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Polyacrylamide Gel Electrophoresis
Science, 1971Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, measurable pore size over ...
A, Chrambach, D, Rodbard
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Polyacrylamide Gel Electrophoresis
Cold Spring Harbor Protocols, 2020Cross-linked chains of polyacrylamide can be used as electrically neutral gels to separate double-stranded DNA fragments according to size and single-stranded DNAs according to size and conformation. Polyacrylamide gels have the following three major advantages over agarose gels: (1) Their resolving power is so great that they can separate molecules of
Michael R, Green, Joseph, Sambrook
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Cold Spring Harbor Protocols, 2019
This protocol describes steps for the preparation and running of agarose gels and for staining and visualization of DNA in gels using three dyes: ethidium bromide, SYBR Gold, and SYBR Green 1.
Michael R, Green, Joseph, Sambrook
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This protocol describes steps for the preparation and running of agarose gels and for staining and visualization of DNA in gels using three dyes: ethidium bromide, SYBR Gold, and SYBR Green 1.
Michael R, Green, Joseph, Sambrook
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Carbohydrate Gel Electrophoresis
2010Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) relies on derivatization of reducing ends of sugars with a fluorophore, followed by electrophoresis under optimized conditions in polyacrylamide gels. PACE is a sensitive and simple tool for studying polysaccharide structure or quantity and also has applications in the investigation ...
Goubet, Florence +2 more
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Nanocellulose for gel electrophoresis
Journal of Colloid and Interface Science, 2019Cellulose nanofibres produced by TEMPO-mediated oxidation can form gels. This study presents a proof-of-concept for gel electrophoresis with nanocellulose (NC).TEMPO-oxidised cellulose nanofibre dispersion is chemically cross-linked by inducing amide linkages to produce gel slabs for electrophoretic separation.
Llyza, Mendoza +3 more
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Difference gel electrophoresis
PROTEOMICS, 2008AbstractDIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS‐based methodologies and can circumvent their analytical ...
John F, Timms, Rainer, Cramer
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The gel electrophoresis of DNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972Abstract 1. 1. We have compared the electrophoretic mobility of linear duplex DNAs (bacteriophages T4, lambda, T7 and Φ29) and of circular duplex DNAs (replicative form DNA of bacteriophage ΦX174, bacteriophage PM 2 DNA and rat mtDNA) in agarose gels.
C, Aaij, P, Borst
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2003
After digestion of DNA with a restriction enzyme (Chapter 50), it is usually necessary, for both preparative and analytical purposes, to separate and visualize the products. In most cases, where the products are between 200 and 20,000 bp long, this is achieved by agarose gel electrophoresis.
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After digestion of DNA with a restriction enzyme (Chapter 50), it is usually necessary, for both preparative and analytical purposes, to separate and visualize the products. In most cases, where the products are between 200 and 20,000 bp long, this is achieved by agarose gel electrophoresis.
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Scandinavian Journal of Clinical and Laboratory Investigation, 1972
A simple technique for agarose gel electrophoresis allowing the simultaneous separation of 15 samples in less than one hour is described.
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A simple technique for agarose gel electrophoresis allowing the simultaneous separation of 15 samples in less than one hour is described.
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Current Protocols in Immunology, 1991
AbstractDiagonal gel electrophoresis is a form of two‐dimensional analysis useful for investigating the subunit composition of multisubunit proteins containing interchain disulfide bonds. Proteins are electrophoresed in the first dimension in a slab or tube gel under nonreducing conditions. The proteins are then reduced in the gel and this piece of gel
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AbstractDiagonal gel electrophoresis is a form of two‐dimensional analysis useful for investigating the subunit composition of multisubunit proteins containing interchain disulfide bonds. Proteins are electrophoresed in the first dimension in a slab or tube gel under nonreducing conditions. The proteins are then reduced in the gel and this piece of gel
openaire +2 more sources