Results 51 to 60 of about 1,156,214 (332)

MADGE: Microplate array diagonal gel electrophoresis [PDF]

Journal of Medical Biochemistry, 2009
Microplate array diagonal gel electrophoresis (MADGE) was invented for molecular genetic epidemiological studies. MADGE is a highly flexible, cost effective, microplate compatible solution to high throughput electrophoresis needs.
Stanković Sanja, Alavantić Dragan, Majkić-Singh Nada   +2 more
doaj  

Rad27/FEN1 prevents accumulation of Okazaki fragments and ribosomal DNA copy number changes

FEBS Letters, EarlyView.
The budding yeast Rad27 is a structure‐specific endonuclease. Here, the authors reveal that Rad27 is crucial for maintaining the stability of the ribosomal RNA gene (rDNA) region. Rad27 deficiency leads to the accumulation of Okazaki fragments and changes in rDNA copy number.
Tsugumi Yamaji, Yuko Katayama, Nanase Arata, Mariko Sasaki   +3 more
wiley   +1 more source

Agarose Gel Analysis of 15–40-kb PCR Amplimers

BioTechniques, 1996
Accurate resolution of PCR products in the range of 15–40 kb may be obtained in agarose gels without pulsed-field electrophoresis. A gel of 0.3% SeaKem® Gold agarose cast on GelBond® support film provides good resolution and sufficient gel strength to ...
Valerie Burland, Foner P. Curtis, Noriko Kusukawa   +2 more
doaj   +1 more source

Study of protein expresion [sic] in peri-infarct tissue after cerebral ischemia [PDF]

, 2015
In this work, we report our study of protein expression in rat peri-infarct tissue, 48 h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal ...
Agulla, Jesús, Blanco, Miguel, Brea, David, Campos, Francisco, Castillo, José, Dávalos, Antoni, Gevaert, Kris, Ramos-Cabrer, Pedro, Sobrino, Tomás, Staes, An   +9 more
core   +5 more sources

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

An Improved Method for PAGE-based Detection of Phosphorylated Protein in Yeast

Bio-Protocol, 2012
One dimensional polyacrylamide gel electrophoresis has been successfully used to detect protein phosphorylation. This method is very simple and highly reproducible.
Yuehua Wei
doaj   +1 more source

Gel-Electrophoresis and Diffusion of Ring-Shaped DNA

, 1997
A model for the motion of ring-shaped DNA in a gel is introduced and studied by numerical simulations and a mean-field approximation. The ring motion is mediated by finger-shaped loops (hernias) that move in an amoeba-like fashion around the gel ...
A. R. Kholkov, B. Alberts, B. H. Zimm, B. H. Zimm, C. Godréche, C. Heller, D. C. Schwartz, David Mukamel, E. M. Sevick, G. F. Carle, G. Parisi, G. W. Slater, J. Klein, J. M. Deutsch, J. P. de Gennes, M. Doi, M. E. Cates, M. R. Evans, M. R. Evans, M. Rubinstein, M. Wang, O. J. Lumpkin, P. Serwer, S. B. Smith, S. Gurrieri, S. Levene, S. Mickel, S. P. Obukhov, T. A. J. Duke, T. A. J. Duke, T. A. J. Duke, T. Duke, U. Alon, Uri Alon, W. D. Volkmuth, W. D. Volkmuth   +35 more
core   +2 more sources

Synthetic metallomolecules as agents for the control of DNA structure [PDF]

, 2007
This tutorial review summarises B-DNA structure and metallomolecule binding modes and illustrates some DNA structures induced by molecules containing metallic cations. The effects of aquated metal ions, cobalt amines, ruthenium octahedral metal complexes,
Adair D. Richards, Alessio, Alison Rodger, Baschnagel, Bierbach, Boulikas, Bragg, Brey, Browning, Carle, Chargaff, Clarke, Coggan, den Hartog, Eichhorn, Gale, Gessner, Goodsell, Guschlbauer, Hannon, Hannon, Haq, Hiort, Holliday, Humphrey, Khalid, Laine, Lerman, Lerman, Levitt, Liu, Loprete, Mainwaring, Manzotti, Marrington, Meistermann, Miescher, Molderheim, Neidle, Oleksi, Parkinson, Parkinson, Peberdy, Pelta, Rodger, Rodger, Rodger, Rodger, Rodger, Rosenberg, Sherman, Tabor, Ushay, Watson, Zou   +54 more
core   +1 more source

Tonic signaling of the B‐cell antigen‐specific receptor is a common functional hallmark in chronic lymphocytic leukemia cell phosphoproteomes at early disease stages

Molecular Oncology, EarlyView.
B‐cell chronic lymphocytic leukemia (B‐CLL) and monoclonal B‐cell lymphocytosis (MBL) show altered proteomes and phosphoproteomes, analyzed using mass spectrometry, protein microarrays, and western blotting. Identifying 2970 proteins and 316 phosphoproteins, including 55 novel phosphopeptides, we reveal BCR and NF‐kβ/STAT3 signaling in disease ...
Paula Díez, Pablo Juanes‐Velasco, Marina L. García‐Vaquero, Conrad Droste, Alicia Landeira‐Viñuela, Miguel Alcoceba, Helena Fidalgo‐Gómez, Sara Misiego‐Herrero, Almudena Navarro‐Bailón, Mónica Baile, José M. Bastida, Jose Manuel Sanchez‐Santos, Rafael Góngora, Julia Almeida, Marcos Gonzalez‐Diaz, Alberto Orfao, Javier De Las Rivas, Manuel Fuentes   +17 more
wiley   +1 more source

Compensation Of Smile Effect Distortion In Electrophoretic Gel Image [PDF]

, 2015
This paper is engaged in the issue of automatic detection and removal of smile effect geometrical distortion in agarose gel electrophoresis images. Based on created databank of electrophoretic phantoms, an algorithm that is able to repair mentioned smile
Dvořáček, T.
core