Results 51 to 60 of about 211,518 (253)

ITS2 REGION-BASED MOLECULAR IDENTIFICATION OF FUNGAL PATHOGENS IN EQUINE CORNEAL ULCERATION [PDF]

open access: yesAssiut Veterinary Medical Journal, 2014
This study aimed to assess the utility of polymerase chain reaction (PCR) and DNA sequencing in diagnosing fungal ulcerative keratitis in horses and compare their sensitivity with conventional microbiologic techniques used in laboratories.
M. HAMED   +3 more
doaj   +1 more source

The Plant‐to‐Plant Circular Strategy: Coupling Photodegradation and Phytoremediation With Plant‐Based Nanomaterials for Plastic Degradation

open access: yesAdvanced Science, EarlyView.
Plant‐based nanomaterials derived from alfalfa serve as dual‐function catalysts, coupling photodegradation and phytoremediation to degrade polyethylene under UV‐A light and in soil. The nanomaterials work synergistically with alfalfa and soil microbes, achieving efficient plastic breakdown without trophic transfer.
Haoran Liu   +4 more
wiley   +1 more source

Modified Phenol-Chloroform Method for Dermatophyte DNA Extraction: Optimization of Culture Media and Extraction Steps to Improve Quality and Quantity of Fungal DNA

open access: yesJournal of Pure and Applied Microbiology
Complex procedures are required for DNA extraction from filamentous fungi owing to the structural complexity of their cell walls. Disruption of fungal cells results in the release of large amounts of polysaccharides and proteins.
A. Jain Pavithra   +4 more
doaj   +1 more source

Drought Stress Mediated Changes in Food Crops: Mechanisms and Remediation Strategies

open access: yesAdvanced Science, EarlyView.
This work presents a multi‐scale framework for flavonoid‐mediated drought tolerance in staple food crops, integrating ROS redox regulation, antioxidant defense, rhizosphere microbial interactions, and sustainable agronomic practices. This interdisciplinary model provides core insights to develop climate‐resilient cropping systems and enhance global ...
Xiaoyi Duan   +5 more
wiley   +1 more source

The Impact of DNA Extraction Methods on Stool Bacterial and Fungal Microbiota Community Recovery

open access: yesFrontiers in Microbiology, 2019
Our understanding of human gut microbiota in health and disease depends on accurate and reproducible microbial data acquisition. The critical step in this process is to apply an appropriate methodology to extract microbial DNA, since biases introduced ...
Kristýna Fiedorová   +12 more
doaj   +1 more source

Functional–Taxonomic Scaling Resolves Conflicting Average Genome Size Estimates Across Environmental Gradients

open access: yesAdvanced Science, EarlyView.
Based on genome size‐functional breadth theory, we introduce and validate a framework that adjudicates conflicting average genome size (AGS) estimates by testing which estimate yields a gap between scaled functional diversity and scaled taxonomic diversity that is consistent with the expected positive association between genome size and gene functional
Huanhuan Zhu   +8 more
wiley   +1 more source

Sex‐ and Time‐Resolved Physiological and Gut Ecosystem Responses to a Food‐Grade Microbiome‐Targeted Intervention in Collegiate Endurance Athletes

open access: yesAdvanced Science, EarlyView.
In collegiate endurance athletes, a 6‐week randomized CPYE intervention yielded time‐ and sex‐dependent shifts in field‐based cardiorespiratory, metabolic, and redox indices, accompanied by changes in gut multi‐omics profiles. An exploratory extension during intensified training generates hypotheses warranting future validation.
Meng Zhang   +8 more
wiley   +1 more source

Evaluation of lake sedimentary ancient DNA metabarcoding to assess fungal biodiversity in Arctic paleoecosystems

open access: yesEnvironmental DNA, 2022
Fungi are crucial organisms in most ecosystems as they exert ecological key functions and are closely associated with land plants. Fungal community changes may, therefore, help reveal biodiversity changes in past ecosystems.
Peter Andreas Seeber   +6 more
doaj   +1 more source

Synthetic Shewanella‐Bacillus Microbial Consortia for Enhanced Metal Oxide Leaching From Spent Lithium‐Ion Batteries Using Brewing Wastewater as Leaching Agent

open access: yesAdvanced Science, EarlyView.
A recycling approach of spent lithium‐ion batteries is developed using brewing wastewater as the leaching agent and an engineering microbial consortium as the biocatalyst. Shewanella oneidensis reduces spent LiNixCoyMn1‐x‐yO2 cathodes via extracellular electron transfer, while Bacillus subtilis removes residual ethanol to eliminate inhibition.
Baocai Zhang   +14 more
wiley   +1 more source

Stage-specific DNA methylation in a fungal plant pathogen [PDF]

open access: yesJournal of Bacteriology, 1986
Significantly more 5-methylcytosine residues were found in the DNA from the dormant sclerotia of Phymatotrichum omnivorum than in the DNA from the metabolically active mycelia of the fungus, as shown by high-pressure liquid chromatography of acid-hydrolyzed DNA digests and by restriction of the DNA with the isoschizomers MspI and HpaII.
E R, Jupe, J M, Magill, C W, Magill
openaire   +2 more sources

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