Results 71 to 80 of about 201,219 (249)

The Influence of DNA Extraction and Lipid Removal on Human Milk Bacterial Profiles

Methods and Protocols, 2020
Culture-independent molecular techniques have advanced the characterization of environmental and human samples including the human milk (HM) bacteriome. However, extraction of high-quality genomic DNA that is representative of the bacterial population in
Anna Ojo-Okunola, Shantelle Claassen-Weitz, Kilaza S. Mwaikono, Sugnet Gardner-Lubbe, Heather J. Zar, Mark P. Nicol, Elloise du Toit   +6 more
doaj   +1 more source

Macrophage Extracellular Traps in Immunity and Cancer

Advanced Science, EarlyView.
As a macrophage‐mediated innate defense mechanism, the dysregulated release of METs drives chronic inflammation and influences tumor progression. Furthermore, METs exhibit a functional duality within the tumor microenvironment, capable of both promoting and suppressing tumor development.
Junyao Li, Eugenie Nepovimova, Adam Grundel, Lukas Peter, Qinghua Wu, Kamil Kuca   +5 more
wiley   +1 more source

Optimal liquid-based DNA preservation for DNA barcoding of field-collected fungal specimens

Heliyon
Preserving fungal tissue DNA in the field is essential for molecular ecological research, enabling the study of fungal biodiversity and community dynamics. This study systematically compares two liquid-based preservation solutions, RNAlater and DESS, for
Yu-Ja Lee, Guan Jie Phang, Che-Chih Chen, Jie-Hao Ou, Yu-Hsuan Fan, Yin-Tse Huang   +5 more
doaj   +1 more source

Consumed by Abdominal Distention

Arthritis Care &Research, EarlyView.
Abimbola Fadairo‐Azinge, James Grenert, Brian Haas, Mary Margaretten   +3 more
wiley   +1 more source

Multimodal Imaging Reveals Rapid Catecholamine Uptake and Release by Neutrophils

Advanced Science, EarlyView.
We show that immune cells (neutrophils) synthesize, uptake, and store catecholamine neurotransmitters such as dopamine or adrenaline. They also release them in response to specific stimuli (serotonin), which we directly visualize using fluorescent nanosensors. We further demonstrate that catecholamines affect neutrophil functions (NETosis) and platelet
Jennifer Mohr, Anne Schmitz, Meshkat Dinarvand, Franziska Wulfert, Sangeetha Shankar, Bjoern F. Hill, Michael Wojak, Juliana Gretz, Marie Britz, Elsa Neubert, Magdalena Shumanska, Sofia Kaushik, Linda Kartaschew, Ivan Bogeski, James Daniel, Sebastian Jung, Johannes Eble, Guido Wabnitz, Luise Erpenbeck, Sebastian Kruss   +19 more
wiley   +1 more source

Primer pairs, PCR conditions, and peptide nucleic acid clamps affect fungal diversity assessment from plant root tissues

Mycology
High-throughput sequencing has become a prominent tool to assess plant-associated microbial diversity. Still, some technical challenges remain in characterising these communities, notably due to plant and fungal DNA co-amplification.
Chloé Viotti, Michel Chalot, Peter G. Kennedy, François Maillard, Sylvain Santoni, Damien Blaudez, Coralie Bertheau   +6 more
doaj   +1 more source

Next Generation Sequencing of Ancient Fungal Specimens: The Case of the Saccardo Mycological Herbarium

Frontiers in Ecology and Evolution, 2018
Despite their essential role in the environment, the number of known fungal species is still low compared to the recent estimates of the fungal biodiversity (from 2.2 to 3.8 million species), principally because of their often cryptic or ambiguous ...
Niccolò Forin, Niccolò Forin, Sebastiano Nigris, Samuele Voyron, Mariangela Girlanda, Alfredo Vizzini, Giorgio Casadoro, Barbara Baldan, Barbara Baldan   +8 more
doaj   +1 more source

Rapid extraction of fungal DNA for PCR amplification

Nucleic Acids Research, 1992
Peer ...
openaire   +5 more sources

Crystal Facet‐Polysaccharide Matching in CFS‐P Nanocrystals Drives Fungal Uptake, Vacuole Destruction, and Selective Antifungal Activity

Advanced Science, EarlyView.
Facet‐engineered PVP‐capped CuFeSe2 nanocrystals exhibit potent, selective antifungal activity by strongly binding cell‐wall mannan, enabling vacuolar entry and organelle disruption. High‐index facets show superior mannan affinity, validated by theoretical calculations and experiments. CFS‐P induces vacuolar cavitation, mitochondrial abnormalities, and
Zhaohui Wang, Xiaojin Deng, Xujuan Huang, Yuhang Pan, Yao Qin, Lu Zhang, Le Xu, Tianyi Qin, Deteng Zhang, Yi Ma, Yalong Wang   +10 more
wiley   +1 more source

Spontaneous Non‐Catalyzed Molecular Reactions and Interactions in the Human Body: Biomedical Implications

Advanced Science, EarlyView.
ABSTRACT The human body functions as a natural reactor for a vast network of chemical and biological reactions and physical interactions among small molecules, proteins, cells, and numerous other components. These reactions/interactions are essential for maintaining normal physiological functions.
Yuhao Cai, Chao Zhao
wiley   +1 more source