Results 81 to 90 of about 3,285,010 (339)
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
FEBS Open Bio, EarlyView.The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
Desenvolvimento de metodologia em sistema em fluxo para especiação e determinação de cromo em água usando sílica gel modificada e espectrometria de absorção atômica em chama [PDF]
, 2003 Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Físicas e Matemáticas. Programa de Pós-graduação em Química.Este trabalho descreve o desenvolvimento de um sistema de pré-concentração em fluxo das espécies Cr (III) e Cr
Maltez, Heloisa França
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Purification of a 19-kDa pore-forming cytolysin from the sea anemone Heteractis magnifica
Journal of Venomous Animals and Toxins including Tropical Diseases, 2010 Pore-forming cytolysins of 19 kDa from sea anemones present a remarkable cytolytic property. In the present work, a purified 19-kDa cytolysin was obtained from the sea anemone Heteractis magnifica.
S Karthikayalu, V Rama, R Venkatesan
doaj +1 more source
Application of gel chromatography [PDF]
Pure and Applied Chemistry, 1969 Abstract
openaire +1 more source
FEBS Open Bio, EarlyView.In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
Desenvolvimento e aplicação de fases extratoras para técnicas de preparação de amostras [PDF]
, 2009 Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Físicas e Matemáticas. Programa de Pós-Graduação em Químca.A fragilidade da sílica fundida e a necessidade de suportes robustos para microextração em fase sólida (SPME) têm ...
Budziak, Dilma
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Journal of Lipid Research, 1979 A method for separating individual monosulfated primary bile acid conjugates by reversed-phase partition thin-layer chromatography on octadecyl-bonded silica gel is described.
R Raedsch, A F Hofmann, K Y Tserng
doaj +1 more source
A minimal cellulosome‐like system in Cellulosilyticum lentocellum
FEBS Open Bio, EarlyView.Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan +2 more
wiley +1 more source
The Molecular Weight Characterisation of Pinus radiata Bark Condensed Tannins [PDF]
, 2007 Crude condensed tannins extracted from Pinus radiata bark (sourced from Tokoroa) with various solvents (Hot water (HW), methanol/water/acetic acid (MWA), acetone/water (AW) and hot aqueous 2% sulphite/urea solution (SFU)) were subjected to Sephadex LH-20
Bogun, Ben Rowan
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