Electrophoresis of Unlabeled Proteins in a Sequencing Gel Apparatus
Claudio Luparello
doaj +1 more source
Characterization of Proteins from Nucleolar Preribosomes of Mouse Leukemia Cells by Two‐Dimensional Polyacrylamide Gel Electrophoresis [PDF]
Marie-Annick Auger-Buendia, M. Longuet
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Hydrophobin‐Coated Echogenic Microbubbles for Molecular Targeting of Tumor Cells
Echogenic microbubbles based on hydrophobin and corresponding fusion protein (HFBI‐domZ) are developed as contrast agents for cancer diagnostics. Hydrophobin provides high stabilization of encapsulated perfluoro‐n‐butane, and fusion proteins allow further versatile functionalization with antibodies. In‐vitro experiments indicate that these microbubbles
Hedar H. Al‐Terke+8 more
wiley +1 more source
CHARACTERIZATION OF BOAR SEMINAL PLASMA, VESICULAR SECRETION AND EPIDIDYMAL PLASMA PROTEINS BY GEL DISC ELECTROPHORESIS AND ISOELECTRIC FOCUSING ON POLYACRYLAMIDE [PDF]
U. Lavon, J. C. Boursnell
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FUS Selectively Facilitates circRNAs Packing into Small Extracellular Vesicles within Hypoxia Neuron
This study aimed to unravel the role of FUS in the loading of functional circRNAs into neuronal sEVs under hypoxic conditions, which involves their aggregations in SGs and specific interaction between the FUS Zf_RanBP domain to regulate circRNA sorting in sEVs.
Jiankun Zang+14 more
wiley +1 more source
Sodium dodecyl sulfate electrophoresis of high molecular weight proteins in N, N'‐diallyltartardiamide‐cross‐linked polyacrylamide slab gels [PDF]
Andreas Ziegler, Hans Hengartner
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AAV2.7m8 serotype combined with the gfaABC1D promoter targets infection of supporting cells (SCs). AAV2.7m8‐gfaABC1D‐Gjb2 administration to mice results in excessive immune responses. The combination of AAV2.7m8‐gfaABC1D‐Gjb2 with dexamethasone (DEX) shows a synergistic effect and enhances the gene therapy effect in a conditional Cx26 null mice model ...
Xiaohui Wang+8 more
wiley +1 more source
Bilirubin Targeting WNK1 to Alleviate NLRP3‐Mediated Neuroinflammation
At physiological concentrations, bilirubin binds to the kinase domain of WNK1, thereby augmenting its activity and facilitating the phosphorylation of downstream SPAK/OSR1. This phosphorylation inhibits KCC2 activity, leading to elevate intracellular chloride levels in neurons.
Linfei Mao+14 more
wiley +1 more source
Quantitation of eukaryotic ribosomal proteins separated by two‐dimensional polyacrylamide gel electrophoresis [PDF]
Oskar H. W. MARTINI+4 more
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This study reveals that histone lactylation, a modification derived from lactate, plays a crucial role in ferroptosis resistance in ectopic endometrial stromal cells. This resistance is mediated via the HIF1A/HMOX1 pathway regulated by METTL3. In vivo, a combination of 2‐DG and erastin shows promise as an effective treatment for endometriosis ...
Zongwen Liang+8 more
wiley +1 more source