Results 231 to 240 of about 3,576,469 (305)

Gel Electrophoresis of Proteins and Enzymes

1976
Proteins are defined as large molecular weight substances (104 – 105) composed of amino acids coupled by the α carboxyl group of one acid and the α amino group of another forming a polypeptide:
F. Bergmann, P. P. Feret
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Rapid capillary gel electrophoresis of proteins

Journal of Chromatography A, 1993
The rapid separation of sodium dodecyl sulphate-protein complexes according to their molecular masses (M(r)) by capillary gel electrophoresis is described. Using commercial equipment, standard proteins with M(r) in the range 29,000-97,400 were resolved to the baseline in less than 2 min by utilizing a separation distance of 7 cm.
J. Schlösser, O. W. Reif, Ruth Freitag, Thomas Scheper, J. Fleischer, Ralf Dr. Lausch   +5 more
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Starch Gel Electrophoresis of Proteins

Methods in molecular biology, 2003
Starch gel is one of a wide variety of supporting media that can be used for horizontal zone electrophoresis. Such gels are prepared by heating and cooling a quantity of partially hydrolyzed starch in an appropriate buffer solution. The choice of buffer is somewhat empirical and a wide variety of compositions have been used successfully.
Graham B. Divall
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SDS Polyacrylamide Gel Electrophoresis of Proteins

1996
Crosslinked polyacrylamide gels are formed from the polymerization of acrylamide monomer in the presence of smaller amounts of N,N'-methylene-bis-acrylamide (normally referred to as “bis-acrylamide”) (Fig. 1). Note that bis-acrylamide is essentially two acrylamide molecules linked by a methylene group and is used as a crosslinking agent.
J. Walker
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One‐Dimensional Gel Electrophoresis of Proteins

Current Protocols in Immunology, 1991
AbstractThis unit provides several methods for one‐dimensional gel electrophoresis of proteins. The basic protocol is the standard method used for gel electrophoresis under denaturing conditions. The first alternate protocol provides conditions used for nondenaturing gel electrophoresis.
Sean R. Gallagher, John A. S. Smith
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Two-dimensional gel electrophoresis of membrane proteins

Biochemistry, 1976
A high-resolution method for two-dimensional separation of membrane proteins is described. It involves a nondiscriminating solubilization of a membrane preparation with sodium dodecyl sulfate, followed by electrophoresis in the first dimension according to charge (by isoelectric focusing).
Kishiko Nikaido, Giovanna Ferro-Luzzi Ames   +1 more
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Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins

Methods in molecular biology, 1996
J. Walker
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Gel electrophoresis of proteins: A practical approach

Immunology Today, 1982
R. Calvert
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One‐Dimensional SDS Gel Electrophoresis of Proteins

Current Protocols in Molecular Biology, 2012
One‐dimensional gel electrophoresis of proteins provides information about the molecular size, amount, and purity of a protein sample. Separated proteins can be recovered from polyacrylamide gels for subsequent characterization by a variety of secondary ...
S. Gallagher
semanticscholar   +2 more sources

One‐Dimensional SDS Gel Electrophoresis of Proteins

Current Protocols in Molecular Biology, 1995
Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or immunoprecipitates), to investigate subunit compositions, and to verify homogeneity of protein samples.
S. Gallagher
semanticscholar   +1 more source