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Gels in Microscale Electrophoresis
Annual Review of Analytical Chemistry, 2023 Gel matrices are fundamental to electrophoresis analyses of biopolymers in microscale channels. Both capillary gel and microchannel gel electrophoresis systems have produced fundamental advances in the scientific community. These analytical techniques remain as foundational tools in bioanalytical chemistry and are indispensable in the field of ...
Lisa A, Holland, Laura D, Casto-Boggess
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Scientiae Educatia: Jurnal Pendidikan Sains, 2019 One of the purposes of Genetic practicum is that students can understand DNA separation techniques, by conducting gel electrophoresis practicum.
Yuyun Maryuningsih +3 more
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What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?
Proteomes, 2020 Two-dimensional gel electrophoresis was instrumental in the birth of proteomics in the late 1980s. However, it is now often considered as an outdated technique for proteomics—a thing of the past.
Katrin Marcus +2 more
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Use of capillary electrophoresis as a method development tool for classical gel electrophoresis [PDF]
, 1998 Capillary electrophoresis (CE) was used to optimize the buffer pH, ionic strength and sulfated cyclodextrin concentrations for enantiomeric separation of piperoxan.
Gratz, Samuel R. +2 more
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Genomic and proteomic profiling for cancer diagnosis in dogs [PDF]
, 2016 Global gene expression, whereby tumours are classified according to similar gene expression patterns or ‘signatures’ regardless of cell morphology or tissue characteristics, is being increasingly used in both the human and veterinary fields to assist in ...
Morris, Joanna S.
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pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes
EuPA Open Proteomics, 2015 Gel-to-gel variation is a major problem in two-dimensional (2D) gel electrophoresis. Vertical and horizontal comparative fluorescence gel electrophoresis (v/hCoFGE) were recently introduced to improve the reproducibility of protein spot assignment. There,
Marina Hanneken +2 more
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Semi-denaturing Detergent Agarose Gel Electrophoresis (SDD-AGE)
Bio-Protocol, 2014 Pathological proteins in neurodegenerative diseases suffer a conformational change to a misfolded amyloid state. Such pathological event leads to the aggregation of these proteins that indefinitely propagates as an altered form of itself, and harbor ...
Laura Molina-García +1 more
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Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myelo\"id cell lines nuclear proteomes [PDF]
, 2012 One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear proteins lies in their strong interaction with nuclear acids.
Chevallet, Mireille +5 more
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Journal of Pharmacopuncture, 2006 Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide ...
Young Chon Choi +2 more
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Comparative Proteomics and Difference Gel Electrophoresis
BioTechniques, 2007 The goal of comparative proteomics is to analyze proteome changes in response to development, disease, or environment. This is a two-step process in which proteins within cellular extracts are first fractionated to reduce sample complexity, and then the ...
Jonathan Minden
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