Results 21 to 30 of about 855,961 (311)

Isolation and Analytical Characterization of Local Malaysian Leech Saliva

International Islamic University Malaysia Engineering Journal, 2011
Leech saliva contains biologically active compounds that are mainly proteins and peptides. In this study a modified and smooth extraction method of saliva was used without leeches' scarification.
Mohamed Alaama, Manar AlNajjar, Abdualrahman Abdualkader, Abbas Mohammad, and Ahmed Merzouk   +4 more
doaj   +1 more source

Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis

Proteomes, 2016
The pioneering work by Patrick H. O’Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry 1975, 250, 4007–4021).
Sandra Murphy, Paul Dowling, Kay Ohlendieck   +2 more
doaj   +1 more source

Carbohydrate Affinity PAGE for the Study of Carbohydrate-Binding Proteins

BioTechniques, 1998
Immobilized neoglycoconjugates covalently cross-linked into a polyacrylamide gel can be used to detect and characterize carbohydrate-binding proteins.
Phil D. Rye, Nicolai V. Bovin
doaj   +1 more source

Gel Electrophoresis of Proteins [PDF]

, 2012
Gel electrophoresis is a widely known group of techniques used to separate and identify macromolecules as DNA, RNA, or proteins based on size, form, or isoelectric point. The separation of molecules by electrophoresis is based on the fact that charged molecules migrate through a gel matrix upon application of an electric field.
Laura Garca-Descalzo, Eva Garca-Lpez, Alberto Alczar, Fernando Baquero, Cristina Ci   +4 more
openaire   +1 more source

Nigrosine staining of wheat endosperm proteolipid patterns on starch gels [PDF]

, 1975
We have previously characterized a group of proteolipids from wheat endosperm, designated CM proteins, which are soluble in chloroformmethanol (2:1, v/v) and have a molecular weight lower than 25,000 daltons (1-3).
Aragoncillo, Boyd, C. Aragoncillo, Coulson, Elton, Ewart, F. Garcia-Olmedo, Feillet, Garcia Olmedo, Garcia Olmedo, Graham, Jennings, M.A. Rodriguez-Loperena, Mita, Pilar Carbonero, Redman, Solari, Woychick, Wrigley, Yong   +19 more
core   +2 more sources

Mapping cells and sub-cellular organelles on 2-D gels: ‘new tricks for an old horse’ [PDF]

, 1995
Nowadays, investigators in all fields are faced with the identification of unknown, up- or down-regulated, modified proteins that they are trying to identify.
Dahlbäck, Björn,, de Vries, R., Dullaart, R. P. F., Groen, A. K., Lund University., Nielsen, L. B., Plomgaard, P.   +6 more
core   +4 more sources

Analysis of 953 human proteins from a mitochondrial HEK293 fraction by complexome profiling. [PDF]

PLoS ONE, 2013
Complexome profiling is a novel technique which uses shotgun proteomics to establish protein migration profiles from fractionated blue native electrophoresis gels.
Hans J C T Wessels, Rutger O Vogel, Robert N Lightowlers, Johannes N Spelbrink, Richard J Rodenburg, Lambert P van den Heuvel, Alain J van Gool, Jolein Gloerich, Jan A M Smeitink, Leo G Nijtmans   +9 more
doaj   +1 more source

A Comparison of gel (Hydragel 30) and capillary (Capillarys III Tera) electrophoresis for the characterization of human serum proteins

Practical Laboratory Medicine, 2021
Objectives: To compare gel (Hydrasys 2 from Sebia) and capillary (Capillarys III Tera, Sebia) electrophoresis for the characterization of human serum proteins.
Brittney M. Howard, Annie Kuh, LuAnn Rezavi, Patrizio Caturegli   +3 more
doaj   +1 more source

Application of Fluorescence Two-Dimensional Difference In-Gel Electrophoresis as a Proteomic Biomarker Discovery Tool in Muscular Dystrophy Research

Biology, 2013
In this article, we illustrate the application of difference in-gel electrophoresis for the proteomic analysis of dystrophic skeletal muscle. The mdx diaphragm was used as a tissue model of dystrophinopathy.
Steven Carberry, Margit Zweyer, Dieter Swandulla, Kay Ohlendieck   +3 more
doaj   +1 more source

A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting

Antibodies, 2022
In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the ...
Teruo Akuta, Toshiaki Maruyama, Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Kevin Entzminger, Jonathan K. Fleming, Ryo Sato, Takashi Shibata, Yasunori Kurosawa, C. J. Okumura, Tsutomu Arakawa   +11 more
doaj   +1 more source