Sigma1R restores mitochondrial energy metabolism via the IRE1α/XBP1 pathway. [PDF]
Sci RepYan W +5 more
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Fura-2 fluorescence is localized to mitochondria in endothelial cells
American Journal of Physiology-Cell Physiology, 1987 The new, highly fluorescent, calcium-sensitive dye, fura-2, can be loaded nondisruptively into intact cells by means of its permeant ester and used to measure the free calcium ion concentration in individual cells. For fura-2 to signal cytosolic calcium, it must be distributed homogeneously and exclusively throughout the cytoplasmic space.
S F, Steinberg +2 more
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Photobleaching of fura-2 and its effect on determination of calcium concentrations
American Journal of Physiology-Cell Physiology, 1987 This study was performed to determine the effect of photobleaching on the spectral properties of the calcium-sensitive fluorescent dye fura-2. Fura-2, whether in cells or in calibrating solutions, was found to be bleached when exposed to excitation light. In contrast to the widely held belief, photobleaching altered the spectral properties of the dye.
P L, Becker, F S, Fay
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Inhibition of Fura-2 sequestration and secretion with organic anion transport blockers
Cell Calcium, 1990 Fura-2 is widely used to measure the concentration of cytosolic free calcium, but in many cells the dye does not remain localized within the cytoplasmic matrix. In these cells, Fura-2 is sequestered within intracellular organelles, secreted into the extracellular medium, or both.
DI VIRGILIO, Francesco +2 more
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Continuous monitoring of Ca2+ uptake in membrane vesicles with fura-2
American Journal of Physiology-Cell Physiology, 1988 The Ca2+-selective, fluorescent dye, fura-2, was used to monitor ATP-dependent Ca2+ uptake by membrane vesicles isolated from rabbit skeletal muscle. Micromolar fura-2 concentrations, added outside the vesicles, served as a high-affinity, low-capacity Ca2+ buffer.
M E, Kargacin +2 more
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Advances in the use of the fluorescent probe fura-2 for the estimation of intrasynatposomal calcium
Journal of Neuroscience Research, 1992AbstractFura‐2 has been used to measure intracellular Ca2+ with great success in a variety of cell and subcellular preparations, including synaptosomes. There is however, a great deal of variability in the reported estimates of resting intrasynaptosomal Ca2+ ([Ca2+]i).
S L, Yates, E N, Fluhler, P M, Lippiello
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Fluorescent properties of rat cardiac trabeculae microinjected with fura-2 salt
American Journal of Physiology-Heart and Circulatory Physiology, 1993 We have measured force, sarcomere length, and Ca2+ during twitches in rat cardiac trabeculae. To avoid the difficulties associated with fura-2/acetoxymethyl ester (AM), fura-2 salt was iontophoretically microinjected into the preparation using a single impalement site; this is possible because fura-2 diffuses through the gap junctions between cells ...
P H, Backx, H E, Ter Keurs
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The estimation of free calcium within synaptosomes and mitochondria with fura-2; comparison to quin-2 [PDF]
Neurochemistry International, 1987 The utility of the acetoxymethyl esters of two tetracarboxylic acids, fura-2 and quin-2, in the determination of ionic calcium levels within synaptosomes and mitochondria was compared.
S C Bondy, H Komulainen
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Fura-2 diffusion and its use as an indicator of transient free calcium changes in single striated muscle cells [PDF]
FEBS Letters, 1986 Two questions bearing on the use of fura-2 to measure transient changes in intracellular Ca2+ concentration have been addressed. To investigate fura-2 intracellular binding, the amounts of fura-2 and [14C]glycine in Balanus nubilus myofibrillar bundles ...
C C Ashley
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