Results 161 to 170 of about 2,044,735 (395)

TPMS‐Gyroid Scaffold‐Mediated Up‐Regulation of ITGB1 for Enhanced Cell Adhesion and Immune‐Modulatory Osteogenesis

open access: yesAdvanced Healthcare Materials, EarlyView.
A) SLM generates biomimetic bone scaffolds with consistent porosity but varying TPMS‐Gyroid unit cell designs (TG15, TG20, TG25, TG30). B) By enhancing the expression of ITGB1, TPMS‐Gyroid scaffolds can facilitate osteogenic differentiation in BMSCs and promote M2 polarization in macrophages.
Jing Wang   +9 more
wiley   +1 more source

Sequence-specific double-strand cleavage of DNA by penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [PDF]

open access: yes, 1983
In the presence of O2 and 5 mM dithiothreitol, penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [P5E·Fe(II)] at 0.5 µ M cleaves pBR322 plasmid DNA (50 µ M in base pairs) on opposite strands to afford discrete DNA fragments as analyzed by agarose gel ...
Dervan, Peter B., Schultz, Peter G.
core  

Porous Silicon Nanoneedles Efficiently Deliver Adenine Base Editor to Correct a Recurrent Pathogenic COL7A1 Variant in Recessive Dystrophic Epidermolysis Bullosa

open access: yesAdvanced Materials, EarlyView.
Base editing using nanoneedles efficiently corrects pathogenic variants in the type VII collagen gene of primary fibroblasts from patients with recessive dystrophic epidermolysis bullosa. Nanoneedle editing induces minimal and reversible transcriptional perturbations while restoring the production and secretion of functional type VII collagen.
Salman Ahmad Mustfa   +10 more
wiley   +1 more source

Fluorescent Visualization of In Vitro Mitochondrial DNA Transcription [PDF]

open access: yes, 2019
Mitochondria are important organelles within eukaryotic cells especially for their role in metabolism and ATP production by the oxidative phosphorylation (OXPHOS) pathway. In human cells there are approximately 80 protein subunits that make up the OXPHOS
Bruce, Kelsey
core   +1 more source

Hybrid DNA Origami – Graphene Platform for Electrically‐Gated Nanoscale Motion

open access: yesAdvanced Materials Interfaces, EarlyView.
A hybrid DNA origami‐graphene architecture is employed in a nearfield sensing device for optical distance assessment in biosensors and biorecognition purposes. Using Fluorescence Lifetime Imaging Microscopy (FLIM), a nearfield quenching model is verified and test the device response under several electrostatic gating conditions, which leads to changes ...
João D. G. Azevedo   +10 more
wiley   +1 more source

A Sustainable Route From Quartz to Bifunctional Material with Adsorbed Lanthanides for Enhanced Fluorescent Activation in Doxycycline Sensing

open access: yesAdvanced Materials Interfaces, EarlyView.
The aim of this study is to develop a sensor material based on natural quartz combined with La(III) and Ce(III), both detected in Ni‐MH batteries, as well as Eu(III), for reliable qualitative and quantitative analysis of doxycycline over a broad concentration range.
Olha Semeshko   +6 more
wiley   +1 more source

Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients

open access: yesBrazilian Journal of Infectious Diseases
Burkholderia cenocepacia may cause serious infections in patients with cystic fibrosis, and this microorganism can be highly transmissible. Pulsed-field gel electrophoresis is widely used to study the dynamics of strain spread in cystic fibrosis patients.
Luana Pretto   +4 more
doaj   +1 more source

Two Dimensional Electrophoresis of Galactosidase Relating to the Disappearance of Bombyx Lectin Activity [PDF]

open access: yes, 2004
"Two dimensional polyacrylamide gel electroporesis (2 D-PAGE) analysis on the haemolymph of Bombyx mori was performed using the Mini-PROTEAN mini tube gel two dimensional polyacrylamide gel electrophoresis system (Bio-Rad Laboratories, Inc.).
Yasuo Kato, カトウ ヤスオ
core   +1 more source

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