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Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins

2003
Since O'Farrell (1) introduced the improved technique for high resolution two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), it has become one of the most powerful tools for the separation and quantification of proteins from complex mixtures. The principal reason for this is that the method employs separation of denatured proteins according
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Polyacrylamide Gel Electrophoresis of Plant Proteins

1974
Development is characterized by an ordered, controlled sequence of changes that occur within an organism at the molecular, cell, tissue and organ levels. Differentiation of cells and tissues involves processes that give rise to a variety of cell types within different tissues. Such cells within a particular tissue or organ are characterized by a unique
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Artifacts and Common Errors in Protein Gel Electrophoresis

2018
In spite of taking precautions, some common mistakes creep into well-planned gel electrophoresis experiments. This occurs commonly in relation to calculating the cross-linking factor of a gel, polymerization temperature and time for a polyacrylamide gel, inducing aggregates in samples for electrophoresis, titrating the running buffer in electrophoresis,
Biji T. Kurien   +5 more
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Gel electrophoresis of protein–DNA complexes

Nature, 1987
Regions of DNA bending can be explored using gel electrophoresis on protein-DNA complexes, proving gel electrophoresis does more than just separate proteins and nucleic acids according to size.
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Protein Detection in Polyacrylamide Gel Electrophoresis

1987
An investigator must take several factors into account for the process of staining and recording the location of bands after electrophoresis (Wilson, 1979, 1983). These should include (1) the selection and availability of an appropriate dye of satisfactory quality that will give adequate sensitivity; (2) the selection of a solvent that will provide a ...
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