Results 51 to 60 of about 1,126,750 (353)

Protein electrophoretic migration data from custom and commercial gradient gels

Data in Brief, 2016
This paper presents data related to the article “A method for easily customizable gradient gel electrophoresis” (A.J. Miller, B. Roman, E.M. Norstrom, 2016) [1].
Andrew J. Miller, Brandon Roman, Eric M. Norstrom   +2 more
doaj   +1 more source

Guidelines for reporting the use of gel electrophoresis in proteomics [PDF]

Nat Biotechnol 26, 8 (2008) 863-4, 2009
the MIAPE Gel Electrophoresis (MIAPE-GE) guidelines specify the minimum information that should be provided when reporting the use of n-dimensional gel electrophoresis in a proteomics experiment. Developed through a joint effort between the gel-based analysis working group of the Human Proteome Organisation's Proteomics Standards Initiative (HUPO-PSI ...
arxiv   +1 more source

In vivo evidence for glycyl radical insertion into a catalytically inactive variant of pyruvate formate‐lyase

FEBS Letters, EarlyView.
Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel, A. F. Volker Wagner, R. Gary Sawers   +2 more
wiley   +1 more source

Application of Pulsed Field Gel Electrophoresis to Determine γ-ray-induced Double-strand Breaks in Yeast Chromosomal Molecules [PDF]

, 1993
The frequency of DNA double-strand breaks (dsb) was determined in yeast cells exposed to γ-rays under anoxic conditions. Genomic DNA of treated cells was separated by pulsed field gel electrophoresis, and two different approaches for the evaluation of ...
A.A. Friedl, A.M. Kellerer, Ager D.D., Ager D.D., Ahn S.Y., Andrews J., Blazek E.R., Blöcher D, Blöcher D, Blöcher D., Blöcher D., Blöcher D., Bronstein I., Contopoulou C.R., F. Eckardt-Schupp, Frankenberg D., Frankenberg-Schwager M., Geigl E.-M., Geigl E.-M., Geigl E.-M., Geigl E.-M., Hanawalt P.C, Ho K.S.Y, Iliakis G.E., Iliakis G.E., Jacobs A., K. Hahn, Kessler B., Kunz B.A., Lennartz M., Okayasu R.B., Oleinick N.L., Radford I.R., Resnick M.A., Schulz G.V, Smith L.C., Stamato T.D., Terleth C., W. Beisker, Ward J.F   +39 more
core   +1 more source

Reuse of Denaturing Polyacrylamide Gels for Short Tandem Repeat Analysis

BioTechniques, 1998
Denaturing polyacrylamide gel electrophoretic analysis of amplified polymorphic short tandem repeat (STR) loci using fluorescent markers is a mainstay of forensic and paternity testing.
Allan Tereba, Katherine A. Micka, James W. Schumm   +2 more
doaj   +1 more source

Development of Pipetting Devices to Separate Protein Complexes [PDF]

arXiv, 2022
The objective of this project is to develop an automated device used to spot protein samples on a hydrophobic membrane to be used for the patented electrophoresis method developed by Chang and Yonan in 2008 [1]. This novel method performs electrophoresis directly on hydrophobic blot membranes as opposed to the previous popular methods such as the 2-D ...
arxiv  

Unlocking the potential of tumor‐derived DNA in urine for cancer detection: methodological challenges and opportunities

Molecular Oncology, EarlyView.
Urine is a rich source of biomarkers for cancer detection. Tumor‐derived material is released into the bloodstream and transported to the urine. Urine can easily be collected from individuals, allowing non‐invasive cancer detection. This review discusses the rationale behind urine‐based cancer detection and its potential for cancer diagnostics ...
Birgit M. M. Wever, Renske D. M. Steenbergen   +1 more
wiley   +1 more source

Capabilities and limitations of gel electrophoresis for elemental speciation : a laboratory's experience [PDF]

, 2006
Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper is based on four years of practical experience of the authors' lab in this domain
Chéry, Cyrille C, Cornelis, Rita, Moens, Luc, Vanhaecke, Frank   +3 more
core   +2 more sources

Transcriptome‐wide analysis of circRNA and RBP profiles and their molecular relevance for GBM

Molecular Oncology, EarlyView.
CircRNAs are differentially expressed in glioblastoma primary tumors and might serve as therapeutic targets and diagnostic markers. The investigation of circRNA and RNA‐binding proteins (RBPs) interactions shows that distinct RBPs play a role in circRNA biogenesis and function.
Julia Latowska‐Łysiak, Żaneta Zarębska, Marcin P. Sajek, Adriana Grabowska, Alessia Buratin, Paweł Głodowicz, Julia O. Misiorek, Konrad Kuczyński, Stefania Bortoluzzi, Marek Żywicki, Jan G. Kosiński, Agnieszka Rybak‐Wolf, Rafał Piestrzeniewicz, Anna M. Barciszewska, Katarzyna Rolle   +14 more
wiley   +1 more source

Agarose Gel Analysis of 15–40-kb PCR Amplimers

BioTechniques, 1996
Accurate resolution of PCR products in the range of 15–40 kb may be obtained in agarose gels without pulsed-field electrophoresis. A gel of 0.3% SeaKem® Gold agarose cast on GelBond® support film provides good resolution and sufficient gel strength to ...
Valerie Burland, Foner P. Curtis, Noriko Kusukawa   +2 more
doaj   +1 more source