weight genomic DNA for genome sequencing.
Derreck Carter-House   +3 more
openaire   +1 more source

An overview of using fungal DNA for the diagnosis of invasive mycoses

open access: yesExpert Review of Molecular Diagnostics, 2022
Fungal PCR has undergone considerable standardization and, together with the availability of commercial assays, external quality assessment schemes, and extensive performance validation data, is ready for widespread use for the screening and diagnosis of invasive fungal disease (IFD).Drawing on the experience and knowledge of the leads of the various ...
White, P Lewis   +12 more
openaire   +6 more sources

Core histones govern echinocandin susceptibility in Candida glabrata

open access: yesMicrobiology Spectrum
The dynamic chromatin structure regulates many biological processes including gene expression, DNA repair, and genome stability in eukaryotic cells.
Aditi Pareek, Rupinder Kaur
doaj   +1 more source

General Fungal DNA Extraction v2

open access: yes, 2023
This is a routine protocol for extracting DNA from various fungi. This extraction method is suitable for follow-up molecular work such as PCR amplification.
Angie Macias   +2 more
openaire   +1 more source

CAR T‐Cell Therapy in Neurology: A Scoping Review of Neuro‐Oncology, Autoimmune Diseases & Neurotoxicity

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Chimeric antigen receptor (CAR) T‐cell therapy has been investigated in neurological diseases, encompassing both central nervous system malignancies and autoimmune disorders, thereby extending its application beyond hematological cancers.
Omar Alqaisi   +5 more
wiley   +1 more source

The Effectiveness of PCR in Diagnosis of Fungal Keratitis

open access: yesOftalʹmologiâ, 2020
Fungi implicated in mycotic keratitis include different species. Conventional methods for the diagnosis of fungal keratitis include staining of corneal scarpings, culture medium (Sabouraud agar) for isolating fungi.Purpose.
G. I. Krichevskaya   +4 more
doaj   +1 more source

Spatial structure of fungal DNA assemblages revealed with eDNA metabarcoding in a forest river network in western Japan [PDF]

open access: yesMetabarcoding and Metagenomics, 2019
Growing evidence has revealed high diversity and spatial heterogeneity of fungal communities in local habitats of terrestrial ecosystems. Recently, the analysis of environmental DNA has been undertaken to study the biodiversity of organisms, such as ...
Shunsuke Matsuoka   +5 more
doaj   +3 more sources

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