Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis
Proteomes, 2016 The pioneering work by Patrick H. O’Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry 1975, 250, 4007–4021).
Sandra Murphy +2 more
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Carbohydrate Affinity PAGE for the Study of Carbohydrate-Binding Proteins
BioTechniques, 1998 Immobilized neoglycoconjugates covalently cross-linked into a polyacrylamide gel can be used to detect and characterize carbohydrate-binding proteins.
Phil D. Rye, Nicolai V. Bovin
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Gel Electrophoresis of Proteins [PDF]
, 2012 Gel electrophoresis is a widely known group of techniques used to separate and identify macromolecules as DNA, RNA, or proteins based on size, form, or isoelectric point. The separation of molecules by electrophoresis is based on the fact that charged molecules migrate through a gel matrix upon application of an electric field.
Laura Garca-Descalzo +4 more
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Enhanced levels of cold shock proteins in Listeria monocytogenes LO28 upon exposure to low temperature and high hydrostatic pressure [PDF]
, 2002 Listeria monocytogenes is a psychrotrophic food-borne pathogen that is problematic for the food industry because of its ubiquitous distribution in nature and its ability to grow at low temperatures and in the presence of high salt concentrations.
Karatzas, Andreas K. +7 more
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Analysis of the effects of BrdU on DLKP human lung cancer cells by two-dimensional difference gel electrophoresis and mass spectrometry [PDF]
, 2007 Bromodeoxyuridine (BrdU) is a thymidiie analogue that incorporates into DNA of dividing cells during the S-phase of the cell cycle. Previous work in laboratories reported that treatment with lOyM BrdU in the human lung carcinoma cell line (DLKP) resulted
Henry, Michael
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Protein purification by Off-Gel electrophoresis [PDF]
PROTEOMICS, 2002 A novel free-flow protein purification technique based on isoelectric electrophoresis is presented, where the proteins are purified in solution without the need of carrier ampholytes. The gist of the method is to flow protein solutions under an immobilised pH gradient gel (IPG) through which an electric field is applied perpendicular to the direction ...
Ros, A. +8 more
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Localization of the genes for tumor necrosis factor and lymphotoxin between the HLA classI and III regions by field inversion gel electrophoresis [PDF]
, 1988 To clarify the position of the TNFA and TNFB genes on the HLA map, we have assigned TNFA to large DNA restriction fragments separated by field inversion gel electrophoresis, which hybridize with either class III- or class I-specific probes as well. These
Bloemer, K +7 more
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Biology, 2013 In this article, we illustrate the application of difference in-gel electrophoresis for the proteomic analysis of dystrophic skeletal muscle. The mdx diaphragm was used as a tissue model of dystrophinopathy.
Steven Carberry +3 more
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Analysis of 953 human proteins from a mitochondrial HEK293 fraction by complexome profiling. [PDF]
PLoS ONE, 2013 Complexome profiling is a novel technique which uses shotgun proteomics to establish protein migration profiles from fractionated blue native electrophoresis gels.
Hans J C T Wessels +9 more
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Practical Laboratory Medicine, 2021 Objectives: To compare gel (Hydrasys 2 from Sebia) and capillary (Capillarys III Tera, Sebia) electrophoresis for the characterization of human serum proteins.
Brittney M. Howard +3 more
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