Results 31 to 40 of about 1,439,714 (301)

Isolation and Characterization of a Phaseolus vulgaris Trypsin Inhibitor with Antiproliferative Activity on Leukemia and Lymphoma Cells

Molecules, 2017
A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. “gold bean” with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange
Miao Li, Qin Liu, Yajuan Cui, Dong Li, Hexiang Wang, Tzi Bun Ng   +5 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Purification and identification of a novel protein isolated from Panax quinquefolium and evaluation of its In vitro antioxidant properties

World Journal of Traditional Chinese Medicine, 2019
Objective: A novel protein was first purified from Panax quinquefolius L. (AGNP), and in vitro antioxidant activities of AGNP were first studied in this work.
Xue-Hui Li, Yang Yang, Zhao Yu, Ning Xu, Xiao-Lei Tang, Xue-Yuan Bai, Si-Ming Wang, Hao-Yuan Chen, Da-Qing Zhao, Li Liu, Bin Qi   +10 more
doaj   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

Derivatization-free gel permeation chromatography elucidates enzymatic cellulose hydrolysis

Biotechnology for Biofuels, 2012
Background The analysis of cellulose molecular weight distributions by gel permeation chromatography (GPC) is a powerful tool to obtain detailed information on enzymatic cellulose hydrolysis, supporting the development of economically viable biorefinery ...
Engel Philip, Hein Lea, Spiess Antje C
doaj   +1 more source

VGF C-terminus fragments found by gel chromatography in human.

, 2016
VGF C-terminus fragments found by gel chromatography in human.
Roberta Puddu (3219888), Filomena D’Amato (639525), Barbara Manconi (367243), Alessandro Vercelli (139469), Sandro Orrù (844449), Carla Brancia (639524), Irene Messana (333885), Marina Boido (306249), Barbara Noli (639523), Federica Vincenzoni (3219894), Gian-Luca Ferri (316242), Paolo Bongioanni (3219891), Andrea Boi (3219900), Giuseppe Borghero (3219897), Francesco Marrosu (304911), Cristina Cocco (639526)   +15 more
core   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

Dry-column chromatographic isolation of fatty acid esters of phorbol from croton oil

Journal of Lipid Research, 1969
The family of mixed fatty acid esters of 4,9,12,-13,20-pentahydroxy-tiglia-1,6-dien-3-one (phorbol) has been isolated from croton oil by column chromatography on silica gel and preparative dry-column chromatography on silica gel HF/254 packed in a ...
Paul R. Ocken
doaj   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Laboratory Database to Manage Electrophoresis and Chromatography Separations and the Associated Samples

BioTechniques, 2000
A database was developed to store, organize, and retrieve the data associated with electrophoresis and chromatography separations. It allows laboratories to store extensive data on separation techniques (analytical and preparative).
Kenneth D. Cole
doaj   +1 more source