Results 151 to 160 of about 916,712 (391)
Advanced Biology, EarlyView.A detailed workflow for recombinant GALC production and characterization is presented to support enzyme replacement therapy for Krabbe disease. In vitro assays demonstrate that physiological GALC doses restore enzymatic activity and autophagic flux without affecting cell viability, whereas higher doses impair autophagy and reduce viability.
Ambra Del Grosso +5 more
wiley +1 more source
Heterogeneity Between Two Mouse-Toxic Protein Polymers from Pasteurella pestis Indicated by Electrophoresis Patterns in a Phenol-Acetic Acid-Urea Gel System [PDF]
, 1969 Thomas C. Montie, Diane B. Montie
openalex +1 more source
Bioorthogonal Engineering of Cellular Microenvironments Using Isonitrile Ligations
Advanced Functional Materials, EarlyView.Highly selective chemistries are required for fabrication and post‐cross–linking modification of cell‐encapsulating hydrogels used in tissue engineering applications. Isonitrile ligation reactions represent a promising class of bioorthogonal chemistries for engineering hydrogel‐based cellular microenvironments. Isonitrile‐based hydrogels are stable and
Ping Zhou +2 more
wiley +1 more source
Electroblotting onto activated glass. High efficiency preparation of proteins from analytical sodium dodecyl sulfate-polyacrylamide gels for direct sequence analysis [PDF]
, 1986 We have developed a new method for the isolation of proteins for microsequencing. It consists of electrophoretic transfer (electroblotting) of proteins or their cleavage fragments onto activated glass filter paper sheets immediately after separation by ...
Aebersold, Ruedi H. +3 more
core
Advanced Functional Materials, EarlyView.The MPTT‐nanozyme‐hydrogel system (Mn3O4@ChS‐HA) provides a multifunctional therapeutic strategy for intervertebral disc degeneration (IVDD), effectively targeting oxidative stress and enhancing AF repair by restoring extracellular matrix (ECM) and redox homeostasis.
Yangyang Chen +13 more
wiley +1 more source
Cell Membrane Vesicle Camouflaged Artificial Cells
Advanced Functional Materials, Volume 35, Issue 30, July 24, 2025.Artificial cells camouflaged with a cell membrane vesicle coating are able to assemble into synthetic aggregates that exhibit rudimentary communication capabilities. Additionally, when these artificial cells are equipped with antioxidant capabilities, they are able to protect the intracellular homeostasis in HepG2 cells present in semi‐synthetic ...
Paula De Dios Andres +11 more
wiley +1 more source
Characterization of Proteins from Nucleolar Preribosomes of Mouse Leukemia Cells by Two‐Dimensional Polyacrylamide Gel Electrophoresis [PDF]
, 1978 Marie-Annick Auger-Buendia, M. Longuet
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Advanced Functional Materials, EarlyView.Oct4‐nanoscript, a biomimetic nanoparticle‐based artificial transcription factor, precisely regulates cellular rejuvenation by activating Oct4 target genes, restoring epigenetic marks, and reducing DNA damage. In a progeria model, it effectively rescued aging‐associated pathologies and extended lifespan.
Hongwon Kim +8 more
wiley +1 more source
Examination of the Protein Composition of the Cell Envelope ofEscherichia coliby Polyacrylamide Gel Electrophoresis [PDF]
, 1970 Carl A. Schnaitman
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Advanced Functional Materials, EarlyView.The temporary transition of macrophages from a pro‐inflammatory phenotype of macrophages (M1) to an anti‐inflammatory phenotype of macrophages (M2) is crucial for tissue repair and regeneration processes. Bacterial outer membrane vesicles (OMVs) are utilized as a “trojan horse” for specific M1 macrophage‐targeting and anti‐inflammatory drug delivery ...
Donglin Cai +9 more
wiley +1 more source