Results 61 to 70 of about 3,285,010 (339)

Implementation of Multidimensional Protein Identification Technology and its Application to the Characterization of Protein Complexes in Bakers Yeast [PDF]

, 2006
The analysis of complex polypeptide mixtures poses a central and ubiquitous problem to biochemistry, molecular and cellular biology. Historically the problem has been approached by means of gel electrophoretic separation, coupled to immune-chemistry or ...
Graumann, Johannes
core   +1 more source

Preparation, chemical structure, and immunostimulatory activity of a water-soluble heteropolysaccharide from Suillus granulatus fruiting bodies

Food Chemistry: X, 2022
A water-soluble heteropolysaccharide (SGP2-1) was purified from Suillus granulatus fruiting bodies by anion-exchange chromatography and gel permeation chromatography.
Xiong Gao, Ranhua Zeng, Chi-Tang Ho, Bin Li, Shaodan Chen, Chun Xiao, Huiping Hu, Manjun Cai, Zhongzheng Chen, Yizhen Xie, Qingping Wu   +10 more
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Liquid-gel chromatography on lipophilic-hydrophobic Sephadex derivatives

Journal of Lipid Research, 1970
Hydrophobic long-chain alkyl ethers of Sephadex have been synthesized and tested for use in liquid chromatography. With columns prepared in such polar solvents as methanol, reversed-phase systems are obtained where compounds separate in order of ...
J. Ellingboe, E. Nyström, J. Sjövall
doaj   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Screening, Enrichment and Structural Identification of Bitter Almond Antibacterial Peptides

Shipin gongye ke-ji
To explore the preparation, enrichment, and structural analysis of bitter almond antimicrobial peptides, in this experiment, the protein was extracted from the defatted bitter almonds, and the protein was hydrolyzed by enzymes to prepare bitter almond ...
Tao ZHANG, Jie MA, Guofang NIAN, Zhiyi ZHOU, Ting ZHANG, Jianzhong ZHOU   +5 more
doaj   +1 more source

Studies on the chemical compositions of Hyptis suaveolens (L.) Poit [PDF]

Journal of the Serbian Chemical Society, 2019
This study aimed at identifying the chemical compounds isolated from the aerial parts and roots of Hyptis suaveolens (L.) Poit. The compounds were isolated and purified by a combination of various chromatographic techniques including silica gel column ...
Tang Gengqiu, Liu Xile, Gong Xue, Lin Xiaojing, Lai Xiudi, Wang Dong, Ji Shengguo   +6 more
doaj   +1 more source

A novel quinazolinone insulin receptor inhibitor and its synergy with an EGFR inhibitor in glucose‐driven glioblastoma

Molecular Oncology, EarlyView.
The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka, Wioleta Cieślik, Wojciech Płaziński, Katarzyna Stępnik, Anna Boguszewska‐Czubara, Elżbieta Kot, Robert Musioł, Mateusz Jasica, Anna Mrozek‐Wilczkiewicz, Katarzyna Malarz   +9 more
wiley   +1 more source

Simultaneous determination of jasmonic acid epimers as phytohormones by chiral liquid chromatography-quadrupole time-of-flight mass spectrometry and their epimerization study

, 2012
Jasmonic acid (JA) is an essential plant hormone involved in plant development and defense system. There are four stereoisorneric forms of JA and they act quite differently in vivo.
Wu, Hongliang, Liu, Huwei, Han, Yehua, Bai, Yu, Nie, Honggang, Zhou, Zhigui, Lei, Rong   +6 more
core   +1 more source

Identification of bile alcohols in urine from healthy humans.

Journal of Lipid Research, 1984
Bile alcohols present in urine from healthy adults were studied. Urine was extracted with octadecylsilane-bonded silica gel, and a neutral fraction and a glucuronide fraction were isolated by ion exchange chromatography on piperidinohydroxypropyl ...
M Kuwabara, T Ushiroguchi, K Kihira, T Kuramoto, T Hoshita   +4 more
doaj   +1 more source