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Gene-trait matching among <i>Bifidobacterium dentium</i> strains reveals various glycan metabolism loci including a strain-specific fucosyllactose utilization cluster. [PDF]

open access: yesFront Microbiol
Catalano Gonzaga O   +7 more
europepmc   +1 more source
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β-Galactosidase

Cold Spring Harbor Protocols, 2017
β-galactosidase is used as a reporter for the quantitative analysis of gene expression. It is also used as a histochemical marker. This introduction briefly reviews the enzymatic reactions catalyzed by β-galactosidase and methods for assaying β-galactosidase activity.
Pradeep D, Uchil   +2 more
openaire   +2 more sources

The discovery of β-galactosidase

Trends in Biochemical Sciences, 1989
The enzyme beta-galactosidase was first mentioned in the literature by Beijerinck exactly a hundred years ago. The Department of Microbiology and Enzymology of the Delft University of Technology keeps the memory of Beijerinck, its first professor, alive by maintaining a 'Beijerinck-room' in the attic of the building.
Rouwenhorst, R.J. (author)   +2 more
openaire   +4 more sources

The formation of β-galactosidase in soil [PDF]

open access: possibleFolia Microbiologica, 1972
The activity of β-galactosidase during incubation of soil with lactose was studied. Enzyme activity reached its maximum between 6–24 h incubation. The addition of secondary ammonium phosphate stimulated the enzyme formation. Casein hydrolysate alone did not influence significantly enzyme formation even after a 72 h incubation.
P. Ryšavý, J. Macura
openaire   +2 more sources

Plasma α-galactosidase A: Properties and comparisons with tissue α-galactosidases

Biochimica et Biophysica Acta (BBA) - Enzymology, 1978
The human plasma form of alpha-galactosidase A (alpha-D-galactoside galactohydrolase, EC 3.2.1.22) was highly purified and exhibited apparent Km values of 1.9 mM with 4-methylumbelliferyl-alpha-D-galactopyranoside and 0.23 mM with globotriglycosylceramide.
David E. Bishop, Charles C. Sweeley
openaire   +3 more sources

The assay of β-galactosidase in soil

Folia Microbiologica, 1972
The method for the assay of β-galactosidase introduced by Lederberg (1950) was modified and used for studies of the enzyme in the soil.o-Nitrophenyl-β-d-galactoside served as substrate. Trismaleinate buffer was found to be more suitable than phosphate buffer in the assay.
P. Ryšavý, J. Macura
openaire   +3 more sources

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