Preparative gel electrophoresis of double- stranded DNA usually includes staining the gel with ethidium bromide followed by illumination with ultraviolet (UV-B) light. In this report, DNA isolated from agarose gels was found to be a poor substrate for in
Dirk Gründemann, Edgar Schömig
doaj +1 more source
A versatile electrophoresis system for the analysis of high- and low-molecular-weight proteins [PDF]
A new, versatile, multiphasic buffer system for high-resolution sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins in the relative molecular weight range of 300 000-3000 Da is described. The system, based on the theory of multiphasic zone electrophoresis, allows complete stacking and destacking of proteins in the above M(r) range ...
arxiv
CNS Mitochondria‐Derived Vesicle in Blood: Potential Biomarkers for Brain Mitochondria Dysfunction
ABSTRACT Objective Mitochondrial dysfunction is a hallmark of neurodegenerative diseases like Alzheimer's (AD) and Parkinson's (PD). Our goal was to develop practical, noninvasive methods to assess mitochondrial status through the detection of mitochondria‐derived vesicles (MDVs).
Qi Liu+12 more
wiley +1 more source
Agarose gel electrophoresis: Applications in clinical chemistry [PDF]
Agarose gel electrophoresis is a well established technique routinely used in clinical laboratories for screening protein abnormalities in various biological fluids (serum, urine, CSF).
Giot Jean-Francois
doaj
Gel-eletroforese no diagnóstico da varíola Gel-electrophoresis in the smallpox diagnosis
O emprego de gel-eletroforese no diagnóstico da varíola, demonstrou ser ao menos trinta vezes (30X) mais sensível que o teste de agar-gel, nas condições descritas (tabela I).
Julio A. Mesquita+2 more
doaj +1 more source
Bioorthogonal Engineering of Cellular Microenvironments Using Isonitrile Ligations
Highly selective chemistries are required for fabrication and post‐cross–linking modification of cell‐encapsulating hydrogels used in tissue engineering applications. Isonitrile ligation reactions represent a promising class of bioorthogonal chemistries for engineering hydrogel‐based cellular microenvironments. Isonitrile‐based hydrogels are stable and
Ping Zhou+2 more
wiley +1 more source
Cell Membrane Vesicle Camouflaged Artificial Cells
Artificial cells camouflaged with a cell membrane vesicle coating are able to assemble into synthetic aggregates that exhibit rudimentary communication capabilities. Additionally, when these artificial cells are equipped with antioxidant capabilities, they are able to protect the intracellular homeostasis in HepG2 cells present in semi‐synthetic ...
Paula De Dios Andres+11 more
wiley +1 more source
An improved procedure for starch-gel electrophoresis: further variations in the serum proteins of normal individuals [PDF]
Oliver Smithies
openalex +1 more source
A Strong and Water‐Retaining Biomass Adhesive Inspired by Tofu
Drawing inspiration from the formation mechanism of the traditional food tofu, a strong and water‐retaining adhesive is designed using the soybean meal (SM) oxidized by glucose oxidase (GOx) and calcium sulfate oligomer (CSO). This design strategy effectively addresses the conflicting requirements of water‐resistant bonding strength and water retention
Jiawei Shao+8 more
wiley +1 more source
Preparation of thin PbTiO3 Nanotubes by the Electrophoretic Deposition Method [PDF]
Sol-gel electrophoresis is used to grow PbTiO3 nanotube arrays in porous anodic alumina template channels, because it is a cheap and simple method for the growth of nanostructures and has the advantage of better tube growth control. Moreover, this method can produce nanotubes with high quality and more condense structures.
arxiv