Results 161 to 170 of about 24,447,932 (192)
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Tetrahydroaminoacridine and related compounds interfere with fura-2 and indo-1
European Journal of Pharmacology: Molecular Pharmacology, 1992In earlier experiments, using a fluorimetric method (fura-2), we found what seemed to be a decreased cytoplasmic [Ca2+] in neuroblastoma cells when 1,2,3,4-tetrahydro-9-aminoacridine (THA) was added, but discovered by repeating our work under cell-free conditions, that THA affected the Ca(2+)-fura-2 signal.
L, Järlebark, E, Heilbronn
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Fura-2 fluorescent technique for the assessment of Ca2+ homeostasis in cardiomyocytes
Molecular and Cellular Biochemistry, 1997Ca2+ homeostasis plays a pivotal role in maintaining cell growth and function. Many heart diseases are related to the abnormalities in Ca2+ mobilization and extrusion. Ca2+-sensitive fluorescent dyes have been used successfully to estimate intracellular free Ca2+ ([Ca2+]i) level and the mechanisms of Ca2+ movements in living cells.
Y J, Xu, Q, Shao, N S, Dhalla
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Characterization of Voltage-Dependent Calcium Influx in Human Erythrocytes by fura-2
Biochemical and Biophysical Research Communications, 1997Thus far, the methods used to determine erythrocyte Ca2+ influx have not allowed the assessment of the kinetics of ion uptake. To overcome this drawback, we studied a new method, using the fluorescent Ca2+-chelator fura-2, which directly quantifies intracellular Ca2+ changes in human erythrocytes.
L. Soldati +7 more
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Journal of Neuroscience Methods, 1994
Intracellular calcium ions (Ca2+) play important roles in cell functions. Measurements of intracellular calcium ion concentration ([Ca2+]i) are often made with the fura-2 fluorescence recording technique in various preparations including neurons. Fura-2 has, however, a Ca(2+)-chelating action which complicates the interpretation of experimental results.
H, Tatsumi, Y, Katayama
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Intracellular calcium ions (Ca2+) play important roles in cell functions. Measurements of intracellular calcium ion concentration ([Ca2+]i) are often made with the fura-2 fluorescence recording technique in various preparations including neurons. Fura-2 has, however, a Ca(2+)-chelating action which complicates the interpretation of experimental results.
H, Tatsumi, Y, Katayama
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Analytical Biochemistry, 1992
Several authors have reported unexpected intracellular spectra of both indo-1 and fura-2. One of the major methodological problems in the evaluation of calcium concentration using fluorescent probes is that it is assumed that only two forms of the dyes are detectable within the cells.
F, Bancel +4 more
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Several authors have reported unexpected intracellular spectra of both indo-1 and fura-2. One of the major methodological problems in the evaluation of calcium concentration using fluorescent probes is that it is assumed that only two forms of the dyes are detectable within the cells.
F, Bancel +4 more
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Reassessment of fura-2 and the ratio method for determination of intracellular Ca2+ concentrations
Cell Calcium, 1991To determine intracellular Ca2+ concentrations more accurately, we examined Kd of Fura-2 for Ca2+ in conditions which were systemically changed. In a solution comprising of 150 mM KCI, 20 mM MOPS-KOH, pH 6.94, 60-100 microM EGTA and 1 microM Fura-2, Kd at 20 degrees C was 0.266 +/- 0.016 microM (mean +/- SEM) (21 determinations). The ionic strength (I)
A, Uto, H, Arai, Y, Ogawa
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Fura-2 handling in a polarized epithelial barrier: The toad urinary bladder
Life Sciences, 1992Toad bladders sacs were placed inside quartz cuvettes. When fura-2 AM was added to the mucosal compartment, low temperature (4 degrees C) almost completely blocked the transepithelial transfer of fluorescence observed at 20 degrees C (20 degrees C = 371 +/- 56, 4 degrees C = 29 +/- 29 fluorescence intensity in arbitrary units (FIAU), excitation at 340 ...
M, Parisi +3 more
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Tyrosine kinase inhibitors and Ca2+ signaling: direct interactions with fura-2
European Journal of Pharmacology, 2000Selective inhibitors were used to study the role of tyrosine kinases in alpha(1A)-adrenoceptor-mediated responses in transfected PC12 cells. Ca(2+) responses to noradrenaline were measured using fura-2, and the effects of genistein, tyrphostin A25, and herbimycin A were examined.
A, Berts, K P, Minneman
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Intracellular calcium responses of circadian pacemaker neurons measured with fura-2
Brain Research, 1994The circadian pacemaker in the eye of the mollusk Bulla gouldiana is located within basal retinal neurons (BRNs) that express a circadian rhythm in cell culture. Light and other depolarizing stimuli shift the phase of the pacemaker in the eye through a process that requires extracellular calcium and is blocked by Ni2+.
M E, Geusz, S, Michel, G D, Block
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Naunyn-Schmiedeberg's Archives of Pharmacology, 2008
Due to its high sensitivity, reproducibility and reliability, fura-2 is one of the most preferred tools to quantify Ca2+ levels. Recently, using fura-2/AM, some calcium channel blockers (CCBs) have been reported to have inhibitory effects on intracellular Ca2+ signaling even in nonexcitable cells that do not possess voltage-dependent Ca2+ channels ...
Masayoshi, Asai +8 more
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Due to its high sensitivity, reproducibility and reliability, fura-2 is one of the most preferred tools to quantify Ca2+ levels. Recently, using fura-2/AM, some calcium channel blockers (CCBs) have been reported to have inhibitory effects on intracellular Ca2+ signaling even in nonexcitable cells that do not possess voltage-dependent Ca2+ channels ...
Masayoshi, Asai +8 more
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