Results 251 to 260 of about 401,087 (313)
Some of the next articles are maybe not open access.
Journal of Chromatography B: Biomedical Sciences and Applications, 1981
It is assumed that heparin is a heterogeneous substance. In order to further investigate the purification of heparin, a column chromatographic technique for the fractionation of heparin is described using various diameters of bead form cross-linked dextran gels and an automated apparatus. It was observed that Sephadex G-50 resulted in the separation of
R, Losito, H, Gattiker, G, Bilodeau
openaire +2 more sources
It is assumed that heparin is a heterogeneous substance. In order to further investigate the purification of heparin, a column chromatographic technique for the fractionation of heparin is described using various diameters of bead form cross-linked dextran gels and an automated apparatus. It was observed that Sephadex G-50 resulted in the separation of
R, Losito, H, Gattiker, G, Bilodeau
openaire +2 more sources
Gel diffusion chromatography of dextran
Journal of Chromatography A, 1962Abstract Glucose and dextrans of molecular weight 5,000 to 300,000 can be partially separated chromatographically on columns of agar (6–9 %) with buffer (pH 8.0) or water as eluant. Agarose gels (4 %) can also be used with water as eluant. The method can be used to estimate approximately the molecular weight distribution in small samples of clinical ...
B C, HUMMEL, D C, SMITH
openaire +2 more sources
Current Protocols in Molecular Biology, 1998
AbstractGel filtration (GF) chromatography separates proteins solely on the basis of molecular size. Separation is achieved using a porous matrix to which the molecules, for steric reasons, have different degrees of access‐‐i.e., smaller molecules have greater access and larger molecules are excluded from the matrix. Hence, proteins are eluted from the
openaire +3 more sources
AbstractGel filtration (GF) chromatography separates proteins solely on the basis of molecular size. Separation is achieved using a porous matrix to which the molecules, for steric reasons, have different degrees of access‐‐i.e., smaller molecules have greater access and larger molecules are excluded from the matrix. Hence, proteins are eluted from the
openaire +3 more sources
Gel Permeation Chromatography of Heparins
Thrombosis and Haemostasis, 1980SummaryGel permeation chromatography of heparins using water as solvent has recently been used to obtain fractions for biological investigation. We find that heparin fractions obtained in this way are not, necessarily, differentiated simply on a molecular size basis as has been alleged.
B, Mulloy, E A, Johnson
openaire +2 more sources
Gel matrices for scanning gel chromatography
Biophysical Chemistry, 1979I have examined the light-scattering behavior of a number of gel matrices used in gel filtration chromatography. The angular dependence of light scattering by Sephadexes is consistent with treatment of the particles as large scattering particles with a low refractive index increment (mu).
openaire +2 more sources
Bacteriophage purification by gel chromatography
Analytical Biochemistry, 1981Abstract We have developed an inexpensive procedure for bacteriophage purification suitable for small- and medium-scale preparations (up to one liter of lysate). The method consists of precipitation with polyethylene glycol 6000 and gel chromatography on a Bio-Gel A-5m column. The purity of the phage preparation is comparable to that obtained by CsCl
B, Sain, S, Erdei
openaire +2 more sources
Gel chromatography of carbohydrates on polysaccharide gel
Journal of Polymer Science: Polymer Symposia, 1980AbstractLiquid chromatographies of high and low‐molecular carbohydrates and polyethyleneglycols of different molecular masses were studied on porous microspherical cellulose. Dependence of the distribution coefficients of polymer molecules on molecular mass was determined.
Yu. A. Eltekov +4 more
openaire +1 more source
Fluorinated gels for gel permeation chromatography
Die Makromolekulare Chemie, 1993AbstractThe aim of these investigations was the development of stationary phases for GPC compatible with fluorinated solvents. The properties of crosslinked copolymers of 4‐(2‐heptafluoropropoxy‐1, 2,2‐trifluoroethoxy)styrene (1) were investigated in dependence of the mode of synthesis. Divinylbenzene (DVB) was used as crosslinking agent.
Hans‐Jürgen Juhl, Walter Heitz
openaire +1 more source
Angewandte Chemie International Edition in English, 1970
AbstractGel chromatography can be regarded as a network‐limited partitioning of substances. The theory of this method and the preparation and properties of various gel systems are reviewed. Optimization of the method is illustrated for the separation of oligomers.
openaire +1 more source
AbstractGel chromatography can be regarded as a network‐limited partitioning of substances. The theory of this method and the preparation and properties of various gel systems are reviewed. Optimization of the method is illustrated for the separation of oligomers.
openaire +1 more source
Determination of the structure of agarose gels by gel chromatography
Biochimica et Biophysica Acta (BBA) - General Subjects, 1967Abstract Well-characterized fractions of the synthetic polysaccharide Ficoll have been chromatographed on columns of pearl-condensed agarose gels of concentrations between 2 and 8%. The results agree with the theory that the Ficoll is sterically excluded from the gel, which is made up of a random three-dimensional network of long fibers.
openaire +2 more sources