Results 41 to 50 of about 1,156,214 (332)

Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myelo\"id cell lines nuclear proteomes [PDF]

, 2012
One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear proteins lies in their strong interaction with nuclear acids.
Chevallet, Mireille, Diemer, Hélène, Lelong, Cécile, Luche, Sylvie, Rabilloud, Thierry, Van Dorsselaer, Alain   +5 more
core   +4 more sources

C9orf72 ALS‐causing mutations lead to mislocalization and aggregation of nucleoporin Nup107 into stress granules

FEBS Letters, EarlyView.
Mutations in the C9orf72 gene represent the most common genetic cause of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease. Using patient‐derived neurons and C. elegans models, we find that the nucleoporin Nup107 is dysregulated in C9orf72‐associated ALS. Conversely, reducing Nup107 levels mitigates disease‐related changes.
Saygın Bilican, Yara Nabawi, William Hongyu Zhang, Dunja Petrovic, Markus Wehrmann, Sara Muñoz‐García, Seda Koyuncu, David Vilchez   +7 more
wiley   +1 more source

A new bond fluctuation method for a polymer undergoing gel electrophoresis

, 1998
We present a new computational methodology for the investigation of gel electrophoresis of polyelectrolytes. We have developed the method initially to incorporate sliding motion of tight parts of a polymer pulled by an electric field into the bond ...
B. H. Zimm, B. Norden, D. C. Schwartz, D. S. Mckenzie, G. W. Slater, H. Hervert, H. Oana, Hajime Takayama, I. Carmesin, J. Batoulis, J. M. Deutsch, J. M. Deutsch, N. C. Stellwagen, O. J. Lumpkin, Ryuzo Azuma, S. B. Smith, S. Gurrieri, T. D. Howard, W. D. Volkmuth   +18 more
core   +1 more source

Stochastic modeling for the COMET-assay [PDF]

, 2003
We present a stochastic model for single cell gel electrophoresis (COMET-assay) data. Essential is the use of point process structures, renewal theory and reduction to intensity histograms for further data ...
Boulesteix, Anne-Laure, Hösel, V., Liebscher, V.   +2 more
core   +3 more sources

Imeglimin attenuates liver fibrosis by inhibiting vesicular ATP release from hepatic stellate cells

FEBS Letters, EarlyView.
Imeglimin, at clinically relevant concentrations, inhibits vesicular ATP accumulation and release from hepatic stellate cells, thereby attenuating purinergic signaling and reducing fibrogenic activation. This mechanism reveals a newly identified antifibrotic action of imeglimin beyond glycemic control.
Seiji Nomura, Lixiang Wang, Nao Hasuzawa, Ayako Nagayama, Sawako Moriyama, Kenji Ashida, Yoshinori Moriyama, Masatoshi Nomura, Ken Yamamoto   +8 more
wiley   +1 more source

Protein electrophoretic migration data from custom and commercial gradient gels

Data in Brief, 2016
This paper presents data related to the article “A method for easily customizable gradient gel electrophoresis” (A.J. Miller, B. Roman, E.M. Norstrom, 2016) [1].
Andrew J. Miller, Brandon Roman, Eric M. Norstrom   +2 more
doaj   +1 more source

Cancer cell death induced by the NAD antimetabolite Vacor discloses the antitumor potential of SARM1

FEBS Letters, EarlyView.
Vacor, a compound converted into the toxic metabolite Vacor adenine dinucleotide (VAD) by the nicotinamide salvage pathway enzymes NAMPT and NMNAT2, exhibits antitumor activity by inducing rapid and complete NAD depletion. We report that Vacor toxicity is limited to cell lines expressing high levels of SARM1, a NAD glycohydrolase.
Giuseppe Ranieri, Andrea Lapucci, Giuseppe Orsomando, Nadia Raffaelli, Alberto Chiarugi, Daniela Buonvicino   +5 more
wiley   +1 more source

Reuse of Denaturing Polyacrylamide Gels for Short Tandem Repeat Analysis

BioTechniques, 1998
Denaturing polyacrylamide gel electrophoretic analysis of amplified polymorphic short tandem repeat (STR) loci using fluorescent markers is a mainstay of forensic and paternity testing.
Allan Tereba, Katherine A. Micka, James W. Schumm   +2 more
doaj   +1 more source

Proteomics-on-a-chip for Biomarker discovery [PDF]

, 2007
In proteomics research still two-dimensional gel electrophoresis (2D-GE) is currently used for biomarker discovery. We applied free flow electrophoresis (FFE) separation technology combined with biomolecular interaction sensing using Surface Plasmon ...
Berg, Albert van den, Eijkel, Jan, Kohlheyer, Dietrich, Schasfoort, Richard B.M., Schlautmann, Stefan   +4 more
core   +1 more source

Revealing the structure of land plant photosystem II: the journey from negative‐stain EM to cryo‐EM

FEBS Letters, EarlyView.
Advances in cryo‐EM have revealed the detailed structure of Photosystem II, a key protein complex driving photosynthesis. This review traces the journey from early low‐resolution images to high‐resolution models, highlighting how these discoveries deepen our understanding of light harvesting and energy conversion in plants.
Roman Kouřil
wiley   +1 more source