Results 21 to 30 of about 576,652 (264)

Western Blot Analysis of Chloroplast HSP70B in Chlorella Species

Bio-Protocol, 2013
Western blotting allows for the specific detection of proteins by an antibody of interest. This protocol utilizes isolation of total proteins protocol for Chlorella vulgaris prior to gel electrophoresis. After electrophoresis, the selected antibodies are
Stephka Chankova, Zhana Mitrovska, Nadezhda Yurina   +2 more
doaj   +1 more source

Analysis of 953 human proteins from a mitochondrial HEK293 fraction by complexome profiling. [PDF]

PLoS ONE, 2013
Complexome profiling is a novel technique which uses shotgun proteomics to establish protein migration profiles from fractionated blue native electrophoresis gels.
Hans J C T Wessels, Rutger O Vogel, Robert N Lightowlers, Johannes N Spelbrink, Richard J Rodenburg, Lambert P van den Heuvel, Alain J van Gool, Jolein Gloerich, Jan A M Smeitink, Leo G Nijtmans   +9 more
doaj   +1 more source

Polysaccharide as a Separation Medium for Gel Electrophoresis

Polysaccharides
Gel electrophoresis and size exclusion chromatography (SEC) are vital techniques in biochemical research, employing gel matrix structures made of polysaccharides or synthetic polymers like polyacrylamide for the analysis and separation of macromolecules.
Tsutomu Arakawa, Masataka Nakagawa, Chiaki Sakuma, Yui Tomioka, Yasunori Kurosawa, Teruo Akuta   +5 more
doaj   +1 more source

Total Protein Extraction from Cultured Cells for Use in Electrophoresis and Western Blotting

BioTechniques, 1996
Experimentation with cultured cells often requires analyzing cellular protein extract by gel electrophoresis and immunoblotting. Traditional methods for extracting cellular proteins by homogenization or detergent solubilization usually produce protein ...
Kevin K.W. Wang, Avigail Posner, Iradj Hajimoharmmadreza   +2 more
doaj   +1 more source

A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting

Antibodies, 2022
In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the ...
Teruo Akuta, Toshiaki Maruyama, Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Kevin Entzminger, Jonathan K. Fleming, Ryo Sato, Takashi Shibata, Yasunori Kurosawa, C. J. Okumura, Tsutomu Arakawa   +11 more
doaj   +1 more source

False-Negative Urine Protein Electrophoresis by Semiautomated Gel Electrophoresis [PDF]

Clinical Chemistry, 2004
Urine is evaluated by electrophoretic methods both to gather information about the location and degree of damage within the nephron and to detect and quantify monoclonal free light chains (Bence Jones proteins). For many years, urine protein electrophoresis consisted of concentrating the urine (usually >50-fold) followed by manual application of the ...
David F, Keren, Ronald, Gulbranson, Stephen J, Ebrom   +2 more
openaire   +2 more sources

Efficacy of gel electrophoresis for proteins and biotechnological products –an overview.

Al-Mustansiriyah Journal of Pharmaceutical Sciences, 2022
Nucleic acids or proteins electrophoresed within a matrix or gel that immersed in a buffer provides ions needed to carry a current and for pH maintenance at a relatively constant value. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis   (
Ammar Q. Muhammed, Nidhal K. Maraie, Basma T. Al-Sudani   +2 more
doaj   +1 more source

Protein heterogeneity of lipoprotein particles containing apolipoprotein A-I without apolipoprotein A-II and apolipoprotein A-I with apolipoprotein A-II isolated from human plasma.

Journal of Lipid Research, 1988
The protein heterogeneity of fractions isolated by immunoaffinity chromatography on anti-apolipoprotein A-I and anti-apolipoprotein A-II affinity columns was analyzed by high resolution two-dimensional gel electrophoresis.
R W James, D Hochstrasser, J D Tissot, M Funk, R Appel, F Barja, C Pellegrini, A F Muller, D Pometta   +8 more
doaj   +1 more source

Identification of antigenic proteins from salivary glands of female Anopheles maculatus by proteomic analysis

Asian Pacific Journal of Tropical Biomedicine, 2016
Objective: To identify antigenic proteins from the salivary glands of female Anopheles maculatus using a proteomic approach to find the biomarker candidate for serological tools.
Yunita Armiyanti, Renam Putra Arifianto, Elisa Nurma Riana, Kartika Senjarini, Widodo Widodo, Loeki Enggar Fitri, Teguh Wahju Sardjono   +6 more
doaj   +1 more source

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source