Results 181 to 190 of about 54,378 (282)
What Makes an “Ideal” Cell Line for Recombinant Adeno‐Associated Virus Production?
Biotechnology and Bioengineering, EarlyView.Several host cell types have been used to produce rAAVs to date. Cell line‐specific traits that are beneficial in the context of rAAV manufacturing are reviewed here, with the goal of developing a consensus on the ‘ideal’ characteristics that an rAAV production host should possess (created with Biorender.com).
James Conheady +6 more
wiley +1 more source
CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors. [PDF]
Cell Rep, 2017 Bak RO, Porteus MH.
europepmc +1 more source
Fouling‐Free Inertial Microfluidic Cell Clarification for Lentivirus Manufacturing
Biotechnology and Bioengineering, EarlyView.ABSTRACT Lentiviral vectors (LVVs) are widely used as gene‐delivery vehicles in cell and gene therapy, enabling stable integration of transgenes into target cells. However, efficient clarification of LVV harvest remains a major bottleneck in large‐scale manufacturing, as conventional depth filtration often results in significant product loss.
Dohyun Park +5 more
wiley +1 more source
BMEMat, EarlyView.A dual strategy integrating genetic engineering and physical encapsulation was proposed to enhance the cell viability and antioxidant properties of probiotics. The cascade antioxidant enzymes released from probiotic microspheres restore intestinal redox homeostasis by eliminating intestinal reactive oxygen species for ulcerative colitis treatment ...
Xiaolong Mao +9 more
wiley +1 more source
The power of many: when genetics met yeasts and high‐throughput
Biological Reviews, EarlyView.ABSTRACT In recent years, complex technological capabilities have evolved, driven by the need to solve complex and integrative biological questions through global analyses. New equipment allows the scaling up and automation of processes which previously were carried out on a very limited scale.
Víctor A. Tallada, Víctor Carranco
wiley +1 more source
Versatile nourseothricin and streptomycin/spectinomycin resistance gene cassettes and their use in chromosome integration vectors. [PDF]
J Microbiol Methods, 2016 Lehman SS +5 more
europepmc +1 more source
A Novel Gene Cassette Potentially Conferring Resistance to Aminoglycosides [PDF]
Antimicrobial Agents and Chemotherapy, 2012 Sally R, Partridge, Guy, Tsafnat
openaire +2 more sources
RNA‐centric world of retroviruses: unravelling the molecular strategies of genomic RNA packaging
Biological Reviews, EarlyView.ABSTRACT Retroviruses constitute a unique group of RNA viruses that have profoundly influenced both evolutionary trajectories and biomedical research. Their ability to reverse transcribe and integrate into host genomes has shaped genomic architecture across species and contributed to our understanding of oncogenes, gene regulation, and RNA biology ...
Mohammad Abdullah Jehad +5 more
wiley +1 more source
Episomal Expression Cassettes For Gene Therapy
, 2007 The Invention Consists Of Episomal Expression Cassettes For Expression Of A Transgene In Gene Therapy. The Expression Cassettes Consist Of Regulatory Elements Of The Human Cytokeratin Gene And A Transgene. The Invention Also Includes Of Liposomes For Transfection Of Epithelial Tissue With The Cassettes In Treatment Of Cystic Fibrosis, Emphysema ...
openaire +1 more source
Status and future of recombinant adeno‐associated virus vector manufacturing
Biotechnology Progress, EarlyView.Abstract Sixty years of adeno‐associated virus (AAV) research illustrates a trajectory marked by basic science exploration, iterative innovation, persistent challenges, a number of clinical setbacks, as well as commercial therapeutic triumphs. This continual evolution has led to recombinant AAV (rAAV) becoming a cornerstone of modern gene therapy ...
Frank Agbogbo, David Dismuke
wiley +1 more source