Results 21 to 30 of about 168,112,797 (204)

The guideline for western blotting assay

Food Frontiers, 2022
Western blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody
Fang Wang, Gaohong Yang, Haizhao Song, Ling Xiong, Luanfeng Wang, Xinchun Shen   +5 more
doaj   +1 more source

Image Analysis Workflow for 2-D Electrophoresis Gels Based on ImageJ [PDF]

, 2011
A number of commercial software packages are currently available to perform digital two-dimensional electrophoresis (2D-GE) gel analysis. However, both the high cost of the commercial packages and the unavailability of a standard data analysis workflow ...
Bucci, Em, Enrico M. Bucci, Paolo Abrescia, Natale, Massimo, Massimo Natale, Bernardetta Maresca, Abrescia, P., Maresca, B.   +7 more
core   +1 more source

Application of Pulsed Field Gel Electrophoresis to Determine γ-ray-induced Double-strand Breaks in Yeast Chromosomal Molecules [PDF]

, 1993
The frequency of DNA double-strand breaks (dsb) was determined in yeast cells exposed to γ-rays under anoxic conditions. Genomic DNA of treated cells was separated by pulsed field gel electrophoresis, and two different approaches for the evaluation of ...
Beisker, W., Eckardt-Schupp, F., Kellerer, Albrecht M., Eckard-Schupp, F., Friedl, A.A., Kellerer, A.M., Hahn, K.R., Friedl, Anna A., Hahn, K., K. Hahn, A.M. Kellerer, F. Eckardt-Schupp, A.A. Friedl, W. Beisker   +13 more
core   +1 more source

Resolving acetylated and phosphorylated proteins by neutral urea Triton-polyacrylamide gel electrophoresis: NUT-PAGE

BioTechniques, 2014
Protein acetylation and phosphorylation are key modifications that regulate both normal and pathological protein functions. The gel systems currently used for analyzing modified proteins require either expensive reagents or time-consuming second ...
Christopher J. Buehl, Xiexiong Deng, Mengyu Liu, Michael J McAndrew, Stacy Hovde, Xinjing Xu, Min-Hao Kuo   +6 more
doaj   +1 more source

Protein electrophoretic migration data from custom and commercial gradient gels

Data in Brief, 2016
This paper presents data related to the article “A method for easily customizable gradient gel electrophoresis” (A.J. Miller, B. Roman, E.M. Norstrom, 2016) [1].
Andrew J. Miller, Brandon Roman, Eric M. Norstrom   +2 more
doaj   +1 more source

Discontinuous native protein gel electrophoresis [PDF]

ELECTROPHORESIS, 2006
AbstractAnalysis of the oligomeric state of a native protein usually requires analytical ultracentrifugation or repeated gel filtration to calculate the protein's size. We have developed a discontinuous native protein gel electrophoresis system that allows the separation of even basic proteins according to their size, oligomeric state, and shape.
Michael, Niepmann, Junfeng, Zheng
openaire   +2 more sources

Protein concentration of synovial fluid in chronic rheumatoid arthritis. Estimation of protein in the synovial fluid of chronic rheumatoid arthritis by gel filtration and paper electrophoresis [PDF]

, 1968
For the purpose to reveal the characteris6cs of the synovial fluid of the chronic rheumatoid arthritis the proteins of the synovial fluid and blood serum have been analysed by employing the methods of electrophoresis, gel filtration on Sephadex G-200 ...
Seno, Takashi
core   +1 more source

Isolation and Analytical Characterization of Local Malaysian Leech Saliva

International Islamic University Malaysia Engineering Journal, 2011
Leech saliva contains biologically active compounds that are mainly proteins and peptides. In this study a modified and smooth extraction method of saliva was used without leeches' scarification.
Mohamed Alaama, Manar AlNajjar, Abdualrahman Abdualkader, Abbas Mohammad, and Ahmed Merzouk   +4 more
doaj   +1 more source

Western blotting of native proteins from agarose gels

BioTechniques, 2022
We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Toshiaki Maruyama, Kevin Entzminger, Jonathan K Fleming, Takashi Shibata, Yasunori Kurosawa, CJ Okumura, Tsutomu Arakawa, Teruo Akuta   +10 more
doaj   +1 more source

Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat [PDF]

, 2010
Low-molecular-weight glutenin subunits (LMW-GS) play a crucial role in determining end-use quality of common wheat by influencing the viscoelastic properties of dough.
Branlard, Gerard, Rogers, William John, Liu, Li, Ikeda, Tatsuya M, Wang Aili, Yoshida, H., Branlard, G., Xia, X., Kolman, Maía de Los Angeles, Roberto J Peña, Ma, Wujun, Ma, W., Rogers, William J, Wang, A., Lerner, S.E., Gerard Branlard, Zhonghu He, Lerner, Silvia E., He, Zhonghu, Yan, Y., Wang Linhai, Linhai Wang, Lerner Silvia E, Wang, Aili, He, Z., Silvia E Lerner, Rogers William J, Wujun Ma, Branlard, Gerard,, Peña, R., Kolman María A, Xia Xianchun, William J Rogers, Appels, R., Kolman, María A, Yoshida Hisashi, Kolman, M.A., Peña, Roberto J, Yoshida, Hisashi, Rogers, W.J., Liu, L., Lerner, Silvia E, Tatsuya M Ikeda, Ma Wujun, Branlard, Gerard, G., Wang, L., Yan, Yueming, Wang, Linhai, Appels, Rudi, Kolman, Maria de Los Angeles, Hisashi Yoshida, Appels Rudi, He Zhonghu, Peña Roberto J, Liu Li, Aili Wang, Ikeda Tatsuya M, María A Kolman, Peña, R.J., Li Liu, Yan Yueming, Xia, Xianchun, Ikeda, T.M., Yueming Yan, Rogers, John William, Xianchun Xia, Branlard Gerard, Rudi Appels, Ikeda, Tatsuya M., Peña, Roberto J.   +69 more
core   +1 more source