Results 21 to 30 of about 1,126,750 (353)

Conducting polymer electrodes for gel electrophoresis.

open access: yesPLoS ONE, 2014
In nearly all cases, electrophoresis in gels is driven via the electrolysis of water at the electrodes, where the process consumes water and produces electrochemical by-products. We have previously demonstrated that π-conjugated polymers such as poly(3,4-
Katarina Bengtsson   +2 more
doaj   +1 more source

How to use 2D gel electrophoresis in plant proteomics [PDF]

open access: yesMethods in Molecular Biology -Clifton then Totowa- 1072 (2014) 43-50, 2013
Two-dimensional electrophoresis has nurtured the birth of proteomics. It is however no longer the exclusive setup used in proteomics, with the development of shotgun proteomics techniques that appear more fancy and fashionable nowadays.Nevertheless, 2D gel-based proteomics still has valuable features, and sometimes unique ones, which make it often an ...
arxiv   +1 more source

Use of capillary electrophoresis as a method development tool for classical gel electrophoresis [PDF]

open access: yes, 1998
Capillary electrophoresis (CE) was used to optimize the buffer pH, ionic strength and sulfated cyclodextrin concentrations for enantiomeric separation of piperoxan.
Gratz, Samuel R.   +2 more
core   +1 more source

ELECTROPHORETIC SYSTEMS FOR PREPARATIVE FRACTIONATION OF PROTEIN PRECURSORS OF BIOACTIVE PEPTIDES FROM COW’S MILK

open access: yesHarčova Nauka ì Tehnologìâ, 2018
The article considers the possibility of obtaining purified fractions-precursors of bioactive peptides from milk proteins by the method of preparative electrophoresis.
V. Yukalo   +3 more
doaj   +1 more source

What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?

open access: yesProteomes, 2020
Two-dimensional gel electrophoresis was instrumental in the birth of proteomics in the late 1980s. However, it is now often considered as an outdated technique for proteomics—a thing of the past.
Katrin Marcus   +2 more
doaj   +1 more source

Gels in Microscale Electrophoresis

open access: yesAnnual Review of Analytical Chemistry, 2023
Gel matrices are fundamental to electrophoresis analyses of biopolymers in microscale channels. Both capillary gel and microchannel gel electrophoresis systems have produced fundamental advances in the scientific community. These analytical techniques remain as foundational tools in bioanalytical chemistry and are indispensable in the field of ...
Lisa A, Holland, Laura D, Casto-Boggess
openaire   +2 more sources

Isolation of Acidic, Basic and Neutral Metalloproteins by QPNC-PAGE [PDF]

open access: yes, 2010
A standard protocol for isolating metalloproteins in complex biological samples is presented using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC ...
Bernd Kastenholz, David E. Garfin
core   +2 more sources

Design Development Research Approach in Developing Gel Electrophoresis Virtual Laboratory to substitute Real Practicum

open access: yesScientiae Educatia: Jurnal Pendidikan Sains, 2019
One of the purposes of Genetic practicum is that students can understand DNA separation techniques, by conducting gel electrophoresis practicum.
Yuyun Maryuningsih   +3 more
doaj   +1 more source

Genomic and proteomic profiling for cancer diagnosis in dogs [PDF]

open access: yes, 2016
Global gene expression, whereby tumours are classified according to similar gene expression patterns or ‘signatures’ regardless of cell morphology or tissue characteristics, is being increasingly used in both the human and veterinary fields to assist in ...
Morris, Joanna S.
core   +1 more source

Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients [PDF]

open access: yesElectrophoresis 18 (31/03/1997) 307-16, 2006
Membrane and nuclear proteins of poor solubility have been separated by high resolution two-dimensional (2-D) gel electrophoresis. Isoelectric focusing with immobilized pH gradients leads to severe quantitative losses of proteins in the resulting 2-D map, although the resolution is usually high.
arxiv   +1 more source

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