Results 21 to 30 of about 1,139,757 (256)

A microcontroller-based software framework for controlling a mechatronic system [PDF]

open access: yesMATEC Web of Conferences, 2020
The proposed software framework is presented and an Application Programming Interface (API) is developed based on the Arduino Mega 2560. The API processes external commands that follow the operational logic of a gel electrophoresis device.
Nikolaidis Nikolaos   +7 more
doaj   +1 more source

ELECTROPHORETIC SYSTEMS FOR PREPARATIVE FRACTIONATION OF PROTEIN PRECURSORS OF BIOACTIVE PEPTIDES FROM COW’S MILK

open access: yesHarčova Nauka ì Tehnologìâ, 2018
The article considers the possibility of obtaining purified fractions-precursors of bioactive peptides from milk proteins by the method of preparative electrophoresis.
V. Yukalo   +3 more
doaj   +1 more source

Design Development Research Approach in Developing Gel Electrophoresis Virtual Laboratory to substitute Real Practicum

open access: yesScientiae Educatia: Jurnal Pendidikan Sains, 2019
One of the purposes of Genetic practicum is that students can understand DNA separation techniques, by conducting gel electrophoresis practicum.
Yuyun Maryuningsih   +3 more
doaj   +1 more source

What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?

open access: yesProteomes, 2020
Two-dimensional gel electrophoresis was instrumental in the birth of proteomics in the late 1980s. However, it is now often considered as an outdated technique for proteomics—a thing of the past.
Katrin Marcus   +2 more
doaj   +1 more source

Rubisco : easy purification and immunochemical determination [PDF]

open access: yes, 2001
Rubisco (Ribulose-1.5-bisphosphate carboxylase/oxygenase) from spinach was purified to homogeneity in one step by gel filtration. This enzyme is suitable for the generation of a specific antibody in rabbits.
Groß, Ulrich
core   +1 more source

Semi-denaturing Detergent Agarose Gel Electrophoresis (SDD-AGE)

open access: yesBio-Protocol, 2014
Pathological proteins in neurodegenerative diseases suffer a conformational change to a misfolded amyloid state. Such pathological event leads to the aggregation of these proteins that indefinitely propagates as an altered form of itself, and harbor ...
Laura Molina-García   +1 more
doaj   +1 more source

Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis

open access: yesJournal of Pharmacopuncture, 2006
Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide ...
Young Chon Choi   +2 more
doaj   +1 more source

Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myelo\"id cell lines nuclear proteomes [PDF]

open access: yes, 2012
One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear proteins lies in their strong interaction with nuclear acids.
Chevallet, Mireille   +5 more
core   +4 more sources

Comparative Proteomics and Difference Gel Electrophoresis

open access: yesBioTechniques, 2007
The goal of comparative proteomics is to analyze proteome changes in response to development, disease, or environment. This is a two-step process in which proteins within cellular extracts are first fractionated to reduce sample complexity, and then the ...
Jonathan Minden
doaj   +1 more source

Analysis of proteins from marine molluscs [PDF]

open access: yes, 2009
Application of the two-dimensional gel electrophoresis (2DE) protocols which were developed for samples of mammalian origin gives unsatisfactory results when used in samples from marine molluscs. This chapter describes a detailed protocol of 2DE that can
Bebianno, Maria João   +2 more
core   +1 more source

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