Results 81 to 90 of about 1,156,214 (332)
Sequence-specific double-strand cleavage of DNA by penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [PDF]
, 1983 In the presence of O2 and 5 mM dithiothreitol, penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [P5E·Fe(II)] at 0.5 µ M cleaves pBR322 plasmid DNA (50 µ M in base pairs) on opposite strands to afford discrete DNA fragments as analyzed by agarose gel ...
Dervan, Peter B., Schultz, Peter G.
core
Mitochondrial proteomics: analysis of a whole mitochondrial extract with two-dimensional electrophoresis [PDF]
, 2008 Mitochondria are complex organelles, and their proteomics analysis requires a combination of techniques. The emphasis in this chapter is made first on mitochondria preparation from cultured mammalian cells, then on the separation of the mitochondrial ...
A. Gorg +16 more
core +5 more sources
Bridging the gap: Multi‐stakeholder perspectives of molecular diagnostics in oncology
Molecular Oncology, EarlyView.Although molecular diagnostics is transforming cancer care, implementing novel technologies remains challenging. This study identifies unmet needs and technology requirements through a two‐step stakeholder involvement. Liquid biopsies for monitoring applications and predictive biomarker testing emerge as key unmet needs. Technology requirements vary by
Jorine Arnouts +8 more
wiley +1 more source
pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes
Data in Brief, 2015 Amphoteric azo dyes were used for internal control of pI values in Comparative two-dimensional Fluorescence Gel Electrophoresis (CoFGE) [1]. The 2D-gel images of separated Escherichia coli proteins as well as those of colored amphoteric dyes separated by
Marina Hanneken +2 more
doaj +1 more source
Fluorescent Visualization of In Vitro Mitochondrial DNA Transcription [PDF]
, 2019 Mitochondria are important organelles within eukaryotic cells especially for their role in metabolism and ATP production by the oxidative phosphorylation (OXPHOS) pathway. In human cells there are approximately 80 protein subunits that make up the OXPHOS
Bruce, Kelsey
core +1 more source
Transport in polymer-gel composites: Theoretical methodology and response to an electric field
, 2006 A theoretical model of electromigrative, diffusive and convectivetransport polymer-gel composites is presented. Bulk properties are derived from the standard electrokinetic model with an impenetrable charged sphere embedded in an electrolyte-saturated ...
Hill, Reghan J.
core +1 more source
Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia [PDF]
, 1971 Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-3H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide ...
Wilson, David L.
core +4 more sources
Molecular Oncology, EarlyView.In this exploratory study, we investigated the relationship between the gut microbiota and outcome in patients with metastatic hormone receptor‐positive breast cancer, treated in a randomized clinical trial with chemotherapy alone or chemotherapy in combination with immune checkpoint blockade.
Andreas Ullern +7 more
wiley +1 more source
Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients
Brazilian Journal of Infectious Diseases, 2013 Burkholderia cenocepacia may cause serious infections in patients with cystic fibrosis, and this microorganism can be highly transmissible. Pulsed-field gel electrophoresis is widely used to study the dynamics of strain spread in cystic fibrosis patients.
Luana Pretto +4 more
doaj +3 more sources
BMC Bioinformatics, 2004 Background Many proteomics initiatives require a seamless bioinformatics integration of a range of analytical steps between sample collection and systems modeling immediately assessable to the participants involved in the process. Proteomics profiling by
Arthur John +4 more
doaj +1 more source