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A study of the precision of confocal, ratiometric, Fura-2-based [Ca2+] measurements

Cell Calcium, 1997
The precision with which quantitative confocal ratiometric Fura-2-based calcium measurements can be performed has been investigated. A standard confocal scanning laser microscope system has been modified so as to enable excitation with the 351 nm and 364 nm lines of the UV argon laser and simultaneous separation of the fluorescence emanating from the ...
Helm, P., Patwardhan, A., Manders, E.
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Interactions between Benzylamiloride and Fura-2: Studiesin Vitroand in Cardiac Myocytes

Archives of Biochemistry and Biophysics, 1998
Amiloride derivatives are commonly used inhibitors of Na+/H+- and Na+/Ca2+-exchange. Because they are fluorescent molecules the use of benzylamiloride (BZA), an inhibitor of Na+/Ca2+ exchange, in conjunction with Fura-2, a commonly used fluorescent Ca2+ indicator, might complicate interpretation of fluorescence data obtained.
C A, Hudson +4 more
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The use of Fura-2 to estimate myoplasmic [Ca2+] in human skeletal muscle

Cell Calcium, 1989
Fura-2 was used to estimate myoplasmic [Ca2+] in intact fibers and fiber segments from normal and diseased human muscles. Small muscle bundles (20-50 fibers) were loaded with the membrane-permeant form of the dye (Fura-2 AM). High-performance liquid chromatography was utilized to study the ability of these cells to hydrolyze Fura-2 AM.
P A, Iaizzo +3 more
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Intracellular concentrations of Fura-2 and Fura-2/AM in vascular smooth muscle cells following perfusion loading of Fura-2/AM