Results 31 to 40 of about 741,703 (306)

Purification of alpha-toxin from Staphylococcus aureus and application to cell permeabilization [PDF]

, 1987
Crude alpha-toxin was produced by Staphylococcus aureus, strain Wood 46. The amount of exotoxin was monitored during growth and all subsequent purification steps by determination of its hemolytic activity against rabbit erythrocytes.
Ahnert-Hilger, Ahnert-Hilger, Bader, Bhakdi, Bhakdi, Bhakdi, Cassidy, Dunn, Füssle, Laemmli, Lowry, McEwen, Möllby, Reynolds, Thelestam, Wadström, Wilson   +16 more
core   +1 more source

Isolation and Characterization of a Phaseolus vulgaris Trypsin Inhibitor with Antiproliferative Activity on Leukemia and Lymphoma Cells

Molecules, 2017
A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. “gold bean” with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange
Miao Li, Qin Liu, Yajuan Cui, Dong Li, Hexiang Wang, Tzi Bun Ng   +5 more
doaj   +1 more source

Desalination effect on FⅧ components: a compartive study among 5 desalination methods

Zhongguo shuxue zazhi
Objective To compare the desalination effects of five desalination methods and their effects on the components for human coagulation factor Ⅷ(FⅧ), and provide reference for selection of protein desalination methods.
Renjun PEI, Xi DU, Pan SUN, Xiaoyue LI, Peng JIANG, Changqing LI, Fangzhao LIN, Haijun CAO   +7 more
doaj   +1 more source

Evaluation of gel chromatography for plasma lipoprotein fractionation

Journal of Lipid Research, 1970
The fractionation of lipoproteins of normal and hyperlipidemic subjects on a column of 2% agarose was compared with ultracentrifugation and paper electrophoresis procedures. The following results were obtained.
Teizo Sata, David L. Estrich, Peter D.S. Wood, Laurance W. Kinsell   +3 more
doaj   +1 more source

In pursuit of β-amino α-nitro β-trifluoromethyl ketones: nitro-Mannich vs Mannich-type reactions [PDF]

, 2017
The reactivity of alfa-nitro ketones with trifluoromethyl aldimines is studied for the first time. While under nitro-Mannich conditions only the facial stereoselectivity can be controlled, organocatalysed Mannich-type reactions allowed a complete control
Ballini, Ballini, Ballini, Ballini, Bhadury, Carroccia, Carroccia, Chen, Claridge, Córdova, Dighe, Dinér, Donslund, Fioravanti, Fioravanti, Fioravanti, Fioravanti, Fioravanti, Gao, Ghosh, Hatano, Hurd, Ibrahem, Ilies, Kano, Kano, Kelly, Kobayashi, Kokotos, Levy, Merino, Mitsumori, Moteki, Nguyen, Nie, Noble, Parise, Parise, Pelagalli, Stach, Walaszek, Yang, Yin, Zhang, Zhang, Zheng   +45 more
core   +1 more source

Purification and identification of a novel protein isolated from Panax quinquefolium and evaluation of its In vitro antioxidant properties

World Journal of Traditional Chinese Medicine, 2019
Objective: A novel protein was first purified from Panax quinquefolius L. (AGNP), and in vitro antioxidant activities of AGNP were first studied in this work.
Xue-Hui Li, Yang Yang, Zhao Yu, Ning Xu, Xiao-Lei Tang, Xue-Yuan Bai, Si-Ming Wang, Hao-Yuan Chen, Da-Qing Zhao, Li Liu, Bin Qi   +10 more
doaj   +1 more source

Gel filtration chromatography [PDF]

, 2011
Gel-filtration chromatography is a popular and versatile technique that permits the effective separation of proteins and other biological molecules in high yield. Here, the basis of the method is described and typical matrix types are contrasted.
Cummins, Phil, O'Connor, Brendan, Ó Fágáin, Ciarán   +2 more
core  

Structural insights into lacto‐N‐biose I recognition by a family 32 carbohydrate‐binding module from Bifidobacterium bifidum

FEBS Letters, EarlyView.
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang, Naoki Sunagawa, Toma Kashima, Kiyohiko Igarashi, Akimasa Miyanaga, Shinya Fushinobu   +5 more
wiley   +1 more source

Derivatization-free gel permeation chromatography elucidates enzymatic cellulose hydrolysis

Biotechnology for Biofuels, 2012
Background The analysis of cellulose molecular weight distributions by gel permeation chromatography (GPC) is a powerful tool to obtain detailed information on enzymatic cellulose hydrolysis, supporting the development of economically viable biorefinery ...
Engel Philip, Hein Lea, Spiess Antje C
doaj   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source