Results 71 to 80 of about 444,530 (310)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Desenvolvimento de metodologia em sistema em fluxo para especiação e determinação de cromo em água usando sílica gel modificada e espectrometria de absorção atômica em chama [PDF]

, 2003
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Físicas e Matemáticas. Programa de Pós-graduação em Química.Este trabalho descreve o desenvolvimento de um sistema de pré-concentração em fluxo das espécies Cr (III) e Cr
Maltez, Heloisa França
core  

Purification of mRANKL by affinity chromatography and gel filtration chromatography. [PDF]

, 2014
A.SDS-PAGE analysis of purified mRANKL. The fusion mRANKL was cleaved by Factor Xa, purified by affinity chromatography followed by gel filtration chromatography. Eluted proteins fractions (Lane 2–7) were checked by SDS-PAGE gel.
Yun-Jaie Choi (49981), Bijay Singh (561296), Jin-Duck Bok (561297), Tao Jiang (28401), Jeong-In Kim (561298), Chong-Su Cho (49982), Sushila Maharjan (561295), Sang-Kee Kang (78925)   +7 more
core   +1 more source

Separation of lipids by Silica Gel G column chromatography

Journal of Lipid Research, 1964
A column chromatographic procedure utilizing Silica Gel G is described for separating lipid components of serum and lipoproteins into individual fractions containing hydrocarbons (I), cholesterol esters (II), triglycerides (III), cholesterol (IV), free ...
Quincy E. Crider, Petar Alaupovic, Joe Hillsberry, Cathy Yen, Reagan H. Bradford   +4 more
doaj   +1 more source

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

FEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey, Vaishnavi Sanjayan, Praveen Singh, Shantanu Sengupta, Anand Kumar Bachhawat   +4 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Purification of rat pancreatic lipase

Journal of Lipid Research, 1968
A procedure for the isolation of lipase (glycerolester hydrolase, EC 3.1.1.3) from rat pancreas is described. The purification scheme includes homogenization of the pancreas. centrifugation at 3,000 rpm, centrifugation at 40,000 rpm.
Lewis I. Gidez
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Development and characterisation of monolithic silica stationary phases for ion chromatography [PDF]

, 2006
Silica based monolithic HPLC columns have received much attention in recent years, particularly those modified for reversed-phase separations. However, for ionexchange, silica based monolithic columns have received only limited attention.
Sugrue, Edel
core  

Gel filtration column chromatography of calf serum. [PDF]

, 2014
(A) Elution profile of gel filtration column chromatography using a Superdex 200. Open circles indicate diameters of colonies. Filled circles indicate absorbance at 280 nm. Molecular weight markers were eluted in the fraction described below.
Yosuke Omae (230962), Chikara Kaito (230958), Kazuhisa Sekimizu (206633)   +2 more
core   +1 more source