Application of Denaturing Gradient Gel ElectrophoresisApplication of Denaturing Gradient Gel Electrophoresis(DGGE) Methods on Parent-Offspring Relationship of the Coral Pocillopora damicornis [PDF]
DGGE (Denaturing Gradient Gel Electrophoresis) is the most powerful methods for mutation detection currentlyavailable. In DGGE, DNA fragments of the same length but with different sequences can be separated.
Permata W, Diah, Hirose, M, Hidaka , M
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Objective: To identify antigenic proteins from the salivary glands of female Anopheles maculatus using a proteomic approach to find the biomarker candidate for serological tools.
Yunita Armiyanti +6 more
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Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) [PDF]
QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.
Bernd Kastenholz
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Elution of proteins from polyacrylamide eels: a simple and economic procedure
A simplified methodology for the quantitative electroelution of proteins from polyacrylamide gels is described. After staining with Coomassie Brilliant Blue R 250, the identified bands are excised from the gel and the proteins eluted using a procedure ...
Vera Bongertz
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Genomic relatedness within five common Finnish Campylobacter jejuni pulsed-field gel electrophoresis genotypes studied by amplified fragment length polymorphism analysis, ribotyping and serotyping [PDF]
Thirty-five Finnish Campylobacter jejuni strains with five SmaI/SacII pulsed-field gel electrophoresis (PFGE) genotypes selected among human and chicken isolates from 1997 and 1998 were used for comparison of their PFGE patterns, amplified fragment ...
Hanninen, M.L. +4 more
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Western Analysis of Histone Modifications (Aspergillus nidulans)
Western blotting allows for the specific detection of proteins and/or modifications of proteins by an antibody of interest. This protocol utilizes a crude nuclei extraction protocol for Aspergillus nidulans to enrich for histones and other nuclear ...
Alexandra Soukup, Nancy Keller
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Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide ...
Young Chon Choi +2 more
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pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes
Amphoteric azo dyes were used for internal control of pI values in Comparative two-dimensional Fluorescence Gel Electrophoresis (CoFGE) [1]. The 2D-gel images of separated Escherichia coli proteins as well as those of colored amphoteric dyes separated by
Marina Hanneken +2 more
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Proteomic analysis of the heat shock and acclimation responses of Cyanobacteria [PDF]
The cyanobacterium Synechocystis sp. PCC 6803 is a model experimental organism for proteomic research because its entire genomic sequence is available and cyanobacteria have high adaptive potential towards a variety of environmental stresses.
Hall, John James
core
Here we describe the isolation and purity determination of Trypanosoma brucei (T. b.) brucei candidate target proteins of artemisinin. The candidate target proteins were detected and purified from their biological source (T. b.
Benetode Konziase
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