Results 81 to 90 of about 412,145 (282)
BMC Bioinformatics, 2004 Background Many proteomics initiatives require a seamless bioinformatics integration of a range of analytical steps between sample collection and systems modeling immediately assessable to the participants involved in the process. Proteomics profiling by
Arthur John +4 more
doaj +1 more source
Magnetophoresis of Tagged Polymers
, 1996 We present quantitative results for the drift velocity of a polymer in a gel if a force (e.g. through an electric or magnetic field) acts on a tag, attached to one of its ends.
Barkema, G. T., Schütz, G. M.
core +2 more sources
A two-dimensional electrophoresis protocol suitable for Medicago truncatula leaf proteome [PDF]
, 2015 Medicago truncatula leaves were used as the experimental materials. Total proteins of leaves were extracted by trichloracetic acid (TCA)-acetone method and proteins had a better separation using gel strips, forming an immobilized non-linear 3 to 10 pH ...
Dong, Wen +7 more
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Mapping Hsp104 interactions using cross‐linking mass spectrometry
FEBS Open Bio, EarlyView.This study examines how cross‐linking mass spectrometry can be utilized to analyze ATP‐induced conformational changes in Hsp104 and its interactions with substrates. We developed an analytical pipeline to distinguish between intra‐ and inter‐subunit contacts within the hexameric homo‐oligomer and discovered contacts between Hsp104 and a selected ...
Kinga Westphal +3 more
wiley +1 more source
Mini Gel Electrophoresis Control Software and System Design With Python
Gazi Üniversitesi Fen Bilimleri Dergisi, 2019 Electrophoresis is the method of separating charged macromolecules such as DNA, RNA and Protein molecules by moving them from one pole to the other, between (-) and (+) charged poles, in an electric field.
Ahmet Reşit KAVSAOĞLU +1 more
doaj +1 more source
Diffusion constant for the repton model of gel electrophoresis
, 1997 The repton model is a simple model of the "reptation" motion by which DNA diffuses through a gel during electrophoresis. In this paper we show that the model can be mapped onto a system consisting of two types of particles with hard-sphere interactions ...
Barkema, G. T., Newman, M. E. J.
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Localization of the genes for tumor necrosis factor and lymphotoxin between the HLA classI and III regions by field inversion gel electrophoresis [PDF]
, 1988 To clarify the position of the TNFA and TNFB genes on the HLA map, we have assigned TNFA to large DNA restriction fragments separated by field inversion gel electrophoresis, which hybridize with either class III- or class I-specific probes as well. These
Bloemer, Katharina +3 more
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FEBS Open Bio, EarlyView.The C. elegans tetraspanin‐7 (tsp‐7) is a homologue of human CD63, which is a negative regulator of autophagy. The C. elegans strain, tm5761, has a dysfunctional (knockout) tsp‐7 gene. When compared to the wild‐type strain, the tm5761 strain shows increased: life‐ and health‐span; thermotolerance, and stress‐induced locomotion.
Brogan Jones +2 more
wiley +1 more source
BioTechniques, 2014 Protein acetylation and phosphorylation are key modifications that regulate both normal and pathological protein functions. The gel systems currently used for analyzing modified proteins require either expensive reagents or time-consuming second ...
Christopher J. Buehl +6 more
doaj +1 more source
Establishment and biological characterization of radioresistant colorectal cancer cell lines
FEBS Open Bio, EarlyView.Under ionizing radiation exposure, radiation‐sensitive cancer cells exhibit oxidative stress and DNA damage, while radiation‐resistant cancer cells exhibit strong antioxidant properties and DNA damage repair. Radiotherapy resistance is a major cause of recurrence and metastasis in colorectal cancer (CRC).
Tian‐Yin Qu +10 more
wiley +1 more source