Results 41 to 50 of about 855,961 (311)

Mitochondrial proteomics: analysis of a whole mitochondrial extract with two-dimensional electrophoresis [PDF]

, 2008
Mitochondria are complex organelles, and their proteomics analysis requires a combination of techniques. The emphasis in this chapter is made first on mitochondria preparation from cultured mammalian cells, then on the separation of the mitochondrial ...
A. Gorg, C. Tastet, D. Mokranjac, F. Gharahdaghi, F. Granier, H. Zischka, J. C. Sanchez, M. Chevallet, M. Eschenbruch, P. Lescuyer, S. Iwata, S. Luche, S. Luche, S. Richert, T. Rabilloud, T. Tsukihara, V. Santoni   +16 more
core   +6 more sources

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Alterations of the mitochondrial proteome caused by the absence of mitochondrial DNA: A proteomic view [PDF]

, 2006
The proper functioning of mitochondria requires that both the mitochondrial and the nuclear genome are functional. To investigate the importance of the mitochondrial genome, which encodes only 13 subunits of the respiratory complexes, the mitochondrial ...
Abdul, Appleby, Armstrong, Bai, Bourges, Buchet, Chai, Claros, Da Cruz, Dey, Du, Garcia, Gianazza, Joseph, King, King, Lescuyer, Luche, Manjasetty, Martinus, Perkins, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Santoni, Sharma, Sharp, Sherer, Singh, Sinha, Suzuki, Tastet, Taylor, Tryoen-Toth, Ugalde, Wiedemann, Zamzami, Zischka   +39 more
core   +4 more sources

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Elution of proteins from polyacrylamide eels: a simple and economic procedure

Revista do Instituto de Medicina Tropical de São Paulo, 1989
A simplified methodology for the quantitative electroelution of proteins from polyacrylamide gels is described. After staining with Coomassie Brilliant Blue R 250, the identified bands are excised from the gel and the proteins eluted using a procedure ...
Vera Bongertz
doaj   +1 more source

Gene induction during differentiation of human monocytes into dendritic cells: an integrated study at the RNA and protein levels [PDF]

, 2001
Changes in gene expression occurring during differentiation of human monocytes into dendritic cells were studied at the RNA and protein levels. These studies showed the induction of several gene classes corresponding to various biological functions ...
Angénieux, C., Bausinger, H., Cazenave, J. P., de la Salle, H., Fricker, D., Hanau, D., Luche, S., Rabilloud, T., Strub, J. M., Van Dorsselaer, A.   +9 more
core   +3 more sources

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Western Analysis of Histone Modifications (Aspergillus nidulans)

Bio-Protocol, 2013
Western blotting allows for the specific detection of proteins and/or modifications of proteins by an antibody of interest. This protocol utilizes a crude nuclei extraction protocol for Aspergillus nidulans to enrich for histones and other nuclear ...
Alexandra Soukup, Nancy Keller
doaj   +1 more source

Detergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis [PDF]

, 2006
Because of the outstanding separating capabilities of two-dimensional electrophoresis for complete proteins, it would be advantageous to be able to apply it to all types of proteins.
Chevallet, Mireille, Luche, Sylvie, Rabilloud, Thierry, Santoni, Véronique   +3 more
core   +4 more sources

Transcriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication

Molecular Oncology, EarlyView.
Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice, Jose I. Lopez, Aitziber Ugalde‐Olano, Maite Zufiaurre, Ianire Astobiza, Natalia Martin‐Martin, Laura Bozal‐Basterra, Saioa Garcia‐Longarte, Amaia Zabala‐Letona, Sofia Rey, Aida Santos‐Martin, Miguel Unda, Ana Loizaga‐Iriarte, Mariona Graupera, Paolo Nuciforo, Arkaitz Carracedo, Isabel Mendizabal   +16 more
wiley   +1 more source