Results 41 to 50 of about 1,439,714 (301)

Analytical gel filtration chromatography.

, 2016
Analytical gel filtration chromatography of BcGR was performed in the absence of substrates (solid line) and in the presence of 10 mM D-Glu (dashed line) or L-glutamate (dotted line). The inset represents the molecular weight calibration curve, performed
Silvia Buroni (65276), Viola Camilla Scoffone (3369119), Aygun Israyilova (3369116), Federico Forneris (468673), Laurent Roberto Chiarelli (237923), Giovanna Riccardi (65279), Namiq Q. Shixaliyev (3369113)   +6 more
core   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

Oxalate-silica gel thin-layer system for free 2-hydroxy fatty acids and for fatty acyl coenzyme A

Journal of Lipid Research, 1972
The 2-hydroxy fatty acids tend to yield streaks in thin-layer chromatography on silica gel plates. If potassium oxalate is included with binder-free silica gel, good spots are obtained.
M. David Ullman, Norman S. Radin
doaj   +1 more source

Isolation of leptin hormone from plasma and molecular weight estimation using bio techniques [PDF]

مجلة التربية والعلم, 2009
The research include the isolation of leptin hormone from plasma of female by gel filtration chromatography technique using separating column 1002Cm containing Sephadex G - 75 produced 5 bands where number 5 showed leptin concentration 0.5ng/ml.
Nawal Thnoon Youis, Qusi Al Jalabi, Thikra Ali   +2 more
doaj   +1 more source

Test No. 119: Determination of the Low Molecular Weight Content of a Polymer Using Gel Permeation Chromatography [electronic resource] /

, 1996
This Test guideline describes the Gel Permeation Chromatography (GPC). This method permits to determine the molecular weight distribution and the average molecular weights (Mn, Mw).
Organisation for Economic Co-operation and Development., null null   +1 more
core   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Purification of the Rv0045c protein by ion exchange chromatography and gel filtration chromatography.

, 2013
The Rv0045c protein was purified by anion exchange chromatography (A), cation exchange chromatography (B), and gel filtration chromatography (C). The purity was checked by SDS-PAGE analysis after each purification procedure.
Kehui Xu (361209), Siguo Liu (103949), Hai Pang (102853), Jiubiao Guo (350857), Zhongyuan Liu (361206), Xiangdong Zheng (350856), Lipeng Xu (350858), Shentao Li (361213), Tingyi Wen (10249)   +8 more
core   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, Volume 20, Issue 11, Page 2876-2895, November 2026.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Regenerated Silica Gel for Isolation of Ethyl p-Methoxycinnamate from Kaempferia galanga rhizome [PDF]

E3S Web of Conferences
The main component from Kaempferia galanga rhizome extract is known as ethyl p-methoxycinnamate. Column chromatography using silica gel is one of the commonly used method for obtaining high purity of ethyl p-methoxycinnamate from K.
Mazaya Nadia Adelia, Widiastuti Diana, yati Indri, Prastya Muhammad Eka, Andreani Agustina Sus, Tachrim Zetryana Puteri   +5 more
doaj   +1 more source

Isolation and purification of copeptin hormone from serum of patients with heart disease

Tikrit Journal of Pure Science, 2023
This study included isolation and purification of copepin hormone from heart disease patients serum using different biochemical techniques. The study showed two proteinous peak has been isolated by gel filtration (Sephadex G-50) from the precipitation ...
Thikra Ali Allwsh, Noori Mohammed Aziz
doaj   +1 more source