Results 61 to 70 of about 3,039,728 (253)

Construction and application of an insertional activation cloning vector

BMC Biotechnology
Background Foreign DNA cloning is a critical step in genetic engineering and molecular biology for acquiring target genes. Efficient and accurate identification of positive clones from transformants is essential to this process.
Yan Zhang, Yun He, Yi Ding, Shanhua Lyu, Yinglun Fan   +4 more
doaj   +1 more source

DNA ligase LigE increases bacterial transformation with damaged extracellular DNA

FEBS Letters, EarlyView.
The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan, Avi Singh, Shrawani Weralupe Gamladdalage, Joanna Hicks, Adele Williamson   +4 more
wiley   +1 more source

SnTox3 acts in effector triggered susceptibility to induce disease on wheat carrying the Snn3 gene [PDF]

, 2009
The necrotrophic fungus Stagonospora nodorum produces multiple proteinaceous host-selective toxins (HSTs) which act in effector triggered susceptibility.
McDonald, B., McDonald Megan C., Nunez, A., Faris Justin D., Liu, Z., Zhaohui Liu, Friesen Timothy L., Tan, Kar-Chun, Oliver, R.P., Faris, Justin D., Kar-Chun Tan, Oliver, Richard P., Rasmussen, Jack B., Lu Shunwen, Solomon Peter S., Richard P Oliver, Friesen, T., Tan, K.C., Megan C McDonald, McDonald, M., Oliver Richard P., Bruce A McDonald, Friesen, T.L., Jack B Rasmussen, McDonald, Megan C., McDonald, Megan C.; id_orcid, Faris, J.D., Peter S Solomon, Liu Zhaohui, Nunez, Alberto, McDonald, Bruce A., Rasmussen, J., Rasmussen Jack B., Friesen, Timothy L., Faris, J., McDonald Bruce A., Oliver, Richard, Liu, Zhaohui, McDonald, B.A., Rasmussen, J.B., Nunez Alberto, Solomon, Peter S., Tan Kar-Chun, Timothy L Friesen, Solomon, P., McDonald, M.C., Lu, S., Shunwen Lu, Justin D Faris, Alberto Nunez, Lu, Shunwen, Solomon, P.S.   +51 more
core   +1 more source

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

Human Cloning Research. National Academy of Sciences Forum (2001 August 7) (Part 1)

, 2001
The scientists provided background information on a variety of topics, including embryology, scientific issues underlying cloning, and the role of mitochondria.
Producer: National Cable Satellite Corporation. C-SPAN
core  

Circular RNA expression landscapes in myelodysplastic neoplasms: Associations with mutational signatures and disease progression

Molecular Oncology, EarlyView.
In this explorative study, the abundance of circular RNA molecules in bone marrow stem cells was found to be elevated in patients with high‐risk myelodysplastic neoplasms, and to be associated with an increased risk of progression to acute myeloid leukemia.
Eileen Wedge, Morten Tulstrup, Gabriele Todisco, Pedro Moura, Christophe Côme, Jakob Werner Hansen, Jakob Schmidt Jespersen, Balthasar Schlotmann, Maria Creignou, Mette Dahl, Claudia Schöllkopf, Klas Raaschou‐Jensen, Krister Wennerberg, Bo Porse, Joachim Weischenfeldt, Eva Hellström‐Lindberg, Lasse Sommer Kristensen, Kirsten Grønbæk   +17 more
wiley   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Hijacking emergency granulopoiesis: Neutrophil ontogeny and reprogramming in cancer

Molecular Oncology, EarlyView.
Neutrophils are highly plastic innate immune cells; their functions in cancer extend beyond the tumour microenvironment. This Review summarises current understanding of neutrophil maturation and heterogeneity and highlights tumour‐induced granulopoiesis as a systemic programme that expands immature, immunosuppressive neutrophils via tumour‐derived ...
Gabriela Marinescu, Yi Feng
wiley   +1 more source

Mutagenesis and Gene Cloning in Schizosaccharomyces pombe Using Nonhomologous Plasmid Integration and Rescue

BioTechniques, 2000
Genes are commonly cloned in yeasts and bacteria by plasmid complementation, where the introduction of the gene of interest into a host strain carrying a recessive mutation in that gene suppresses the host’s mutant phenotype.
Charles S. Hoffman, Robert Welton
doaj   +1 more source

Stimulator of interferon genes agonist augmented antitumor immunity of osimertinib in Egfr‐mutated lung cancer

Molecular Oncology, EarlyView.
Combining osimertinib with the STING agonist ADU‐S100 activates innate and adaptive immunity to overcome the non‐inflamed microenvironment of Egfr‐mutant lung cancer. This combination increases NK and CD8+ T‐cell infiltration, associated with activation of the STING‐IRF3 pathway and local immunogenic cell death.
Jun Nishimura, Tadahiro Kuribayashi, Johannes Brägelmann, Sachi Okawa, Masataka Taoka, Shunta Mori, Tomoka Nishimura, Takaaki Tanaka, Go Makimoto, Kiichiro Ninomiya, Kammei Rai, Eiki Ichihara, Ryohei Katayama, Katsuyuki Hotta, Masahiro Tabata, Yosuke Togashi, Yoshinobu Maeda, Martin L. Sos, Katsuyuki Kiura, Kadoaki Ohashi   +19 more
wiley   +1 more source