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2013
Various types of electrophoretic separations are commonly used in proteomic approach. This chapter describes principle of electrophoresis in agarose, polyacrylamide, isoelectrofocusing, and 2-dimensional separations. Separations in native or denaturing conditions are discussed.
Jerzy Silberring +2 more
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Various types of electrophoretic separations are commonly used in proteomic approach. This chapter describes principle of electrophoresis in agarose, polyacrylamide, isoelectrofocusing, and 2-dimensional separations. Separations in native or denaturing conditions are discussed.
Jerzy Silberring +2 more
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Clinica Chimica Acta, 1967
Abstract A modificed disc electrophoresis method is described in detail. The gel columns were made in tubes of 0.9 mm internal diameter. The method requires a total protein content of 0.01 mg. Samples containing 80–100 mg protein per 100 ml can be studied without concentration. Resolution is as good as in the original macro method.
V. Raunio, U. Krause
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Abstract A modificed disc electrophoresis method is described in detail. The gel columns were made in tubes of 0.9 mm internal diameter. The method requires a total protein content of 0.01 mg. Samples containing 80–100 mg protein per 100 ml can be studied without concentration. Resolution is as good as in the original macro method.
V. Raunio, U. Krause
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Difference gel electrophoresis DIGE [PDF]
Drug Discovery Today: Technologies, 2006 Proteomics offers powerful technologies to assist in the discovery of targets for novel therapeutic agents, by allowing the investigation of changes in protein state between control and diseased tissue and biofluids. Difference gel electrophoresis coupled with mass spectrometry (DIGE/MS) is a technology used within proteomics that has demonstrated ...
Kathryn S. Lilley, David B. Friedman
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Difference gel electrophoresis
PROTEOMICS, 2008AbstractDIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS‐based methodologies and can circumvent their analytical ...
Rainer Cramer, John F. Timms
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2003
After digestion of DNA with a restriction enzyme (Chapter 50), it is usually necessary, for both preparative and analytical purposes, to separate and visualize the products. In most cases, where the products are between 200 and 20,000 bp long, this is achieved by agarose gel electrophoresis.
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After digestion of DNA with a restriction enzyme (Chapter 50), it is usually necessary, for both preparative and analytical purposes, to separate and visualize the products. In most cases, where the products are between 200 and 20,000 bp long, this is achieved by agarose gel electrophoresis.
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Scandinavian Journal of Clinical and Laboratory Investigation, 1972
A simple technique for agarose gel electrophoresis allowing the simultaneous separation of 15 samples in less than one hour is described.
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A simple technique for agarose gel electrophoresis allowing the simultaneous separation of 15 samples in less than one hour is described.
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ELECTROPHORESIS, 1983
AbstractA miniature system for two‐dimensional polyacrylamide gel electrophoresis is presented. Discontinuous sodium dodecyl sulfate gel electrophoresis in micro‐slab gels following high resolution micro‐electrophoresis in cylindrical continuous polyacrylamide gradient gels separates macromolecules after an initial size fractionation into their ...
Sabine Zimmermann +3 more
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AbstractA miniature system for two‐dimensional polyacrylamide gel electrophoresis is presented. Discontinuous sodium dodecyl sulfate gel electrophoresis in micro‐slab gels following high resolution micro‐electrophoresis in cylindrical continuous polyacrylamide gradient gels separates macromolecules after an initial size fractionation into their ...
Sabine Zimmermann +3 more
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Trends in Biochemical Sciences, 2000
Work at Brookhaven National Laboratory during the period described in the article was supported by the US Atomic Energy Commission, a predecessor to our current sponsor, the US Dept of Energy.
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Work at Brookhaven National Laboratory during the period described in the article was supported by the US Atomic Energy Commission, a predecessor to our current sponsor, the US Dept of Energy.
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Electrophoresis and Gel Strength
Nature, 1954A CURIOUS phenomenon has been noted in recent work on the electrophoresis of sodium carrageenate. This polysaccharide occurs in the red seaweed, Chondrus crispus, and is composed mainly of d-galactose sulphate units linked in the 1–3 positions. Cations associated with the sulphate can become ionized to make the molecule a polyelectrolyte.
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Pulsed-field gel electrophoresis [PDF]
Molecular Biotechnology, 1998 Pulsed-field gel electrophoresis (PFGE) was originally developed as a technique for providing electrophoretic karyotypes of micro-organisms. Since then the technique has evolved and diversified in many new directions. This review traces the evolution of PFGE, summarizes our understanding of its theoretical basis, and provides a comprehensive ...
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