Results 211 to 220 of about 864,053 (256)
Some of the next articles are maybe not open access.
Is Gene Amplification RNA-directed?
Nature New Biology, 1973Gene amplification in oocytes has been thought to involve a “reverse transcriptase” like that found in the RNA tumour viruses. Neither the template RNA nor the hybrid intermediates required by this theory can be detected in amplifying ovaries.
A, Bird, E, Rogers, M, Birnstiel
openaire +2 more sources
Regulation and mechanisms of gene amplification
Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, 1995Abstract Amplification in rodent cells usually involves bridge-breakage-fusion (bbf) cycles initiated either by endto-end fusion of sister chromatids, or by chromosome breakage. In contrast, in human cells, resistance to the antimetabolite A-(phosphonacetyl)-L-aspartate (PALA) can be mediated by several different mechanisms that lead ...
K A, Smith +8 more
openaire +2 more sources
Induction of Gene Amplification by Arsenic
Science, 1988Arsenic is a well-established carcinogen in humans, but there is little evidence for its carcinogenicity in animals and it is inactive as an initiator or tumor promoter in two-stage models of carcinogenicity in mice. Two arsenic salts (sodium arsenite and sodium arsenate) induced a high frequency of methotrexate-resistant 3T6 cells, which were shown to
T C, Lee +4 more
openaire +2 more sources
Alternative Model for Gene Amplification
Nature New Biology, 1971The number of nucleoli increases shortly after the conversion of germ cells into oogonia or spermatogonia in Xenopus laevis. This event demands a modification of the theory of gene amplification. A new explanation is proposed which involves non-chromosomal genes restricted to the germ-line.
H, Wallace, J, Morray, W H, Langridge
openaire +2 more sources
Gene Amplification and qRT-PCR
2014This chapter includes methods for the use of the polymerase chain reaction (PCR) with Pseudomonas, and several specific tips for their successful application in this organism. The first part of the chapter includes methods for purifying genomic DNA from, and amplifying genes from, Pseudomonas, in addition to methods which describe how to prepare a cell
Cerith, Jones, Alain, Filloux
openaire +2 more sources
Gene amplification and tumor progression
Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1993Proto-oncogenes are the genes which are most frequently found amplified in human tumor cells. Acquisition of a drug-resistant phenotype by gene amplification is frequent for in-vitro cultured cells but is very rare in human tumors. Proto-oncogenes amplified in human tumors belong essentially to one of three families (erbB, ras, myc) or to the 11q13 ...
openaire +2 more sources
Gene amplification and gene correction in somatic cells
Cell, 1982We used gene transfer to identify frequent genetic rearrangements responsible for activating mutant genes in mammalian cells. We transformed an aprt- tk- cell with a plasmid containing a wild-type aprt gene and a truncated, promoterless tk gene. Transformants that integrate a single copy of this plasmid exhibit the aprt+ phenotype but remain tk-.
J M, Roberts, R, Axel
openaire +2 more sources
Gene amplification in human gliomas
Glia, 1995AbstractGliomas represent the largest group of primary brain tumors in adults. The astrocytic variants are the most common and the adult forms are histologically stratified into three malignancy grades. Of these glioblastoma is the most common and the most malignant; it has also been best studied by molecular genetics and cytogenetics.
openaire +2 more sources